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* [[../Yogurt/lbulgaricus |''Lactobacillus delbrueckii'' subsp. ''bulgaricus'' Information & Protocols]]
* [[../Yogurt/lbulgaricus |''Lactobacillus delbrueckii'' subsp. ''bulgaricus'' Information & Protocols]]
* [[../Yogurt/toothbindingassay | Tooth binding assay protocol]]
==Useful Links==
==Useful Links==

Revision as of 15:10, 18 June 2008

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  • Our goal is to engineer a common yogurt bacteria, Lactobacillus bulgaricus, so that it will express the 20aa peptide p1025. A clinical study (Kelly CG et al.; Nature Biotechnol. 1999) reports that p1025 is good for your teeth. p1025 reduces oral colonization of Streptococcus mutans, a tooth-decaying bacterium.
    • Stage 1: Construction of p1025 fusion peptide and expression of gene in E. coli. This is an intermediate step to evaluate gene function and protein secretion/efficacy.
    • Stage 2: Binding Assay - see if the p1025 produced by E.coli inhibits binding of S. mutants to hydroxyapatite (HA) beads.
    • Stage 3: Expression of p1025 in Lactobacillus.
    • Stage 4: Make yogurt with modified Lactobacillus and test for inhibition of S. mutants binding.


  • Group meetings every Friday at 4pm in 68-474 (except 7/11 and 8/1 in 68-674)
  • Journal club meetings as needed every Friday at 11am in 68-474


Useful Links

  • |DNA Plasmid Editor
  • |Peptide Sequence Manipulations
  • Primer Analyzer Tool (check temperature & GC content) | One | Two
  • PMID 9920267
    A synthetic peptide adhesion epitope as a novel antimicrobial agent.
  • PMID 9062560
    Inhibition of Streptococcus mutans adsorption to hydroxyapatite by low-molecular-weight chitosans.
  • PMID 12788739
    (a secretion signal sequence described; can use the promoter, RBS and secretion signal for this protein for p1025).
  • PMID 11772607
    Electrotransformation of Lactobacillus delbrueckii subsp. bulgaricus and L. delbrueckii subsp. lactis with various plasmids.

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