IGEM:Melbourne/2008/Notebook/BioBricks Used/Made: Difference between revisions
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| Line 12: | Line 12: | ||
! Antibiotic Resistance | ! Antibiotic Resistance | ||
! Size | ! Size | ||
! Sequencing Result | |||
|- | |- | ||
| P3 7F | | P3 7F | ||
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_J58102 J58102] | | [http://partsregistry.org/wiki/index.php?title=Part:BBa_J58102 J58102] | ||
| OmpC-RBS-GFP-Ter | | OmpC-RBS-GFP-Ter | ||
| Miniprepped, Glycerol stocked | | Miniprepped, Glycerol stocked, Sequenced | ||
| -20°C | | -20°C | ||
| Amp | | Amp | ||
| Line 24: | Line 25: | ||
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0083 R0083] | | [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0083 R0083] | ||
| OmpC mutated Promoter | | OmpC mutated Promoter | ||
| Miniprepped, Glycerol stocked | | Miniprepped, Glycerol stocked, Sequenced | ||
| -20°C | | -20°C | ||
| Amp | | Amp | ||
| Line 32: | Line 33: | ||
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_J58103 J58103] | | [http://partsregistry.org/wiki/index.php?title=Part:BBa_J58103 J58103] | ||
| OmpC(mut.)-RBS-GFP-Ter | | OmpC(mut.)-RBS-GFP-Ter | ||
| Miniprepped, Glycerol stocked | | Miniprepped, Glycerol stocked, Sequenced | ||
| -20°C | | -20°C | ||
| Amp | | Amp | ||
| Line 48: | Line 49: | ||
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_I13522 I13522] | | [http://partsregistry.org/wiki/index.php?title=Part:BBa_I13522 I13522] | ||
| pTetR-RBS-GFP-Ter | | pTetR-RBS-GFP-Ter | ||
| Miniprepped, Glycerol stocked | | Miniprepped, Glycerol stocked, Sequenced | ||
| -20°C | | -20°C | ||
| Amp | | Amp | ||
| 937bp | | 937bp | ||
| Good | |||
|- | |- | ||
| P0 4G | | P0 4G | ||
| Line 64: | Line 66: | ||
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_J23030 J23030] | | [http://partsregistry.org/wiki/index.php?title=Part:BBa_J23030 J23030] | ||
| Lock 3b | | Lock 3b | ||
| Miniprepped | | Miniprepped, Sequenced | ||
| -20 | | -20 | ||
| Amp | | Amp | ||
| 41bp | | 41bp | ||
| Good | |||
|- | |- | ||
| P0 1A | | P0 1A | ||
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0011 B0011] | | [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0011 B0011] | ||
| Terminator | | Terminator | ||
| Miniprepped | | Miniprepped, Sequenced | ||
| -20 | | -20 | ||
| Amp | | Amp | ||
| Line 80: | Line 83: | ||
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0012 B0012] | | [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0012 B0012] | ||
| Terminator | | Terminator | ||
| Miniprepped | | Miniprepped, Sequenced | ||
| -20 | | -20 | ||
| Amp | | Amp | ||
| Line 88: | Line 91: | ||
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0013 B0013] | | [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0013 B0013] | ||
| Terminator | | Terminator | ||
| Miniprepped | | Miniprepped, Sequenced | ||
| -20 | | -20 | ||
| Amp | | Amp | ||
| Line 96: | Line 99: | ||
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_J23032 J23032] | | [http://partsregistry.org/wiki/index.php?title=Part:BBa_J23032 J23032] | ||
| Lock 3d | | Lock 3d | ||
| Miniprepped | | Miniprepped, Sequenced | ||
| -20 | | -20 | ||
| Amp | | Amp | ||
| 43bp | | 43bp | ||
| Good | |||
|- | |- | ||
| P0 1H | | P0 1H | ||
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0024 B0024] | | [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0024 B0024] | ||
| double terminator | | double terminator | ||
| Miniprepped | | Miniprepped, Sequenced | ||
| -20 | | -20 | ||
| Amp | | Amp | ||
| 95 | | 95 | ||
| Good | |||
|- | |- | ||
| P2 8G | | P2 8G | ||
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_J23031 J23031] | | [http://partsregistry.org/wiki/index.php?title=Part:BBa_J23031 J23031] | ||
| lock 3C | | lock 3C | ||
| Miniprepped | | Miniprepped, Sequenced | ||
| -20 | | -20 | ||
| Amp | | Amp | ||
| 42bp | | 42bp | ||
| Bad | |||
|- | |- | ||
| P2 8B | | P2 8B | ||
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_J23007 J23007] | | [http://partsregistry.org/wiki/index.php?title=Part:BBa_J23007 J23007] | ||
| Key 3b | | Key 3b | ||
| Miniprepped, Glycerol stocked | | Miniprepped, Glycerol stocked, Sequenced | ||
| -20 | | -20 | ||
| Amp | | Amp | ||
| 27bp | | 27bp | ||
| Bad | |||
|- | |- | ||
| P2 8C | | P2 8C | ||
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_J23008 J23008] | | [http://partsregistry.org/wiki/index.php?title=Part:BBa_J23008 J23008] | ||
| Key 3c | | Key 3c | ||
| Miniprepped, Sequenced | | Miniprepped, Sequenced, Sequenced | ||
| -20 | | -20 | ||
| Amp | | Amp | ||
| 94bp | | 94bp | ||
| Good | |||
|- | |- | ||
| P2 8D | | P2 8D | ||
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_J23009 J23009] | | [http://partsregistry.org/wiki/index.php?title=Part:BBa_J23009 J23009] | ||
| Key 3d | | Key 3d | ||
| Miniprepped, Glycerol stocked | | Miniprepped, Glycerol stocked, Sequenced | ||
| -20 | | -20 | ||
| Amp | | Amp | ||
| 97bp | | 97bp | ||
| Bad | |||
|- | |- | ||
| P1 4B | | P1 4B | ||
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_E0240 E0240] | | [http://partsregistry.org/wiki/index.php?title=Part:BBa_E0240 E0240] | ||
| RBS-GFP-Ter | | RBS-GFP-Ter | ||
| Miniprepped, Glycerol stocked | | Miniprepped, Glycerol stocked, Sequenced | ||
| -20 | | -20 | ||
| Amp | | Amp | ||
| Line 192: | Line 201: | ||
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0040 C0040] | | [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0040 C0040] | ||
| tetracycline repressor from transposon Tn10 (+LVA) | | tetracycline repressor from transposon Tn10 (+LVA) | ||
| Miniprepped, Glycerol stocked | | Miniprepped, Glycerol stocked, Sequenced | ||
| -20 | | -20 | ||
| Amp | | Amp | ||
| Line 216: | Line 225: | ||
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 B0034] | | [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 B0034] | ||
| RBS (Elowitz 1999) -- defines RBS efficiency, based on Elowitz repressilator | | RBS (Elowitz 1999) -- defines RBS efficiency, based on Elowitz repressilator | ||
| Miniprepped, Glycerol stocked | | Miniprepped, Glycerol stocked, Sequenced | ||
| -20 | | -20 | ||
| Amp | | Amp | ||
| Line 224: | Line 233: | ||
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 B0015] | | [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 B0015] | ||
| double terminator (B0010-B0012) | | double terminator (B0010-B0012) | ||
| Miniprepped, Glycerol stocked | | Miniprepped, Glycerol stocked, Sequenced | ||
| -20 | | -20 | ||
| Amp, Kan | | Amp, Kan | ||
| Line 232: | Line 241: | ||
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0010 R0010] | | [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0010 R0010] | ||
| promoter (lacI regulated) | | promoter (lacI regulated) | ||
| Miniprepped, Glycerol stocked | | Miniprepped, Glycerol stocked, Sequenced | ||
| -20 | | -20 | ||
| Amp | | Amp | ||
| Line 240: | Line 249: | ||
| [http://partsregistry.org/Part:BBa_E0040 E0040] | | [http://partsregistry.org/Part:BBa_E0040 E0040] | ||
| GFP (no promoter, no RBS, no Ter) | | GFP (no promoter, no RBS, no Ter) | ||
| Miniprepped, Glycerol stocked | | Miniprepped, Glycerol stocked, Sequenced | ||
| -20 | | -20 | ||
| Amp | | Amp | ||
| 720 bp | | 720 bp | ||
| Bad | |||
|- | |- | ||
|} | |} | ||
Revision as of 01:02, 11 July 2008
BioBricks Used/Made
- Put info here of BioBricks taken from the kit
| Brick ID | BioBrick No. | Description | Condtion | Location | Antibiotic Resistance | Size | Sequencing Result |
|---|---|---|---|---|---|---|---|
| P3 7F | J58102 | OmpC-RBS-GFP-Ter | Miniprepped, Glycerol stocked, Sequenced | -20°C | Amp | 991bp | |
| P1 9G | R0083 | OmpC mutated Promoter | Miniprepped, Glycerol stocked, Sequenced | -20°C | Amp | 78bp | |
| P3 7G | J58103 | OmpC(mut.)-RBS-GFP-Ter | Miniprepped, Glycerol stocked, Sequenced | -20°C | Amp | 961bp | |
| ?? | I13504 | RBS-GFP-Ter | ?? | ?? | ?? | ?? | |
| P2 8A | I13522 | pTetR-RBS-GFP-Ter | Miniprepped, Glycerol stocked, Sequenced | -20°C | Amp | 937bp | Good |
| P0 4G | R0062 | Promoter (luxR & HSL regulated -- lux pR) | Miniprepped, Glycerol stocked | -20°C | Amp | 55bp | |
| P2 8F | J23030 | Lock 3b | Miniprepped, Sequenced | -20 | Amp | 41bp | Good |
| P0 1A | B0011 | Terminator | Miniprepped, Sequenced | -20 | Amp | 46 | |
| P0 1B | B0012 | Terminator | Miniprepped, Sequenced | -20 | Amp | 41 | |
| P0 1C | B0013 | Terminator | Miniprepped, Sequenced | -20 | Amp | 47 | |
| P2 8H | J23032 | Lock 3d | Miniprepped, Sequenced | -20 | Amp | 43bp | Good |
| P0 1H | B0024 | double terminator | Miniprepped, Sequenced | -20 | Amp | 95 | Good |
| P2 8G | J23031 | lock 3C | Miniprepped, Sequenced | -20 | Amp | 42bp | Bad |
| P2 8B | J23007 | Key 3b | Miniprepped, Glycerol stocked, Sequenced | -20 | Amp | 27bp | Bad |
| P2 8C | J23008 | Key 3c | Miniprepped, Sequenced, Sequenced | -20 | Amp | 94bp | Good |
| P2 8D | J23009 | Key 3d | Miniprepped, Glycerol stocked, Sequenced | -20 | Amp | 97bp | Bad |
| P1 4B | E0240 | RBS-GFP-Ter | Miniprepped, Glycerol stocked, Sequenced | -20 | Amp | 876bp | |
| P0 3E | C0061 | autoinducer synthetase for AHL | Miniprepped, Glycerol stocked | -20 | Amp | 618bp | |
| P3 8C | J36846 | Lpp-OmpA(46-159)-Streptavidin wild-type + His6 | Miniprepped, Glycerol stocked | -20 | Amp | 850bp | |
| P0 3F | C0062 | luxR repressor/activator, (no LVA?) | Miniprepped, Glycerol stocked | -20 | Amp | 756 bp | |
| P0 4C | R0040 | TetR repressible promoter | Miniprepped, Glycerol stocked | -20 | Amp | 54bp | |
| P1 12F | I13602 | Tet operator with CFP reporter (with LVA tag) [R/Tc+] | Miniprepped, Glycerol stocked | -20 | Amp | 979bp | |
| P0 2G | C0040 | tetracycline repressor from transposon Tn10 (+LVA) | Miniprepped, Glycerol stocked, Sequenced | -20 | Amp | 660 bp | |
| P13 5C | I0500 | Inducible pBad/araC | Miniprepped, Glycerol stocked | -20 | Kan | 1210bp | |
| P13 5E | R0073 | Promoter (Mnt regulated) | Miniprepped, Glycerol stocked | -20 | Kan | 67 bp | |
| P0 2E | B0034 | RBS (Elowitz 1999) -- defines RBS efficiency, based on Elowitz repressilator | Miniprepped, Glycerol stocked, Sequenced | -20 | Amp | 12 bp | |
| P16 5F | B0015 | double terminator (B0010-B0012) | Miniprepped, Glycerol stocked, Sequenced | -20 | Amp, Kan | 129 bp | |
| P2 5E | R0010 | promoter (lacI regulated) | Miniprepped, Glycerol stocked, Sequenced | -20 | Amp | 200 bp | |
| P1 7E | E0040 | GFP (no promoter, no RBS, no Ter) | Miniprepped, Glycerol stocked, Sequenced | -20 | Amp | 720 bp | Bad |
