IGEM:Melbourne/2008/Concepts/Diagram legend: Difference between revisions
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(New page: Return to Melbourne Homepage ==Physical Components== ===Promoter=== thumb|left|A Promoter *DNA sequence that act as an upstream initiator for...) |
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*Form complex 3D shapes that affect the function of the protein | *Form complex 3D shapes that affect the function of the protein | ||
*While genes are confined to the nucleus of a cell, proteins can generally diffuse around and even out of the cell | *While genes are confined to the nucleus of a cell, proteins can generally diffuse around and even out of the cell | ||
*As always, the [http://en.wikipedia.org/wiki/Protein/ Wikipedia Entry] has far more information than I can give here | |||
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Latest revision as of 22:40, 21 December 2007
Physical Components
Promoter
- DNA sequence that act as an upstream initiator for transcription of a gene
- If a promoter is active, the downstream genes will be transcribed from DNA to RNA and the RNA can then be translated into proteins
- If a promoter is repressed, the downstream genes are not transcribed therefore no protein is produced
- Promoters are generally labelled with a P followed by a subscript that provides information about the promoter. For example, the Ptrc-2 promoter in the toggle switch is a fusion of promoters from the tryptophane (the tr part) and lac (the c part) genes
- For more info, see the Wikipedia entry
Gene
- DNA sequence that codes for a particular protein
- Is transcribed into RNA which is translated into a protein
- For our purposes, a gene is essentially a blueprint for a component of our system and through the promoter, we can choose when the component is produced
- Again, see the Wikipedia Entry for more detailed information if you want
Protein
- A string of amino acids whose sequence is determined by the gene code they were assembled from
- Form complex 3D shapes that affect the function of the protein
- While genes are confined to the nucleus of a cell, proteins can generally diffuse around and even out of the cell
- As always, the Wikipedia Entry has far more information than I can give here
Actions
Positive action
- Indicates that the action is a positive one
- Eg. This arrow from a gene to a protein shows that the gene produces the protein
- Eg. This arrow from a protein to a promoter shows that the protein interacts with the promoter to activate it, causing the genes downstream of the promoter to be expressed
Negative action
- Indicates that the action is a negative one ie. the first component is inhibiting the second component from acting somehow
- Eg. This arrow from a protein to a promoter means that the protein interacts with the promoter to prevent downstream genes from being expressed
- Eg. This arrow from a temperature to a protein means that at that temperature, the protein will not function