IGEM:MIT/2008/Notebook/Yogurt

Description

• Our goal is to engineer a common yogurt bacteria, Lactobacillus bulgaricus, so that it will express the 20aa peptide p1025. A clinical study (Kelly CG et al.; Nature Biotechnol. 1999) reports that p1025 is good for your teeth (p1025 reduces oral colonization of a Streptococcus mutans, a tooth-decaying bacterium).

• • Stage 1: Construction of p1025 fusion peptide and expression of gene in E. coli. This is an intermediate step to evaluate gene function and protein secretion/efficacy.
  • Stage 2: Binding Assay - see if the p1025 produced by E.coli inhibits binding of S. mutants to HA beads.
  • Stage 3: Expression of p1025 in Lactobacillus.

Notes

• Group meetings every Friday at 4pm in 68-574

Information

• Brainstorming

• Daily Notebook

• Primers/Plasmids/PCR sequences

• Labeling system

Useful Links

• |DNA Plasmid Editor
• |Peptide Sequence Manipulations
• Primer Analyzer Tool (check temperature & GC content) | One | Two