IGEM:MIT/2007/Updated Ideas: Difference between revisions
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==sticky project:== | ===sticky project:=== | ||
input: | input: | ||
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*population separations | *population separations | ||
*sensor readout | *sensor readout | ||
* "Enhanced Bioaccumulation of Heavy Metal Ions by Bacterial Cells Due to Surface Display of Short Metal Binding Peptides"[http://aem.asm.org/cgi/reprint/65/3/1092] Check out refs about bacteria that "bioprecipitate" heavy metal ions |
Revision as of 12:36, 14 June 2007
Estrogen Biosensor
- some articles:
sticky project:
input:
- temperature (cold shock / heat shock promoters)
- light (either via light sensitive transcription/translation or photo-induced chemistry)
- cell growth (stationary phase, log phase)
- cell type (if growing different strains)
- metal ion (metal sensitive promoter)
- estrogen (ER binding domain)
parts:
- mussel peptide fused to circularly permuted OmpX (surface display protein, see Rice07)
- need tyrosine hydroxylase to convert tyrosine to L-DOPA (hydroxylation)? can also occur non-enzymatically (reversible)
- need tyrosinase to convert L-DOPA to DOPA-quinone (oxidation)? can also occur non-enzymatically (reversible) -- heavy metal ions (like copper) can catalyze...
- polystyrene peptide fused to OmpX
output:
- stick to specific surface (plastic, polystyrene, etc)
- stick nonspecifically (noncovalently) to surface (mussel peptide in reduced DOPA form)
- crosslink and stick (covalently) to surface and each other (mussel peptide in oxidized quinone form)
output apps:
- microbial biofilms/membranes
- bacterial photo-lithography (light-induced glueing)
- population separations
- sensor readout
- "Enhanced Bioaccumulation of Heavy Metal Ions by Bacterial Cells Due to Surface Display of Short Metal Binding Peptides"[4] Check out refs about bacteria that "bioprecipitate" heavy metal ions