IGEM:MIT/2009/DNA/PCBsynthesis: Difference between revisions

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pcyA - I15009 in the registry (750bp)
pcyA - I15009 in the registry (750bp)


atggccgtcactgatttaagtttgaccaattcttccctgatgcctacgttgaacccgatgattcaacagttggccctggcgatcgccgctagttggcaaagtttacccctcaagccctatcaattgccggaggatttgggctacgtagaaggccgcctggaaggggaaaagttagtgattgaaaatcggtgctaccaaacgccccagtttcgcaaaatgcatttggagttggccaaggtgggcaaagggttggatattctccactgtgtaatgtttcctgagcctttatacggtctacctttgtttggctgtgacattgtggccggccccggtggagtaagtgcggctattgcggatctatcccccacccaaagcgatcgccaattgcccgcagcgtaccaaaaatcattggcagagctaggccagccagaatttgagcaacaacgggaattgcccccctggggagaaatattttctgaatattgtttattcatccgtcccagcaatgtcactgaagaagaaagatttgtacaaagggtagtggactttttgcaaattcattgtcaccaatccatcgttgccgaacccttgtctgaagctcaaactttggagcaccgtcaggggcaaattcattactgccaacaacaacagaaaaatgataaaacccgtcgggtactggaaaaagcttttggggaagcttgggcggaacggtatatgagccaagtcttatttgatgttatccaataataa
ATGGCCGTCACTGATTTAAGTTTGACCAATTCTTCCCTGATGCCTACGTTGAACCCGATGATTCAACAGT
TGGCCCTGGCGATCGCCGCTAGTTGGCAAAGTTTACCCCTCAAGCCCTATCAATTGCCGGAGGATTTGGG
CTACGTAGAAGGCCGCCTGGAAGGGGAAAAGTTAGTGATTGAAAATCGGTGCTACCAAACGCCCCAGTTT
CGCAAAATGCATTTGGAGTTGGCCAAGGTGGGCAAAGGGTTGGATATTCTCCACTGTGTAATGTTTCCTG
AGCCTTTATACGGTCTACCTTTGTTTGGCTGTGACATTGTGGCCGGCCCCGGTGGAGTAAGTGCGGCTAT
TGCGGATCTATCCCCCACCCAAAGCGATCGCCAATTGCCCGCAGCGTACCAAAAATCATTGGCAGAGCTA
GGCCAGCCAGAATTTGAGCAACAACGGGAATTGCCCCCCTGGGGAGAAATATTTTCTGAATATTGTTTAT
TCATCCGTCCCAGCAATGTCACTGAAGAAGAAAGATTTGTACAAAGGGTAGTGGACTTTTTGCAAATTCA
TTGTCACCAATCCATCGTTGCCGAACCCTTGTCTGAAGCTCAAACTTTGGAGCACCGTCAGGGGCAAATT
CATTACTGCCAACAACAACAGAAAAATGATAAAACCCGTCGGGTACTGGAAAAAGCTTTTGGGGAAGCTT
GGGCGGAACGGTATATGAGCCAAGTCTTATTTGATGTTATCCAATAATAA


[http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=retrieve&dopt=full_report&list_uids=820069&log$=genesensor3&logdbfrom=pubmed HY2] Sequence AT3G09150.2


HY2 Sequence AT3G09150.2
ATGGCTTTATCAATGGAGTTTGGGTTTTCAATTGGGTCATGCTTCAAGGCACCAAACCCACCTGTTCTAA
TCTCTGCAAGCCCTAATAAGATCAATTTCACGTTGAGAAGGAGAAAGAAAAGATTCTTACTTAGAGTCTC
TGCTGTGTCGTATAAGGAATTCGCAGAGTCTGCTTTAGAAGAAACCAGGAAAAGGATCGTTCTTGAACCT
TCACATCTCCAGGAAAAGTATAGTAGCATGACAGGACTAGATGGTAAGACCGAACTTCAAATGCTTGCTT
TTAAATCTTCAAAGATTAGACTCTTGAGGAGTATGGCAATAGAGAATGAGACAATGCAGGTCTTTGACTT
TGCGGGTTTCATGGAGCCTGAGTATGATACTCCCATATTCTGTGCTAACTTTTTCACATCTACCAACGTT
AACATAGTTGTATTGGACCTTAATCCTTTGCATCAGTTGACTGACCAGACGGATTACCAAGACAAGTATT
ATAACAAGATAATGTCCATATATCACAAATATGCTGAGACTTTCCCATGGGGAGGGAAATTGACTGGTGA
ATCCATAAAGTTTTTCTCGCCTTTGGTGATGTGGACTAGGTTTTCGTCTAGCAAAGAAAAACATAAGGCT
TTGTTCTCTGCGTTTCTAGAGTACTATCAGGCATGGCTTGAGATGACAATCCAAGTGAGGGAGGAGATGG
AACCATCTCATGTGAGAGCCAATTGTGAAGCACAACACAAGTACCTGACATGGCGAGCACAAAAGGATCC
TGGACATGGTCTTCTTAAAAGATTAGTAGGTGAAGCAAAGGCAAAGGAGCTGCTAAGGGATTTCCTGTTC
AATGGGGTGGATGAGTTAGGCACAAAAACATTCATTGATTACTTTCCAGAGTACCAAACAGAAGATGGAA
CTGTAAGCGATAAACGAAGTATCATTGGGAAGTCATATGAAACTCGTCCATGGGATTTAACAGGACAATT
TATCGGCTAA


<code>
===================================
  1 ATGGCTTTAT CAATGGAGTT TGGGTTTTCA ATTGGGTCAT GCTTCAAGGC
Oligos for XmaI-Kozak-ATG-MTS-SpeI
51 ACCAAACCCA CCTGTTCTAA TCTCTGCAAG CCCTAATAAG ATCAATTTCA
===================================
101 CGTTGAGAAG GAGAAAGAAA AGATTCTTAC TTAGAGTCTC TGCTGTGTCG
C CCGGG AACAAA ATG CAA CGC TCC ATT TTT GCG AGG TTC A CTAGT
151 TATAAGGAAT TCGCAGAGTC TGCTTTAGAA GAAACCAGGA AAAGGATCGT
GGGCC C TTGTTT TAC GTT GCG AGG TAA AAA CGC TCC AAG TGATC A
201 TCTTGAACCT TCACATCTCC AGGAAAAGTA TAGTAGCATG ACAGGACTAG
251 ATGGTAAGAC CGAACTTCAA ATGCTTGCTT TTAAATCTTC AAAGATTAGA
301 CTCTTGAGGA GTATGGCAAT AGAGAATGAG ACAATGCAGG TCTTTGACTT
351 TGCGGGTTTC ATGGAGCCTG AGTATGATAC TCCCATATTC TGTGCTAACT
401 TTTTCACATC TACCAACGTT AACATAGTTG TATTGGACCT TAATCCTTTG
451 CATCAGTTGA CTGACCAGAC GGATTACCAA GACAAGTATT ATAACAAGAT
501 AATGTCCATA TATCACAAAT ATGCTGAGAC TTTCCCATGG GGAGGGAAAT
551 TGACTGGTGA ATCCATAAAG TTTTTCTCGC CTTTGGTGAT GTGGACTAGG
601 TTTTCGTCTA GCAAAGAAAA ACATAAGGCT TTGTTCTCTG CGTTTCTAGA
651 GTACTATCAG GCATGGCTTG AGATGACAAT CCAAGTGAGG GAGGAGATGG
701 AACCATCTCA TGTGAGAGCC AATTGTGAAG CACAACACAA GTACCTGACA
751 TGGCGAGCAC AAAAGGATCC TGGACATGGT CTTCTTAAAA GATTAGTAGG
801 TGAAGCAAAG GCAAAGGAGC TGCTAAGGGA TTTCCTGTTC AATGGGGTGG
851 ATGAGTTAGG CACAAAAACA TTCATTGATT ACTTTCCAGA GTACCAAACA
901 GAAGATGGAA CTGTAAGCGA TAAACGAAGT ATCATTGGGA AGTCATATGA
951 AACTCGTCCA TGGGATTTAA CAGGACAATT TATCGGCTAA


</code>
 
 
XmaI_mts1(+), CCGGG AACAAA ATG CAA CGC TCC ATT TTT GCG AGG TTC A
 
 
SpeI_mts1(-), CTAGT GAA CCT CGC AAA AAT GGA GCG TTG CAT TTTGTT C

Latest revision as of 12:33, 12 June 2009

pcyA - I15009 in the registry (750bp)

ATGGCCGTCACTGATTTAAGTTTGACCAATTCTTCCCTGATGCCTACGTTGAACCCGATGATTCAACAGT TGGCCCTGGCGATCGCCGCTAGTTGGCAAAGTTTACCCCTCAAGCCCTATCAATTGCCGGAGGATTTGGG CTACGTAGAAGGCCGCCTGGAAGGGGAAAAGTTAGTGATTGAAAATCGGTGCTACCAAACGCCCCAGTTT CGCAAAATGCATTTGGAGTTGGCCAAGGTGGGCAAAGGGTTGGATATTCTCCACTGTGTAATGTTTCCTG AGCCTTTATACGGTCTACCTTTGTTTGGCTGTGACATTGTGGCCGGCCCCGGTGGAGTAAGTGCGGCTAT TGCGGATCTATCCCCCACCCAAAGCGATCGCCAATTGCCCGCAGCGTACCAAAAATCATTGGCAGAGCTA GGCCAGCCAGAATTTGAGCAACAACGGGAATTGCCCCCCTGGGGAGAAATATTTTCTGAATATTGTTTAT TCATCCGTCCCAGCAATGTCACTGAAGAAGAAAGATTTGTACAAAGGGTAGTGGACTTTTTGCAAATTCA TTGTCACCAATCCATCGTTGCCGAACCCTTGTCTGAAGCTCAAACTTTGGAGCACCGTCAGGGGCAAATT CATTACTGCCAACAACAACAGAAAAATGATAAAACCCGTCGGGTACTGGAAAAAGCTTTTGGGGAAGCTT GGGCGGAACGGTATATGAGCCAAGTCTTATTTGATGTTATCCAATAATAA

HY2 Sequence AT3G09150.2

ATGGCTTTATCAATGGAGTTTGGGTTTTCAATTGGGTCATGCTTCAAGGCACCAAACCCACCTGTTCTAA TCTCTGCAAGCCCTAATAAGATCAATTTCACGTTGAGAAGGAGAAAGAAAAGATTCTTACTTAGAGTCTC TGCTGTGTCGTATAAGGAATTCGCAGAGTCTGCTTTAGAAGAAACCAGGAAAAGGATCGTTCTTGAACCT TCACATCTCCAGGAAAAGTATAGTAGCATGACAGGACTAGATGGTAAGACCGAACTTCAAATGCTTGCTT TTAAATCTTCAAAGATTAGACTCTTGAGGAGTATGGCAATAGAGAATGAGACAATGCAGGTCTTTGACTT TGCGGGTTTCATGGAGCCTGAGTATGATACTCCCATATTCTGTGCTAACTTTTTCACATCTACCAACGTT AACATAGTTGTATTGGACCTTAATCCTTTGCATCAGTTGACTGACCAGACGGATTACCAAGACAAGTATT ATAACAAGATAATGTCCATATATCACAAATATGCTGAGACTTTCCCATGGGGAGGGAAATTGACTGGTGA ATCCATAAAGTTTTTCTCGCCTTTGGTGATGTGGACTAGGTTTTCGTCTAGCAAAGAAAAACATAAGGCT TTGTTCTCTGCGTTTCTAGAGTACTATCAGGCATGGCTTGAGATGACAATCCAAGTGAGGGAGGAGATGG AACCATCTCATGTGAGAGCCAATTGTGAAGCACAACACAAGTACCTGACATGGCGAGCACAAAAGGATCC TGGACATGGTCTTCTTAAAAGATTAGTAGGTGAAGCAAAGGCAAAGGAGCTGCTAAGGGATTTCCTGTTC AATGGGGTGGATGAGTTAGGCACAAAAACATTCATTGATTACTTTCCAGAGTACCAAACAGAAGATGGAA CTGTAAGCGATAAACGAAGTATCATTGGGAAGTCATATGAAACTCGTCCATGGGATTTAACAGGACAATT TATCGGCTAA

=======================

Oligos for XmaI-Kozak-ATG-MTS-SpeI

=======================

C CCGGG AACAAA ATG CAA CGC TCC ATT TTT GCG AGG TTC A CTAGT GGGCC C TTGTTT TAC GTT GCG AGG TAA AAA CGC TCC AAG TGATC A


XmaI_mts1(+), CCGGG AACAAA ATG CAA CGC TCC ATT TTT GCG AGG TTC A


SpeI_mts1(-), CTAGT GAA CCT CGC AAA AAT GGA GCG TTG CAT TTTGTT C

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