IGEM:Melbourne/2008/BCRiboswitch/Existing Riboswitches

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(From Bioparts Registry)
(From Bioparts Registry)
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The region on J01086 that binds to the hairpin of J01080 anneals perfectly except for 3 base pairs which are mismatched. J23008 is exactly J01086 except these three base pairs have been corrected for perfect base pairing.
The region on J01086 that binds to the hairpin of J01080 anneals perfectly except for 3 base pairs which are mismatched. J23008 is exactly J01086 except these three base pairs have been corrected for perfect base pairing.
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'''''DNA Availability''''': Planning
'''''DNA Availability''''': Planning
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Revision as of 01:37, 2 May 2008

Melbourne Homepage

Return to Riboswitch Mainpage


Contents

From published articles

1. From Issacs et al (3), the crR12-taR12 pair produced the highest cis repression and greatest trans activation. It seems to have been very effective.


From Bioparts Registry

1

BBa_J01010 (Lock) “Biobricked version of Isaacs' riboregulator cis repressed lock, crR12”

DNA Availability: Planning

Sequence


BBa_J01060 (Lock) = [pTet][J01010]

DNA Availability: Planning

pTet: constitutively active promoter

Sequence


BBa_J01008 (Key) “BioBricked version of Isaacs' riboregulator trans-activating key, taR12”

Sequence


2

BBa_J01080(Lock)

DNA Availability: Planning

Sequence


BBa_J01122 (Lock) [pTet][J01080][RFP][DblTerm] (Designed by Berkeley 06)

DNA Availability: Planning

Sequence


BBa_J01086 (Key)

DNA Availability: Planning

Sequence


BBa_J23008 (Key) (slightly modified version of J01086)

DNA Availability: Available!

Sequence

The region on J01086 that binds to the hairpin of J01080 anneals perfectly except for 3 base pairs which are mismatched. J23008 is exactly J01086 except these three base pairs have been corrected for perfect base pairing.


3

BBa_J23066 (Key) (Designed by Berkeley 06)

DNA Availability: Available!


BBa_J23077 (Lock) [Ptet][lock][RFP][TT]

DNA Availability: Planning


4

BBa_J23070 (Key) (Designed by Berkeley 06)

DNA Availability: Available!

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