IGEM:MIT/2007/Protocols
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Protocols
PCR
Platinum PCR Supermix
- Set Reaction Tubes/Plates on Ice
- Add the following components in a reaction vessel
- 45 µl Platinum PCR SuperMix
- Primers (200 nM final concentration per primer is recommended)
- Template DNA solution
Total volume should be between .5-20 µl.
- Cap reaction vessel and load into a thermal cycler at 94°C
- Incubate tubes in a thermal cycler at 94°C for 30 s to 2 min
- Perform 25-35 cycles of PCR amplification
- Denature 94° for 15-30 s
- Anneal 55°C for 15-30 s
- Extend 72°C for 1 min per kb