IGEM:Melbourne/2008/BCRiboswitch/Existing Riboswitches
From published articles
1. From Issacs et al (3), the crR12-taR12 pair produced the highest cis repression and greatest trans activation. It seems to have been very effective.
From Bioparts Registry
1. BBa_J01010 (Lock) “Biobricked version of Isaacs' riboregulator cis repressed lock, crR12”
DNA Availability: Planning
BBa_J01060 (Lock) = [pTet][J01010]
DNA Availability: Planning
pTet: constitutively active promoter
BBa_J01008 (Key) “BioBricked version of Isaacs' riboregulator trans-activating key, taR12”
2. BBa_J01080(Lock)
BBa_J01122 (Lock) [pTet][J01080][RFP][DblTerm]
DNA Availability: Planning
BBa_J01086 (Key)
DNA Availability: Planning
BBa_J23008 (Key) (slightly modified version of J01086)
DNA Availability: Available!
The region on J01086 that binds to the hairpin of J01080 anneals perfectly except for 3 base pairs which are mismatched. J23008 is exactly J01086 except these three base pairs have been corrected for perfect base pairing.
