IGEM:MIT/2008/Notebook/Yogurt

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
Line 15: Line 15:
==Description==
==Description==
-
*Our goal is to engineer a common yogurt bacteria, Lactobacillus bulgaricus, so that it will express the 20aa peptide p1025. A [http://www.ncbi.nlm.nih.gov/pubmed/9920267?dopt=Abstract |clinical study] reports that p1025 is good for your teeth (p1025 reduces oral colonization of a Streptococcus mutans, a tooth-decaying bacterium).
+
*Our goal is to engineer a common yogurt bacteria, Lactobacillus bulgaricus, so that it will express the 20aa peptide p1025. A clinical study [http://www.ncbi.nlm.nih.gov/pubmed/9920267?dopt=Abstract (Kelly CG et al.; Nature Biotechnol. 1999)] reports that p1025 is good for your teeth (p1025 reduces oral colonization of a Streptococcus mutans, a tooth-decaying bacterium).
** Stage 1: Construction of p1025 fusion peptide and expression of gene in E. coli. This is an intermediate step to evaluate gene function and protein secretion/efficacy.
** Stage 1: Construction of p1025 fusion peptide and expression of gene in E. coli. This is an intermediate step to evaluate gene function and protein secretion/efficacy.

Revision as of 15:33, 16 June 2008

Search this project

Customize your entry pages

Description

  • Our goal is to engineer a common yogurt bacteria, Lactobacillus bulgaricus, so that it will express the 20aa peptide p1025. A clinical study (Kelly CG et al.; Nature Biotechnol. 1999) reports that p1025 is good for your teeth (p1025 reduces oral colonization of a Streptococcus mutans, a tooth-decaying bacterium).
    • Stage 1: Construction of p1025 fusion peptide and expression of gene in E. coli. This is an intermediate step to evaluate gene function and protein secretion/efficacy.
    • Stage 2: Binding Assay - see if the p1025 produced by E.coli inhibits binding of S. mutants to HA beads.
    • Stage 3: Expression of p1025 in Lactobacillus.

Notes

  • Group meetings every Friday at 4pm in 68-574

Information

Useful Links





Personal tools