IGEM:MIT/2008/Notebook/Yogurt/sequences: Difference between revisions
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Revision as of 07:45, 18 June 2008
Primers
Include sequence, usage, temperatures, concentration, start date.
P1: Forward Primer for Promoter
- foward sequence: CCG CTT CTA GAG TAA TAC GAC TCA CTA TAG GGA ATA CAA GCT ACT TGT TCT TTT TGC ATA CTA GAG ATT AAA GAG GAG AAA TAC TAG ATG CAG AAG AAA AAA TCC GC
- length = 107 bp
- melting temp = 68.9 C
- GC content % = 37.4
P2: Reverse Primer for Peptide
- reverse sequence: GTT CTT CTC CTT TAC GCA TGC TGC CCC CGC CGC TGC CGC C
- length = 40 bp
- melting temp = 74.7 C
- GC content % = 67.5
TEV Protease cut site
- Amino Acid Sequence : Glu-Asn-Leu-Tyr-Phe-Gln-Gly (Cuts between Gln-Gly)
- Base Pair Sequence: GAAAACCTGTACTTCCAGGGT
P3: Forward Primer for GFP
- forward sequence: GGC GGC AGC GGC GGG GGC AGC ATG CGT AAA GGA GAA GAA C
- length = 40 bp
- melting temp = 74.7 C
- GC content % = 67.5
P4: Reverse Primer for GFP
- reverse sequence: CTG CAG CGG CCG CTA CTA GTA AGA GAA TAT AAA AAG CCA GAT TAT TAA TCC GGC TTT TTT ATT ATT TTT ATT AGT GGT GAT GGT GAT GAT GTT TGT ATA GTT CAT CCA TGC
- length = 111 bp
- melting temp = 69.6 C
- GC content % = 36.0
P5: Reverse Primer for GFP without Terminator
- reverse sequence: CTG CAG CGG CCG CTA CTA GTA TTA TTA GTG GTG ATG GTG ATG ATG TTT GTA TAG TTC ATC CAT GC
- length = 65bp
- GC content = 44.6 %
- melting temp = 68.5 ºC
Plasmids
Include sequence, BioBrick #, functional features, digestion map.
PCR Products
Include sequence, template and primers, functional features.
Other
DNA sequence to synthesize - epitope/p1025/linker (6/17/08)
- The signal peptide sequence can be found at the NCBI genome database
- 6/17, updated signal peptide, silent mutations in 1st part of linker (GGC instead of GGT coding for Gly)
sequence: ATGCAGAAGAAAAAATCCGCACGCCATTTGAACAAAGTGGCTGAA TTAGCCGCAGCACTGCTCCTATCAGCGAGTCCACTGGCGGGAACTTTC GACTACAAAGACGACGACGACAAAGGTGGTGGTCAGCTGAAAACCGCT GACCTGCCGGCTGGTCGTGACGAAACCACCTCTTTCGTTCTGGTTGGT GGTGGTTACCCGTACGACGTTCCGGACTACGCTGGCGGCGGCTCTGGC GGCGGCTCTGGCGGCGGCTCTGAAAACCTGTACTTCCAGGGTGGTGGT GGTTCTGGTGGTGGTTCTGGTGGTGGTTCT
Whole Sequence
CCGCTTCTAGAGTAATACGACTCACTATAGGGAATACAAG CTACTTGTTCTTTTTGCATACTAGAGATTAAAGAGGAGAA ATACTAGATGCAGAAGAAAAAATCCGCACGCCATTTGAAC AAAGTGGCTGAATTAGCCGCAGCACTGCTCCTATCAGCGA GTCCACTGGCGGGAACTTTCCAGTCAGCCGCTTTTGTCCA AGCTGCCAGTCAAGAAACGGACTACAAAGACGACGACGAC AAAGGTGGTGGTCAGCTGAAAACCGCTGACCTGCCGGCTG GTCGTGACGAAACCACCTCTTTCGTTCTGGTTGGTGGTGG TTACCCGTACGACGTTCCGGACTACGCTGGtGGtGGtTCT GGtGGtGGtTCTGGtGGtGGtTCTGAAAACCTGTACTTCC AGGGTGGTGGTGGTTCTGGTGGcGGCagcGGcGGgGGCag cATGCGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCA ATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAAT TTTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGG AAAACTTACCCTTAAATTTATTTGCACTACTGGAAAACTA CCTGTTCCATGGCCAACACTTGTCACTACTTTCGGTTATG GTGTTCAATGCTTTGCGAGATACCCAGATCATATGAAACA GCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTA CAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACA AGACACGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGT TAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGAT GGAAACATTCTTGGACACAAATTGGAATACAACTATAACT CACACAATGTATACATCATGGCAGACAAACAAAAGAATGG AATCAAAGTTAACTTCAAAATTAGACACAACATTGAAGAT GGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTC CAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTA CCTGTCCACACAATCTGCCCTTTCGAAAGATCCCAACGAA AAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTG CTGGGATTACACATGGCATGGATGAACTATACAAACATCA TCACCATCACCACTAATAAAAATAATAAAAAAGCCGGATT AATAATCTGGCTTTTTATATTCTCTTACTAGTAGCGGCCG CTGCAGGATTA