IGEM:MIT/2008/Notebook/Yogurt/Notes: Difference between revisions
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| Line 18: | Line 18: | ||
*potential eptiopes | *potential eptiopes | ||
**FLAG (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) | **FLAG (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) | ||
**C-myc ( | **C-myc (E Q K L I S E E D L) | ||
** (- _ + _ _ _ - - - +) | ** (- _ + _ _ _ - - - +) | ||
**HA ( | **HA (Y P Y D U P D Y A: #2) | ||
** (_ _ _ - _ _ - _ _) | ** (_ _ _ - _ _ - _ _) | ||
Revision as of 08:48, 16 June 2008
JUN 10
JUN 16
Summary of individual tasks to research:
- S. Mutans metabolism pathway-Asad
- L. bulgaricus metabolism pathway-Allin
- secretion (full or partial)-John
- other secretion systems (introduction of)-Sara
- T7 as a promoter (L. bulgaricus)-Prarthna
- L. bulgaricus transformation/protocols-Derek
- binding assay protocols-Andrew
- epitope sequence
Linear Sequence/Design
Ex-promoter-RBS-ATG-signal-epitope-p1025-linker (TEV cleavage site)-GFP-His-TAATAA-termination-SP (see attached link for image of linear sequence)
- Base pairs: 35-10-3-90-30-60-45-700-15-6-8
- potential eptiopes
- FLAG (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys)
- C-myc (E Q K L I S E E D L)
- (- _ + _ _ _ - - - +)
- HA (Y P Y D U P D Y A: #2)
- (_ _ _ - _ _ - _ _)
