IGEM:MIT/2007/Protocols: Difference between revisions
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*[[../Notebook/2007-6-12#Protocol for Preparing to Run a Gel Electrophoresis|Preparing a Gel Electrophoresis]] | *[[../Notebook/2007-6-12#Protocol for Preparing to Run a Gel Electrophoresis|Preparing a Gel Electrophoresis]] | ||
*[[../Notebook/2007-6-12#Protocol for Running a Gel Electrophoresis|Running a Gel Electrophoresis]] | *[[../Notebook/2007-6-12#Protocol for Running a Gel Electrophoresis|Running a Gel Electrophoresis]] | ||
*[[../Notebook/2007-6-13# | *[[../Notebook/2007-6-13#Making_LB_Amp_Agar_Plates|Making LB Amp Agar Plates]] | ||
*[[../Notebook/2007-6-11#innoculate_5ml_LB/Amp_with_bact._colonies_(EGFP_and_NNX)|Innoculating LB with bacteria]] | *[[../Notebook/2007-6-11#innoculate_5ml_LB/Amp_with_bact._colonies_(EGFP_and_NNX)|Innoculating LB with bacteria]] | ||
*[[../Notebook/2007-6-12#Protocol for Imaging Result and Gel Diagnostic|Gel Imaging]] | *[[../Notebook/2007-6-12#Protocol for Imaging Result and Gel Diagnostic|Gel Imaging]] | ||
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*[[../Notebook/2007-6-13#Purifying_the_DNA|Purifying the DNA]] | *[[../Notebook/2007-6-13#Purifying_the_DNA|Purifying the DNA]] | ||
*[[../Notebook/2007-6-14#Transformation_Protocol|Transformation]] | *[[../Notebook/2007-6-14#Transformation_Protocol|Transformation]] | ||
*[[../Notebook/2007-7-19#Colony PCR Protocol (OWW, Knight)|Colony PCR (OWW, Knight)]] | |||
*[[Phosphatase_treatment_of_linearized_vector|Phosphatase treatment of linearized vector]] | |||
Latest revision as of 08:10, 20 July 2007
General Lab Protocols
- [[../Notebook/2007-6-11#General_Lab_Do_s_and_Don’t_s|General Lab Do's and Don'ts]]
- [[../Notebook/2007-6-12#Nanodrop (~20ng/ul) Protocol|Calculating Optical Density; Nanodrop Protocol]]
- [[../Notebook/2007-6-12#Obtaining a Cast|Making a Cast]]
- [[../Notebook/2007-6-11#made_1_liter_1%_agarose_solution|Making Agarose Solution]]
- [[../Notebook/2007-6-12#Protocol for Preparing Agarose Gel|Making an Agarose Gel]]
- [[../Notebook/2007-6-12#Protocol for Preparing to Run a Gel Electrophoresis|Preparing a Gel Electrophoresis]]
- [[../Notebook/2007-6-12#Protocol for Running a Gel Electrophoresis|Running a Gel Electrophoresis]]
- [[../Notebook/2007-6-13#Making_LB_Amp_Agar_Plates|Making LB Amp Agar Plates]]
- [[../Notebook/2007-6-11#innoculate_5ml_LB/Amp_with_bact._colonies_(EGFP_and_NNX)|Innoculating LB with bacteria]]
- [[../Notebook/2007-6-12#Protocol for Imaging Result and Gel Diagnostic|Gel Imaging]]
DNA Work
- [[../Notebook/2007-6-12#Mini Prep|Mini Prep]]
- [[../Notebook/2007-6-18#Mini-prep|Mini Prep v.2.0]]
- [[../Notebook/2007-6-12#PCR_amplification_of_EGFP|PCR Amplification]]
- [[../Notebook/2007-6-12#PCR_purify(microcentrifuge)|PCR Purification in a Microcentrifuge]]
- [[../Notebook/2007-6-12#PCR Cleanup|PCR Cleanup]]
- [[../Notebook/2007-6-12#Notes for Double Digestion|Double Digestion]]
- [[../Notebook/2007-6-13#Ligation|Ligation]]
- [[../Notebook/2007-6-13#Purifying_the_DNA|Purifying the DNA]]
- [[../Notebook/2007-6-14#Transformation_Protocol|Transformation]]
- [[../Notebook/2007-7-19#Colony PCR Protocol (OWW, Knight)|Colony PCR (OWW, Knight)]]
- Phosphatase treatment of linearized vector
