IGEM:MIT/2008/Notebook/Yogurt

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*Our goal is to engineer a common yogurt bacteria, Lactobacillus bulgaricus, so that it will express the 20aa peptide p1025. A [http://www.ncbi.nlm.nih.gov/pubmed/9920267?dopt=Abstract |clinical study] reports that p1025 is good for your teeth (p1025 reduces oral colonization of a Streptococcus mutans, a tooth-decaying bacterium).
*Our goal is to engineer a common yogurt bacteria, Lactobacillus bulgaricus, so that it will express the 20aa peptide p1025. A [http://www.ncbi.nlm.nih.gov/pubmed/9920267?dopt=Abstract |clinical study] reports that p1025 is good for your teeth (p1025 reduces oral colonization of a Streptococcus mutans, a tooth-decaying bacterium).
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*We will express P1050 in E. coli as an intermediate step to evaluate protein secretion and efficacy.
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** Stage 1: Construction of p1025 fusion peptide and expression of gene in E. coli. This is an intermediate step to evaluate gene function and protein secretion/efficacy.
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** Stage 2: Binding Assay - see if the p1025 produced by E.coli inhibits binding of S. mutants to HA beads.
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** Stage 3: Expression of p1025 in Lactobacillus.
==Notes==
==Notes==

Revision as of 16:26, 16 June 2008

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Description

  • Our goal is to engineer a common yogurt bacteria, Lactobacillus bulgaricus, so that it will express the 20aa peptide p1025. A |clinical study reports that p1025 is good for your teeth (p1025 reduces oral colonization of a Streptococcus mutans, a tooth-decaying bacterium).
    • Stage 1: Construction of p1025 fusion peptide and expression of gene in E. coli. This is an intermediate step to evaluate gene function and protein secretion/efficacy.
    • Stage 2: Binding Assay - see if the p1025 produced by E.coli inhibits binding of S. mutants to HA beads.
    • Stage 3: Expression of p1025 in Lactobacillus.

Notes

  • Group meetings every Friday at 4pm in 68-574

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