IGEM:Melbourne/2008/BCRiboswitch/Existing Riboswitches: Difference between revisions
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[[IGEM:Melbourne/2008| | [[IGEM:Melbourne/2008|Melbourne Homepage]] | ||
[[IGEM:Melbourne/2008/BCRiboswitch|Return to Riboswitch Mainpage]] | [[IGEM:Melbourne/2008/BCRiboswitch|Return to Riboswitch Mainpage]] | ||
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== From Bioparts Registry == | == From Bioparts Registry == | ||
1 | ===1=== | ||
BBa_J01010 '''(Lock)''' “Biobricked version of Isaacs' riboregulator cis repressed lock, crR12” | |||
'''''DNA Availability''''': ''Planning'' | '''''DNA Availability''''': ''Planning'' | ||
[[Sequence]] | [[/Sequence#BBa_J01010|Sequence]] | ||
BBa_J01060 '''(Lock)''' = [pTet][ | BBa_J01060 '''(Lock)''' = [pTet][J01010] | ||
'''''DNA Availability''''': ''Planning'' | '''''DNA Availability''''': ''Planning'' | ||
| Line 23: | Line 24: | ||
pTet: constitutively active promoter | pTet: constitutively active promoter | ||
[[Sequence]] | [[/Sequence#BBa_J01060|Sequence]] | ||
BBa_J01008 '''(Key)''' “BioBricked version of Isaacs' riboregulator trans-activating key, taR12” | BBa_J01008 '''(Key)''' “BioBricked version of Isaacs' riboregulator trans-activating key, taR12” | ||
[[Sequence]] | [[/Sequence#BBa_J01008|Sequence]] | ||
===2=== | |||
[http://partsregistry.org/Part:BBa_J01080 BBa_J01080]'''(Lock)''' | |||
'''''DNA Availability''''': ''Planning'' | |||
[[/Sequence#BBa_J01080|Sequence]] | |||
BBa_J01122 '''(Lock)''' [pTet][J01080][RFP][DblTerm] ([http://parts.mit.edu/registry/index.php/Berkeley2006-RiboregulatorsMain Designed by Berkeley 06]) | |||
'''''DNA Availability''''': ''Planning'' | |||
[[/Sequence#BBa_J01122|Sequence]] | |||
BBa_J01086 '''(Key)''' | |||
'''''DNA Availability''''': ''Planning'' | |||
[[/Sequence#BBa_J01086|Sequence]] | |||
BBa_J23008 '''(Key)''' (slightly modified version of J01086) | |||
'''''DNA Availability''''': [http://partsregistry.org/Part:BBa_J23008 BBa_J23008 <font color="RED">Available!</font>] | |||
[[/Sequence#BBa_J23008|Sequence]] | |||
The region on J01086 that binds to the hairpin of J01080 anneals perfectly except for 3 base pairs which are mismatched. J23008 is exactly J01086 except these three base pairs have been corrected for perfect base pairing. | |||
===3=== | |||
BBa_J23066 '''(Key)''' ([http://parts.mit.edu/registry/index.php/Berkeley2006-RiboregulatorsMain Designed by Berkeley 06]) | |||
'''''DNA Availability''''': [http://partsregistry.org/Part:BBa_J23066 <font color="RED">Available!</font>] | |||
[http://partsregistry.org/Part:BBa_J23077 BBa_J23077] '''(Lock)''' [Ptet][J23078][RFP][TT] | |||
'''''DNA Availability''''': Planning | |||
===4=== | |||
BBa_J23070 '''(Key)''' ([http://parts.mit.edu/registry/index.php/Berkeley2006-RiboregulatorsMain Designed by Berkeley 06]) | |||
'''''DNA Availability''''': [http://partsregistry.org/Part:BBa_J23070 <font color="RED">Available!</font>] | |||
Latest revision as of 01:12, 8 May 2008
From published articles
1. From Issacs et al (3), the crR12-taR12 pair produced the highest cis repression and greatest trans activation. It seems to have been very effective.
From Bioparts Registry
1
BBa_J01010 (Lock) “Biobricked version of Isaacs' riboregulator cis repressed lock, crR12”
DNA Availability: Planning
BBa_J01060 (Lock) = [pTet][J01010]
DNA Availability: Planning
pTet: constitutively active promoter
BBa_J01008 (Key) “BioBricked version of Isaacs' riboregulator trans-activating key, taR12”
2
BBa_J01080(Lock)
DNA Availability: Planning
BBa_J01122 (Lock) [pTet][J01080][RFP][DblTerm] (Designed by Berkeley 06)
DNA Availability: Planning
BBa_J01086 (Key)
DNA Availability: Planning
BBa_J23008 (Key) (slightly modified version of J01086)
DNA Availability: BBa_J23008 Available!
The region on J01086 that binds to the hairpin of J01080 anneals perfectly except for 3 base pairs which are mismatched. J23008 is exactly J01086 except these three base pairs have been corrected for perfect base pairing.
3
BBa_J23066 (Key) (Designed by Berkeley 06)
DNA Availability: Available!
BBa_J23077 (Lock) [Ptet][J23078][RFP][TT]
DNA Availability: Planning
4
BBa_J23070 (Key) (Designed by Berkeley 06)
DNA Availability: Available!
