IGEM:MIT/2007/Updated Ideas: Difference between revisions

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** A yeast sensor of ligand binding [http://www.nature.com/nbt/journal/v19/n11/abs/nbt1101-1042.html;jsessionid=DA353F52D612DC9BB63737E61CD7DC52]
** A yeast sensor of ligand binding [http://www.nature.com/nbt/journal/v19/n11/abs/nbt1101-1042.html;jsessionid=DA353F52D612DC9BB63737E61CD7DC52]
** Biosensor for estrogen in water samples [http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6TFC-4H21K6M-1&_user=501045&_coverDate=02%2F15%2F2006&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000022659&_version=1&_urlVersion=0&_userid=501045&md5=24d7c0b492c7a1c96e1ad878ce98c607]
** Biosensor for estrogen in water samples [http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6TFC-4H21K6M-1&_user=501045&_coverDate=02%2F15%2F2006&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000022659&_version=1&_urlVersion=0&_userid=501045&md5=24d7c0b492c7a1c96e1ad878ce98c607]
==sticky project:==
input:
*temperature (cold shock / heat shock promoters)
*light (either via light sensitive transcription/translation or photo-induced chemistry)
*cell growth (stationary phase, log phase)
*cell type (if growing different strains)
*metal ion (metal sensitive promoter)
*estrogen (ER binding domain)
parts:
*mussel peptide fused to circularly permuted OmpX (surface display protein, see Rice07)
**need tyrosine hydroxylase to convert tyrosine to L-DOPA (hydroxylation)?  can also occur non-enzymatically (reversible)
**need tyrosinase to convert L-DOPA to DOPA-quinone (oxidation)?  can also occur non-enzymatically (reversible) -- heavy metal ions (like copper) can catalyze...
*polystyrene peptide fused to OmpX
output:
*stick to specific surface (plastic, polystyrene, etc)
*stick nonspecifically (noncovalently) to surface (mussel peptide in reduced DOPA form)
*crosslink and stick (covalently) to surface and each other (mussel peptide in oxidized quinone form)
output apps:
*microbial biofilms/membranes
*bacterial photo-lithography (light-induced glueing)
*population separations
*sensor readout

Revision as of 12:09, 14 June 2007

Estrogen Biosensor

  • some articles:
    • Bacterial biosensor of endocrine modulators [1]
    • A yeast sensor of ligand binding [2]
    • Biosensor for estrogen in water samples [3]


sticky project:

input:

  • temperature (cold shock / heat shock promoters)
  • light (either via light sensitive transcription/translation or photo-induced chemistry)
  • cell growth (stationary phase, log phase)
  • cell type (if growing different strains)
  • metal ion (metal sensitive promoter)
  • estrogen (ER binding domain)

parts:

  • mussel peptide fused to circularly permuted OmpX (surface display protein, see Rice07)
    • need tyrosine hydroxylase to convert tyrosine to L-DOPA (hydroxylation)? can also occur non-enzymatically (reversible)
    • need tyrosinase to convert L-DOPA to DOPA-quinone (oxidation)? can also occur non-enzymatically (reversible) -- heavy metal ions (like copper) can catalyze...
  • polystyrene peptide fused to OmpX

output:

  • stick to specific surface (plastic, polystyrene, etc)
  • stick nonspecifically (noncovalently) to surface (mussel peptide in reduced DOPA form)
  • crosslink and stick (covalently) to surface and each other (mussel peptide in oxidized quinone form)

output apps:

  • microbial biofilms/membranes
  • bacterial photo-lithography (light-induced glueing)
  • population separations
  • sensor readout
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