IGEM:MIT/2009/Primers: Difference between revisions

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! HY2-f + MTS
! HY2-f + MTS
| 6/10/2009 || BR || ?? || ?? || GCGGC CTCGAG AACAAA ATGCAACGCTCCATTTTTGCGAGGTTC GCTTTATCAATGGAGTTTGGGTTTTC
| 6/10/2009 || BR || ?? || ?? || GCGGC CTCGAG AACAAA ATGCAACGCTCCATTTTTGCGAGGTTC GCTTTATCAATGGAGTTTGGGTTTTC
|-
! Mas70p
| 6/19/2009 || AD || ?? || GCGGC - Asc1 - Mas70p hom || GCGGC GGCGCGCC AAGAGCTTCATTACAAGGAAC
|-
! Mas70p
|6/19/2009 || AD || ?? || GCGGC - Pac1 - Mas70p com || GCGGC TTAATTAA TGTTGCAATTGGTTGTAATAATAGTAG
|-
! Esc1
| 6/19/2009 || AD || ?? || GCGGC - Asc1- Esc1 hom || GCGGC GGCGCGCC TCAAAAAAAAAAGAAACCTTTACTCC
|-
! Esc1
|6/19/2009 || AD || ?? || GCGGC - Pac1 - Esc1 com || GCGGC TTAATTAA TTATTTGTCAACACTTGGAGCGTATTCTG
|-
! Mps3
|6/19/2009 || AD || ?? || GCGGC - Asc1 - Mps3 hom ||GCGGC GGCGCGCC AATAACTCAAATGAGCATAGGCGGG
|-
! Mps3
|6/19/2009 || AD || ?? || GCGGC - Pac1- Mps3 comp || GCGGC TTAATTAA TTCTCCCTCATTATTTTTCACGCTTGTC
|-
! Ras2
| 6/19/2009 || AD || ?? || GCGGC - Asc1 - Ras2 hom || GCGGC GGCGCGCC TAGTAAGGCCGGTCAAGTTTCAAATGC
|-
! Ras 2
|6/19/2009 || AD || ?? || GCGGC - Pac1 - Ras2 comp || GCGGC TTAATTAA ACTTATAATACAACAGCCACCCGATCCG
|-
! Nyv1
| 6/19/2009 || AD || ?? || GCGGC - Asc1 - Nv1 hom || GCGGC GGCGCGCC AAACGCTTTAATGGTATG
|-
! Snc1
| 6/19/2009 || AD || ?? || GCGGC - Pac1 - Snc1 comp || GCGGC TTAATTAA TATTGGCACCCCTCTTAAAG
|-
! Snc1-Nyv1
|6/19/2009 || AD || ?? || Snc1 comp - Nyv1 comp || CTTCAATGGACGTTAATCT TATTCCTGCAGCCCACTTAG
|-
! Nyv1-Snc1
|6/19/2009 || AD || ?? || Nyv1 hom - Snc1 hom || CTAAGTGGGCTGCAGGAATA AGATTAACGTCCATTGAAG
|-
!Bcl2
|6/19/2009 || AD || ?? || Asc1- Bcl2 - Pac1 ||5' CGCGCC AAGACTTTGTTGUCTTTGGCTTTGGTTGGTGCTTGTATCACTTTGGGTGC
TTACTTGGGTCACAAG TTAAT 3'
|-
! Bcl2
|6/19/2009 || AD || ?? || Asc1- Bcl2 - Pac1 || 3' GG TTCTGAAACAACAGAAACCGAAACCAACCACGAACATAGTGAAACCCACGAATGAACCCAGTGTTC AAT 5'
|}
|}

Latest revision as of 11:36, 24 June 2009

Location of Materials
Label Date Name Location Comment Sequence
1-pcyA-MTS-f 6/8/2009 DM ?? GCG - Nde1 (RS) - MTS - PcyA GCGCATATGCAACGCTCCATTTTTGCGAGGTTCGCCGTCACTGATTTAAGTTTGAC
2-pcyA-f 6/8/2009 DM ?? GCG - Nde1 (RS) - PcyA GCGCATATGGCCGTCACTGATTTAAGTTTGAC
3-pcyA-b 6/8/2009 DM ?? GCG - Xba1 (RS) - PcyA GCGTCTAGATTATTATTGGATAACATCAAATA
HY2-f + MTS 6/10/2009 BR ?? ?? GCGGC CTCGAG AACAAA ATGCAACGCTCCATTTTTGCGAGGTTC GCTTTATCAATGGAGTTTGGGTTTTC
Mas70p 6/19/2009 AD ?? GCGGC - Asc1 - Mas70p hom GCGGC GGCGCGCC AAGAGCTTCATTACAAGGAAC
Mas70p 6/19/2009 AD ?? GCGGC - Pac1 - Mas70p com GCGGC TTAATTAA TGTTGCAATTGGTTGTAATAATAGTAG
Esc1 6/19/2009 AD ?? GCGGC - Asc1- Esc1 hom GCGGC GGCGCGCC TCAAAAAAAAAAGAAACCTTTACTCC
Esc1 6/19/2009 AD ?? GCGGC - Pac1 - Esc1 com GCGGC TTAATTAA TTATTTGTCAACACTTGGAGCGTATTCTG
Mps3 6/19/2009 AD ?? GCGGC - Asc1 - Mps3 hom GCGGC GGCGCGCC AATAACTCAAATGAGCATAGGCGGG
Mps3 6/19/2009 AD ?? GCGGC - Pac1- Mps3 comp GCGGC TTAATTAA TTCTCCCTCATTATTTTTCACGCTTGTC
Ras2 6/19/2009 AD ?? GCGGC - Asc1 - Ras2 hom GCGGC GGCGCGCC TAGTAAGGCCGGTCAAGTTTCAAATGC
Ras 2 6/19/2009 AD ?? GCGGC - Pac1 - Ras2 comp GCGGC TTAATTAA ACTTATAATACAACAGCCACCCGATCCG
Nyv1 6/19/2009 AD ?? GCGGC - Asc1 - Nv1 hom GCGGC GGCGCGCC AAACGCTTTAATGGTATG
Snc1 6/19/2009 AD ?? GCGGC - Pac1 - Snc1 comp GCGGC TTAATTAA TATTGGCACCCCTCTTAAAG
Snc1-Nyv1 6/19/2009 AD ?? Snc1 comp - Nyv1 comp CTTCAATGGACGTTAATCT TATTCCTGCAGCCCACTTAG
Nyv1-Snc1 6/19/2009 AD ?? Nyv1 hom - Snc1 hom CTAAGTGGGCTGCAGGAATA AGATTAACGTCCATTGAAG
Bcl2 6/19/2009 AD ?? Asc1- Bcl2 - Pac1 5' CGCGCC AAGACTTTGTTGUCTTTGGCTTTGGTTGGTGCTTGTATCACTTTGGGTGC

TTACTTGGGTCACAAG TTAAT 3'

Bcl2 6/19/2009 AD ?? Asc1- Bcl2 - Pac1 3' GG TTCTGAAACAACAGAAACCGAAACCAACCACGAACATAGTGAAACCCACGAATGAACCCAGTGTTC AAT 5'
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