IGEM:Melbourne/2008/BCRiboswitch/Existing Riboswitches: Difference between revisions
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'''''DNA Availability''''': ''Planning'' | '''''DNA Availability''''': ''Planning'' | ||
[[Sequence]] | [[/Sequence|Sequence]] | ||
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pTet: constitutively active promoter | pTet: constitutively active promoter | ||
[[Sequence]] | [[/Sequence|Sequence]] | ||
BBa_J01008 '''(Key)''' “BioBricked version of Isaacs' riboregulator trans-activating key, taR12” | BBa_J01008 '''(Key)''' “BioBricked version of Isaacs' riboregulator trans-activating key, taR12” | ||
[[Sequence]] | [[/Sequence|Sequence]] | ||
2. [http://parts.mit.edu/registry/index.php/Part:BBa_J01080 BBa_J01080]'''(Lock)''' | 2. [http://parts.mit.edu/registry/index.php/Part:BBa_J01080 BBa_J01080]'''(Lock)''' | ||
[[Sequence]] | [[/Sequence|Sequence]] | ||
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'''''DNA Availability''''': ''Planning'' | '''''DNA Availability''''': ''Planning'' | ||
[[Sequence]] | [[/Sequence|Sequence]] | ||
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'''''DNA Availability''''': ''Planning'' | '''''DNA Availability''''': ''Planning'' | ||
[[Sequence]] | [[/Sequence|Sequence]] | ||
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'''''DNA Availability''''': [http://parts.mit.edu/registry/index.php/Part:BBa_J23008 <font color="RED">Available!</font>] | '''''DNA Availability''''': [http://parts.mit.edu/registry/index.php/Part:BBa_J23008 <font color="RED">Available!</font>] | ||
[[Sequence]] | [[/Sequence|Sequence]] | ||
The region on J01086 that binds to the hairpin of J01080 anneals perfectly except for 3 base pairs which are mismatched. J23008 is exactly J01086 except these three base pairs have been corrected for perfect base pairing. | The region on J01086 that binds to the hairpin of J01080 anneals perfectly except for 3 base pairs which are mismatched. J23008 is exactly J01086 except these three base pairs have been corrected for perfect base pairing. | ||
Revision as of 05:22, 28 March 2008
From published articles
1. From Issacs et al (3), the crR12-taR12 pair produced the highest cis repression and greatest trans activation. It seems to have been very effective.
From Bioparts Registry
1. BBa_J01010 (Lock) “Biobricked version of Isaacs' riboregulator cis repressed lock, crR12”
DNA Availability: Planning
BBa_J01060 (Lock) = [pTet][J01010]
DNA Availability: Planning
pTet: constitutively active promoter
BBa_J01008 (Key) “BioBricked version of Isaacs' riboregulator trans-activating key, taR12”
2. BBa_J01080(Lock)
BBa_J01122 (Lock) [pTet][J01080][RFP][DblTerm]
DNA Availability: Planning
BBa_J01086 (Key)
DNA Availability: Planning
BBa_J23008 (Key) (slightly modified version of J01086)
DNA Availability: Available!
The region on J01086 that binds to the hairpin of J01080 anneals perfectly except for 3 base pairs which are mismatched. J23008 is exactly J01086 except these three base pairs have been corrected for perfect base pairing.
