IGEM:MIT/2008/Notebook/Yogurt/Notes

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(Linear Sequence/Design)
(Parts/Construction)
Line 37: Line 37:
#L. bulgaricus
#L. bulgaricus
#S. Mutans
#S. Mutans
 +
===To Do/To Buy:===
 +
*finalize DNA sequence
 +
*primer
 +
*synthesis
 +
*reagents

Revision as of 13:46, 16 June 2008

Contents

JUN 10

Image:System_diagram_pic.jpg

JUN 16

Summary of individual tasks to research:

  1. S. Mutans metabolism pathway-Asad
  2. L. bulgaricus metabolism pathway-Allin
  3. secretion (full or partial)-John
  4. other secretion systems (introduction of)-Sara
  5. T7 as a promoter (L. bulgaricus)-Prarthna
  6. L. bulgaricus transformation/protocols-Derek
  7. binding assay protocols-Andrew
  8. epitope sequence

Linear Sequence/Design

Ex-promoter-RBS-ATG-signal-epitope-p1025-linker (TEV cleavage site)-GFP-His-TAATAA-termination-SP (see top of page)

  • Base pairs: 35-10-3-90-30-60-45-700-15-6-8
  • potential eptiopes
    • FLAG (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys)
    • C-myc (E Q K L I S E E D L)
      • (- _ + _ _ _ - - - +)
    • HA (Y P Y D U P D Y A: #2)
      • (_ _ _ - _ _ - _ _)

DNA/Plasmid Editor

Peptide Sequence Manipulations

Parts/Construction

  1. Construction
    1. GFP
    2. Synthetic peptide
    3. L. bulgaricus vector
  2. Assay
  3. L. bulgaricus
  4. S. Mutans

To Do/To Buy:

  • finalize DNA sequence
  • primer
  • synthesis
  • reagents
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