IGEM:MIT/2007/Signalers

Display Proteins

• mRFP Derivatives
  • mRFP1 The original
  • tdTomato [1]
    • Brightest
    • Best quantum Yield
    • Double the molecular weight though
  • mCherry [2]
    • Longest wavelengths
    • Highest photostability
    • Fastest maturation
    • Excellent pH resistance
    • Quantum efficiency is slightly lower (0.22 versus 0.25 for mRFP1)
    • Higher extinction coefficient (due to near-complete maturation)
    • Tolerance of N-terminal fusions and photostability make mRFP1 obsolete

1. Shaner NC, Campbell RE, Steinbach PA, Giepmans BN, Palmer AE, and Tsien RY. Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nat Biotechnol. 2004 Dec;22(12):1567-72. DOI:10.1038/nbt1037 |