IGEM:MIT/2009/Primers: Difference between revisions

Revision as of 10:28, 24 June 2009

Location of Materials
Label	Date	Name	Location	Comment	Sequence
1-pcyA-MTS-f	6/8/2009	DM	??	GCG - Nde1 (RS) - MTS - PcyA	GCGCATATGCAACGCTCCATTTTTGCGAGGTTCGCCGTCACTGATTTAAGTTTGAC
2-pcyA-f	6/8/2009	DM	??	GCG - Nde1 (RS) - PcyA	GCGCATATGGCCGTCACTGATTTAAGTTTGAC
3-pcyA-b	6/8/2009	DM	??	GCG - Xba1 (RS) - PcyA	GCGTCTAGATTATTATTGGATAACATCAAATA
HY2-f + MTS	6/10/2009	BR	??	??	GCGGC CTCGAG AACAAA ATGCAACGCTCCATTTTTGCGAGGTTC GCTTTATCAATGGAGTTTGGGTTTTC
Mas70p	6/19/2009	AD	??	GCGGC - Asc1 - Mas70p hom	GCGGC GGCGCGCC AAGAGCTTCATTACAAGGAAC
Mas70p	6/19/2009	AD	??	GCGGC - Pac1 - Mas70p com	GCGGC TTAATTAA TGTTGCAATTGGTTGTAATAATAGTAG
Esc1	6/19/2009	AD	??	GCGGC - Asc1- Esc1 hom	GCGGC GGCGCGCC TCAAAAAAAAAAGAAACCTTTACTCC
Esc1	6/19/2009	AD	??	GCGGC - Pac1 - Esc1 com	GCGGC TTAATTAA TTATTTGTCAACACTTGGAGCGTATTCTG
Mps3	6/19/2009	AD	??	GCGGC - Asc1 - Mps3 hom	GCGGC GGCGCGCC AATAACTCAAATGAGCATAGGCGGG
Mps3	6/19/2009	AD	??	GCGGC - Pac1- Mps3 comp	GCGGC TTAATTAA TTCTCCCTCATTATTTTTCACGCTTGTC
Ras2	6/19/2009	AD	??	GCGGC - Asc1 - Ras2 hom	GCGGC GGCGCGCC TAGTAAGGCCGGTCAAGTTTCAAATGC
Ras 2	6/19/2009	AD	??	GCGGC - Pac1 - Ras2 comp	GCGGC TTAATTAA ACTTATAATACAACAGCCACCCGATCCG
Nyv1	6/19/2009	AD	??	GCGGC - Asc1 - Nv1 hom	GCGGC GGCGCGCC AAACGCTTTAATGGTATG
Snc1	6/19/2009	AD	??	GCGGC - Pac1 - Snc1 comp	GCGGC TTAATTAA TATTGGCACCCCTCTTAAAG	Snc1-Nyv1	6/19/2009	AD	??	Snc1 comp - Nyv1 comp	CTTCAATGGACGTTAATCT TATTCCTGCAGCCCACTTAG
Nyv1-Snc1	6/19/2009	AD	??	Nyv1 hom - Snc1 hom	CTAAGTGGGCTGCAGGAATA AGATTAACGTCCATTGAAG
Bcl2	6/19/2009	AD	??	Asc1- Bcl2 - Pac1	5' CGCGCC AAGACTTTGTTGUCTTTGGCTTTGGTTGGTGCTTGTATCACTTTGGGTGC TTACTTGGGTCACAAG TTAAT 3'

@@ Line 52: / Line 52: @@
 ! Nyv1-Snc1
 |6/19/2009 || AD || ?? || Nyv1 hom - Snc1 hom || CTAAGTGGGCTGCAGGAATA AGATTAACGTCCATTGAAG
+|-
+!Bcl2
+|6/19/2009 || AD || ?? || Asc1- Bcl2 - Pac1 ||5' CGCGCC AAGACTTTGTTGUCTTTGGCTTTGGTTGGTGCTTGTATCACTTTGGGTGC
+TTACTTGGGTCACAAG TTAAT 3'
 |}