IGEM:MIT/2007/Updated Ideas: Difference between revisions
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*metal ion (metal sensitive promoter) | *metal ion (metal sensitive promoter) | ||
*estrogen (ER binding domain) | *estrogen (ER binding domain) | ||
*cre/lox recombination | |||
parts: | parts: | ||
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*sensor readout | *sensor readout | ||
* "Enhanced Bioaccumulation of Heavy Metal Ions by Bacterial Cells Due to Surface Display of Short Metal Binding Peptides"[http://aem.asm.org/cgi/reprint/65/3/1092] Check out refs about bacteria that "bioprecipitate" heavy metal ions | * "Enhanced Bioaccumulation of Heavy Metal Ions by Bacterial Cells Due to Surface Display of Short Metal Binding Peptides"[http://aem.asm.org/cgi/reprint/65/3/1092] Check out refs about bacteria that "bioprecipitate" heavy metal ions | ||
articles on heavy metal adhesion: | |||
*[http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=91149] | |||
*Some applications of bacterial glue (from news) | |||
[http://www.ars.usda.gov/is/pr/2004/040720.htm Fiber-Hungry Bacteria Could Form Natural "Bond" With Wood Industry (Jul 2004)] | |||
[http://medgadget.com/archives/2006/04/bacterial_glue.html Bacterial Glue Could Become Medical Adhesive (Apr 2006)] | |||
==Water Decontamination Application Example (Smart Filtration System)== | |||
*Forrest: | |||
**Water is collected from a river (or other source) into a filtration setup | |||
**Bacteria is added to the water | |||
**The bacteria bind to or take in metals or other pollutants (input 1: detection/uptake of pollutant) | |||
**Input causes bacteria to be able to bind to the filter material (output 1: stickiness to filter material) | |||
**The water is now pollutant-free (can be put back into the river or otherwise used, etc) | |||
**The filter can be cleaned by rinsing/soaking it with water while shining light on it (input 2: light) | |||
**This second input causes the bacteria to unbind from the filter material (output 2: loss of stickiness to filter material) | |||
*Bernice: | |||
**Bacteria lights up when on filter material (perhaps at a certain concentration) | |||
**When filter glows to a certain extent, we know it's time to change the filter | |||
**Forrest: could we just add dye to the bacteria to stain it a certain color? | |||
[http://www.osha.gov/SLTC/metalsheavy/index.html Toxic Metals: Arsenic, Beryllium, Cadmium, Hexavalent Chronium, Lead, Mercury] | |||
[http://pages.prodigy.net/naturedoctor/metals.html Toxic Metal Exposure] | |||
[http://www.epa.qld.gov.au/environmental_management/air/air_quality_monitoring/air_pollutants/organic_pollutants/ Organic pollutants: Benzene, toluene, xylene, formaldehyde] | |||
[http://en.wikipedia.org/wiki/Persistent_organic_pollutant Persistent organic pollutants (POPs)] | |||
[http://en.wikipedia.org/wiki/Charles_River#Pollution_and_remediation_efforts Charles River remediation efforts] | |||
[http://www.boston.com/news/local/massachusetts/articles/2005/04/24/group_eyes_lawsuit_over_charles_river_pollution/ Group eyes lawsuit over Charles River pollution] | |||
[http://www.sciencemag.org/cgi/content/abstract/178/4057/158 Organic Compounds in the Charles River] | |||
==Creating a Hypothetical Martian Ecosystem== | |||
*[http://findarticles.com/p/articles/mi_qa3957/is_200110/ai_n8958142 Using photosynthetic bacteria to make oxygen on Mars] | |||
*[http://www.abc.net.au/science/news/stories/2007/1930936.htm?enviro Fungus eats radiation for breakfast] | |||
*[http://environment.newscientist.com/article/dn11614-extremeliving-bacteria-has-genome-sequenced.html Syntrophus aciditrophicus (extreme bacteria) has had its genome sequenced] | |||
*[http://microbewiki.kenyon.edu/index.php/Geobacter Geobacter bacterium has environmental restorative capabilities] | |||
== Photoreceptor Bacteria== | |||
*[http://www.technologyreview.com/Biotech/18436/ E. Coli that derive their energy from light] | |||
*[[Media:liphardt.pdf]] |
Latest revision as of 09:32, 22 June 2007
Estrogen Biosensor
- some articles:
sticky project:
input:
- temperature (cold shock / heat shock promoters)
- light (either via light sensitive transcription/translation or photo-induced chemistry)
- cell growth (stationary phase, log phase)
- cell type (if growing different strains)
- metal ion (metal sensitive promoter)
- estrogen (ER binding domain)
- cre/lox recombination
parts:
- mussel peptide fused to circularly permuted OmpX (surface display protein, see Rice07)
- need tyrosine hydroxylase to convert tyrosine to L-DOPA (hydroxylation)? can also occur non-enzymatically (reversible)
- need tyrosinase to convert L-DOPA to DOPA-quinone (oxidation)? can also occur non-enzymatically (reversible) -- heavy metal ions (like copper) can catalyze...
- polystyrene peptide fused to OmpX
output:
- stick to specific surface (plastic, polystyrene, etc)
- stick nonspecifically (noncovalently) to surface (mussel peptide in reduced DOPA form)
- crosslink and stick (covalently) to surface and each other (mussel peptide in oxidized quinone form)
output apps:
- microbial biofilms/membranes
- bacterial photo-lithography (light-induced glueing)
- population separations
- sensor readout
- "Enhanced Bioaccumulation of Heavy Metal Ions by Bacterial Cells Due to Surface Display of Short Metal Binding Peptides"[4] Check out refs about bacteria that "bioprecipitate" heavy metal ions
articles on heavy metal adhesion:
- Some applications of bacterial glue (from news)
Fiber-Hungry Bacteria Could Form Natural "Bond" With Wood Industry (Jul 2004)
Bacterial Glue Could Become Medical Adhesive (Apr 2006)
Water Decontamination Application Example (Smart Filtration System)
- Forrest:
- Water is collected from a river (or other source) into a filtration setup
- Bacteria is added to the water
- The bacteria bind to or take in metals or other pollutants (input 1: detection/uptake of pollutant)
- Input causes bacteria to be able to bind to the filter material (output 1: stickiness to filter material)
- The water is now pollutant-free (can be put back into the river or otherwise used, etc)
- The filter can be cleaned by rinsing/soaking it with water while shining light on it (input 2: light)
- This second input causes the bacteria to unbind from the filter material (output 2: loss of stickiness to filter material)
- Bernice:
- Bacteria lights up when on filter material (perhaps at a certain concentration)
- When filter glows to a certain extent, we know it's time to change the filter
- Forrest: could we just add dye to the bacteria to stain it a certain color?
Toxic Metals: Arsenic, Beryllium, Cadmium, Hexavalent Chronium, Lead, Mercury
Organic pollutants: Benzene, toluene, xylene, formaldehyde
Persistent organic pollutants (POPs)
Charles River remediation efforts
Group eyes lawsuit over Charles River pollution
Organic Compounds in the Charles River