IGEM:MIT/2007/Protocols: Difference between revisions
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== Protocols == | == Protocols == | ||
=== PCR === | |||
Platinum PCR Supermix | |||
# Set Reaction Tubes/Plates on Ice | |||
# Add the following components in a reaction vessel | |||
* 45 µl Platinum PCR SuperMix | |||
* Primers (200 nM final concentration per primer is recommended) | |||
* Template DNA solution | |||
Total volume should be between .5-20 µl. | |||
# Cap reaction vessel and load into a | |||
Revision as of 10:25, 12 June 2007
Protocols
PCR
Platinum PCR Supermix
- Set Reaction Tubes/Plates on Ice
- Add the following components in a reaction vessel
- 45 µl Platinum PCR SuperMix
- Primers (200 nM final concentration per primer is recommended)
- Template DNA solution
Total volume should be between .5-20 µl.
- Cap reaction vessel and load into a
