IGEM:Melbourne/2008/Notebook/BioBricks Used/Made: Difference between revisions

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Line 12: Line 12:
! Antibiotic Resistance
! Antibiotic Resistance
! Size
! Size
! Sequencing Result
|-
|-
| P3 7F
| P3 7F
| J58102
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_J58102 J58102]
| OmpC-RBS-GFP-Ter
| OmpF-RBS-GFP-Ter
| Miniprepped
| Miniprepped, Glycerol stocked, Sequenced
| -20°C  
| -20°C  
| Amp
| Amp
Line 22: Line 23:
|-
|-
| P1 9G
| P1 9G
| R0083
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0083 R0083]
| OmpC mutated Promoter
| OmpF mutated Promoter
| Miniprepped
| Miniprepped, Glycerol stocked, Sequenced
| -20°C Red light box
| -20°C  
| Amp
| Amp
| 78bp
| 78bp
|-
|-
| P3 7G  
| P3 7G  
| J58103
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_J58103 J58103]
| OmpC(mut.)-RBS-GFP-Ter
| OmpF(mut.)-RBS-GFP-Ter
| Miniprepped
| Miniprepped, Glycerol stocked, Sequenced
| -20°C Organge box
| -20°C  
| Amp
| Amp
| 961bp
| 961bp
Line 40: Line 41:
| I13504
| I13504
| RBS-GFP-Ter
| RBS-GFP-Ter
| Miniprepped
| ??
| -20°C Red light box
| ??
| Amp
| ??
| ??
|-
|-
| P2 8A
| P2 8A
| I13522
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_I13522 I13522]
| pTetR-RBS-GFP-Ter
| pTetR-RBS-GFP-Ter
| Miniprepped
| Miniprepped, Glycerol stocked, Sequenced
| -20°C  
| -20°C  
| Amp
| Amp
| 937bp
| 937bp
| Good
|-
|-
| P0 4G
| P0 4G
| R0062
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0062 R0062]
| Promoter (luxR & HSL regulated -- lux pR)
| Promoter (luxR & HSL regulated -- lux pR)
| Miniprepped
| Miniprepped, Glycerol stocked
| -20°C
| -20°C
| Amp
| Amp
Line 61: Line 64:
|-
|-
| P2 8F
| P2 8F
| J23023
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_J23030 J23030]
| Lock 3b
| Lock 3b
| Miniprepped
| Miniprepped, Sequenced
| -20  
| -20  
| Amp
| Amp
| 41bp
| 41bp
| Good
|-
|-
| P0 1A
| P0 1A
| B0011
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0011 B0011]
| Terminator
| Terminator
| Miniprepped
| Miniprepped, Sequenced
| -20
| -20
| Amp
| Amp
| ??
| 46
|-
|-
| P0 1B
| P0 1B
| B0012
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0012 B0012]
| Terminator
| Terminator
| Miniprepped
| Miniprepped, Sequenced
| -20
| -20
| Amp
| Amp
| ??
| 41
|-
|-
| P0 1C
| P0 1C
| B0013
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0013 B0013]
| Terminator
| Terminator
| Miniprepped
| Miniprepped, Sequenced
| -20
| -20
| Amp
| Amp
| ??
| 47
|-
|-
| P2 8H
| P2 8H
| J23032
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_J23032 J23032]
| Lock 3d
| Lock 3d
| Miniprepped
| Miniprepped, Sequenced
| -20
| -20
| Amp
| Amp
| 43bp
| 43bp
| Good
|-
|-
| P0 1H
| P0 1H
| B0024
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0024 B0024]
| double terminator
| double terminator
| Miniprepped
| Miniprepped, Sequenced
| -20
| -20
| Amp
| Amp
| ??
| 95
| Good
|-
|-
| P2 8G
| P2 8G
| J23031
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_J23031 J23031]
| lock 3C
| lock 3C
| Miniprepped
| Miniprepped, Sequenced
| -20
| -20
| Amp
| Amp
| 42bp
| 42bp
|-
| Bad
| P1 8C
| E5500
| RBS-GFP
| Miniprepped
| -20
| Amp
| 741bp
|-
|-
| P2 8B
| P2 8B
| J23007
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_J23007 J23007]
| Key 3b
| Key 3b
| Miniprepped
| Miniprepped, Glycerol stocked, Sequenced
| -20
| -20
| Amp
| Amp
| 27bp
| 27bp
| Bad
|-
|-
| P2 8C
| P2 8C
| J23008
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_J23008 J23008]
| Key 3c
| Key 3c
| Miniprepped, Sequenced
| Miniprepped, Sequenced, Sequenced
| Where did it go???
| -20
| Amp
| Amp
| 94bp
| 94bp
| Good
|-
|-
| P2 8D
| P2 8D
| J23009
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_J23009 J23009]
| Key 3d
| Key 3d
| Miniprepped
| Miniprepped, Glycerol stocked, Sequenced
| -20
| -20
| Amp
| Amp
| 97bp
| 97bp
| Bad
|-
|-
| P1 4B
| P1 4B
| E0240
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_E0240 E0240]
| RBS-GFP-Ter
| RBS-GFP-Ter
| Miniprepped
| Miniprepped, Glycerol stocked, Sequenced
| -20
| -20
| Amp
| Amp
Line 157: Line 159:
|-
|-
| P0 3E
| P0 3E
| C0061
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0061 C0061]
| autoinducer synthetase for AHL  
| autoinducer synthetase for AHL  
| Miniprepped
| Miniprepped, Glycerol stocked
| -20
| -20
| Amp
| Amp
| 618bp
| 618bp
|-
| P0 4G
| R0062
| Promoter (luxR & HSL regulated -- lux pR)
| Miniprepped
| -20
| Amp
| 55bp
|-
|-
| P3 8C
| P3 8C
| J36846
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_J36846 J36846]
| Lpp-OmpA(46-159)-Streptavidin wild-type + His6  
| Lpp-OmpA(46-159)-Streptavidin wild-type + His6  
| Miniprepped
| Miniprepped, Glycerol stocked
| -20
| -20
| Amp
| Amp
Line 181: Line 175:
|-
|-
| P0 3F
| P0 3F
| C0062
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0062 C0062]
| luxR repressor/activator, (no LVA?)  
| luxR repressor/activator, (no LVA?)  
| Miniprepped
| Miniprepped, Glycerol stocked
| -20
| -20
| Amp
| Amp
Line 189: Line 183:
|-
|-
| P0 4C
| P0 4C
| R0040
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0040 R0040]
| TetR repressible promoter  
| TetR repressible promoter  
| Miniprepped
| Miniprepped, Glycerol stocked
| -20
| -20
| Amp
| Amp
Line 197: Line 191:
|-
|-
| P1 12F
| P1 12F
| I13602
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_I13602 I13602]
| Tet operator with CFP reporter (with LVA tag) [R/Tc+]  
| Tet operator with CFP reporter (with LVA tag) [R/Tc+]  
| Miniprepped, Glycerol stocked
| -20
| Amp
| 979bp
|-
| P0 2G
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0040 C0040]
| tetracycline repressor from transposon Tn10 (+LVA)
| Miniprepped, Glycerol stocked, Sequenced
| -20
| Amp
| 660 bp
|-
| P13 5C
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_I0500 I0500]
| Inducible pBad/araC
| Miniprepped, Glycerol stocked, Sequenced
| -20
| Kan
| 1210bp
| Bad
|-
| P13 5E
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0073 R0073]
| Promoter (Mnt regulated)
| Miniprepped, Glycerol stocked, Sequenced
| -20
| Kan
| 67 bp
| Bad
|-
| P0 2E
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 B0034]
| RBS (Elowitz 1999) -- defines RBS efficiency, based on Elowitz repressilator
| Miniprepped, Glycerol stocked, Sequenced
| -20
| Amp
| 12 bp
|-
| P16 5F
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 B0015]
| double terminator (B0010-B0012)
| Miniprepped, Glycerol stocked, Sequenced
| -20
| Amp, Kan
| 129 bp
|-
| P2 5E
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0010 R0010]
| promoter (lacI regulated)
| Miniprepped, Glycerol stocked, Sequenced
| -20
| Amp
| 200 bp
| Bad
|-
| P1 7E
| [http://partsregistry.org/Part:BBa_E0040 E0040]
| GFP-Ter-Ter
| Miniprepped, Glycerol stocked, Sequenced
| -20
| Amp
| 720 bp
| Bad
|-
| P6 3D
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_I763033 I763033]
| pTEtR-RBS-mRFP-Ter-Ter
| Miniprepped
| Miniprepped
| -20
| -20
| Amp
| Amp
| 979bp
| 923 bp
|-
| P2 9A
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_J23033 J23033]
| Lock 3e
| Miniprepped
| -20
| Amp
| 40 bp
|-
| P13 9H
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_I15009 I15009]
| phycocyanobilin, convert BV to PCB
| Miniprepped
| -20
| Kan
| 750 bp
|-
| P13 9G
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_I15008 I15008]
| heme oxygenase (ho1) from Synechocystis
| Miniprepped
| -20
| Kan
| 726bp
|-
| P0 3A
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0051 C0051]
| cI repressor from E. coli phage lambda (+LVA)
| Miniprepped, Glycerol stocked
| -20
| Amp
| 750bp
|-
| P0 5A
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0065 R0065]
| Promoter (lambda cI and luxR regulated -- hybrid) 
| Miniprepped, Glycerol stocked
| -20
| Amp
| 97bp
|-
| P14 8E
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_I759002 I759002]
| pTet-spacer-cro
| Miniprepped, Glycerol stocked
| -20
| Amp
| 312bp
|-
|-
 
| P8 1C
 
| [http://partsregistry.org/wiki/index.php?title=Part:pSB1A3 pSB1A3]
 
| High copy BioBrick assembly plasmid (AmpR)
 
| Miniprepped, Glycerol stocked
| -20
| Amp
| 2157bp
|}
|}

Latest revision as of 05:16, 28 July 2008

BioBricks Used/Made

  • Put info here of BioBricks taken from the kit
Brick ID BioBrick No. Description Condtion Location Antibiotic Resistance Size Sequencing Result
P3 7F J58102 OmpF-RBS-GFP-Ter Miniprepped, Glycerol stocked, Sequenced -20°C Amp 991bp
P1 9G R0083 OmpF mutated Promoter Miniprepped, Glycerol stocked, Sequenced -20°C Amp 78bp
P3 7G J58103 OmpF(mut.)-RBS-GFP-Ter Miniprepped, Glycerol stocked, Sequenced -20°C Amp 961bp
?? I13504 RBS-GFP-Ter ?? ?? ?? ??
P2 8A I13522 pTetR-RBS-GFP-Ter Miniprepped, Glycerol stocked, Sequenced -20°C Amp 937bp Good
P0 4G R0062 Promoter (luxR & HSL regulated -- lux pR) Miniprepped, Glycerol stocked -20°C Amp 55bp
P2 8F J23030 Lock 3b Miniprepped, Sequenced -20 Amp 41bp Good
P0 1A B0011 Terminator Miniprepped, Sequenced -20 Amp 46
P0 1B B0012 Terminator Miniprepped, Sequenced -20 Amp 41
P0 1C B0013 Terminator Miniprepped, Sequenced -20 Amp 47
P2 8H J23032 Lock 3d Miniprepped, Sequenced -20 Amp 43bp Good
P0 1H B0024 double terminator Miniprepped, Sequenced -20 Amp 95 Good
P2 8G J23031 lock 3C Miniprepped, Sequenced -20 Amp 42bp Bad
P2 8B J23007 Key 3b Miniprepped, Glycerol stocked, Sequenced -20 Amp 27bp Bad
P2 8C J23008 Key 3c Miniprepped, Sequenced, Sequenced -20 Amp 94bp Good
P2 8D J23009 Key 3d Miniprepped, Glycerol stocked, Sequenced -20 Amp 97bp Bad
P1 4B E0240 RBS-GFP-Ter Miniprepped, Glycerol stocked, Sequenced -20 Amp 876bp
P0 3E C0061 autoinducer synthetase for AHL Miniprepped, Glycerol stocked -20 Amp 618bp
P3 8C J36846 Lpp-OmpA(46-159)-Streptavidin wild-type + His6 Miniprepped, Glycerol stocked -20 Amp 850bp
P0 3F C0062 luxR repressor/activator, (no LVA?) Miniprepped, Glycerol stocked -20 Amp 756 bp
P0 4C R0040 TetR repressible promoter Miniprepped, Glycerol stocked -20 Amp 54bp
P1 12F I13602 Tet operator with CFP reporter (with LVA tag) [R/Tc+] Miniprepped, Glycerol stocked -20 Amp 979bp
P0 2G C0040 tetracycline repressor from transposon Tn10 (+LVA) Miniprepped, Glycerol stocked, Sequenced -20 Amp 660 bp
P13 5C I0500 Inducible pBad/araC Miniprepped, Glycerol stocked, Sequenced -20 Kan 1210bp Bad
P13 5E R0073 Promoter (Mnt regulated) Miniprepped, Glycerol stocked, Sequenced -20 Kan 67 bp Bad
P0 2E B0034 RBS (Elowitz 1999) -- defines RBS efficiency, based on Elowitz repressilator Miniprepped, Glycerol stocked, Sequenced -20 Amp 12 bp
P16 5F B0015 double terminator (B0010-B0012) Miniprepped, Glycerol stocked, Sequenced -20 Amp, Kan 129 bp
P2 5E R0010 promoter (lacI regulated) Miniprepped, Glycerol stocked, Sequenced -20 Amp 200 bp Bad
P1 7E E0040 GFP-Ter-Ter Miniprepped, Glycerol stocked, Sequenced -20 Amp 720 bp Bad
P6 3D I763033 pTEtR-RBS-mRFP-Ter-Ter Miniprepped -20 Amp 923 bp
P2 9A J23033 Lock 3e Miniprepped -20 Amp 40 bp
P13 9H I15009 phycocyanobilin, convert BV to PCB Miniprepped -20 Kan 750 bp
P13 9G I15008 heme oxygenase (ho1) from Synechocystis Miniprepped -20 Kan 726bp
P0 3A C0051 cI repressor from E. coli phage lambda (+LVA) Miniprepped, Glycerol stocked -20 Amp 750bp
P0 5A R0065 Promoter (lambda cI and luxR regulated -- hybrid) Miniprepped, Glycerol stocked -20 Amp 97bp
P14 8E I759002 pTet-spacer-cro Miniprepped, Glycerol stocked -20 Amp 312bp
P8 1C pSB1A3 High copy BioBrick assembly plasmid (AmpR) Miniprepped, Glycerol stocked -20 Amp 2157bp
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