IGEM:MIT/2007/Signalers

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Promoter Sequences

Mercury

Display Proteins

  • mRFP Derivatives
    • mRFP1 The original
    • tdTomato [1]
      • Brightest
      • Best quantum Yield
      • Double the molecular weight though
    • mCherry [2]
      • Longest wavelengths
      • Highest photostability
      • Fastest maturation
      • Excellent pH resistance
      • Quantum efficiency is slightly lower (0.22 versus 0.25 for mRFP1)
      • Higher extinction coefficient (due to near-complete maturation)
      • Tolerance of N-terminal fusions and photostability make mRFP1 obsolete
  1. Shaner NC, Campbell RE, Steinbach PA, Giepmans BN, Palmer AE, and Tsien RY. . pmid:15558047. PubMed HubMed [Shaner04]
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