IGEM:MIT/2008/Notebook/Yogurt

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*[http://slam.bs.jhmi.edu/gd/ |Peptide Sequence Manipulations]
*[http://slam.bs.jhmi.edu/gd/ |Peptide Sequence Manipulations]
*Primer Analyzer Tool (check temperature & GC content) [http://www.idtdna.com/analyzer/Applications/OligoAnalyzer/Default.aspx | One] [http://www.idtdna.com/analyzer/Applications/OligoAnalyzer/Default.aspx | Two]
*Primer Analyzer Tool (check temperature & GC content) [http://www.idtdna.com/analyzer/Applications/OligoAnalyzer/Default.aspx | One] [http://www.idtdna.com/analyzer/Applications/OligoAnalyzer/Default.aspx | Two]
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*PMID 9920267<br style="clear:both;"/>
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A synthetic peptide adhesion epitope as a novel antimicrobial agent.<br style="clear:both;"/>
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*PMID 12536251<br style="clear:both;"/>
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Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems.<br style="clear:both;"/>

Revision as of 08:45, 17 June 2008

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Description

  • Our goal is to engineer a common yogurt bacteria, Lactobacillus bulgaricus, so that it will express the 20aa peptide p1025. A clinical study (Kelly CG et al.; Nature Biotechnol. 1999) reports that p1025 is good for your teeth. p1025 reduces oral colonization of Streptococcus mutans, a tooth-decaying bacterium.
    • Stage 1: Construction of p1025 fusion peptide and expression of gene in E. coli. This is an intermediate step to evaluate gene function and protein secretion/efficacy.
    • Stage 2: Binding Assay - see if the p1025 produced by E.coli inhibits binding of S. mutants to hydroxyapatite (HA) beads.
    • Stage 3: Expression of p1025 in Lactobacillus.
    • Stage 4: Make yogurt with modified Lactobacillus and test for inhibition of S. mutants binding.

Notes

  • Group meetings every Friday at 4pm in 68-574

Information

Useful Links

A synthetic peptide adhesion epitope as a novel antimicrobial agent.

Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems.




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