IGEM:MIT/2007/Protocols

General Lab Protocols

• [[../Notebook/2007-6-11#General_Lab_Do_s_and_Don’t_s|General Lab Do's and Don'ts]]
• [[../Notebook/2007-6-12#Nanodrop (~20ng/ul) Protocol|Calculating Optical Density; Nanodrop Protocol]]
• [[../Notebook/2007-6-12#Obtaining a Cast|Making a Cast]]
• [[../Notebook/2007-6-11#made_1_liter_1%_agarose_solution|Making Agarose Solution]]
• [[../Notebook/2007-6-12#Protocol for Preparing Agarose Gel|Making an Agarose Gel]]
• [[../Notebook/2007-6-13#Plating_on_Agar|Plating on Agar]]
• [[../Notebook/2007-6-11#innoculate_5ml_LB/Amp_with_bact._colonies_(EGFP_and_NNX)|Innoculating LB with bacteria]]

DNA Work

• [[../Notebook/2007-6-12#Mini Prep|Mini Prep]]
• [[../Notebook/2007-6-12#PCR_amplification_of_EGFP|PCR Amplification]]
• [[../Notebook/2007-6-12#PCR_purify(microcentrifuge)|PCR Purification in a Microcentrifuge]]
• [[../Notebook/2007-6-13#Ligation|Ligation]]
• [[../Notebook/2007-6-13#Purifying_the_DNA|Purifying the DNA]]
• [[../Notebook/2007-6-14#Transformation_Protocol|Transformation]]