IGEM:Melbourne/2008/Notebook/BioBricks Used/Made

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Line 13: Line 13:
! Size
! Size
|-
|-
-
 
+
| P3 7F
| J58102
| J58102
-
| OmpF-RBS-GFP-Ter
+
| OmpC-RBS-GFP-Ter
| Miniprepped
| Miniprepped
-
| -20°C Red light box
+
| -20°C  
-
| Amp
+
-
|-
+
-
 
+
-
| R0082
+
-
| OmpC Promoter
+
-
| Miniprepped
+
-
| -20°C Red light box
+
| Amp
| Amp
 +
| 991bp
|-
|-
| P1 9G
| P1 9G
Line 33: Line 27:
| -20°C Red light box
| -20°C Red light box
| Amp
| Amp
 +
| 78bp
|-
|-
| P3 7G  
| P3 7G  
Line 40: Line 35:
| -20°C Organge box
| -20°C Organge box
| Amp
| Amp
 +
| 961bp
|-
|-
Line 54: Line 50:
| -20°C  
| -20°C  
| Amp
| Amp
 +
| 937bp
|-
|-
| P0 4G
| P0 4G
Line 61: Line 58:
| -20°C
| -20°C
| Amp
| Amp
 +
| 55bp
|-
|-
-
|
+
| P2 8F
 +
| J23023
 +
| Lock 3b
 +
| Miniprepped
 +
| -20
 +
| Amp
 +
| 41bp
 +
|-
 +
| P0 1A
 +
| B0011
 +
| Terminator
 +
| Miniprepped
 +
| -20
 +
| Amp
 +
| ??
 +
|-
 +
| P0 1B
 +
| B0012
 +
| Terminator
 +
| Miniprepped
 +
| -20
 +
| Amp
 +
| ??
 +
|-
 +
| P0 1C
 +
| B0013
 +
| Terminator
 +
| Miniprepped
 +
| -20
 +
| Amp
 +
| ??
 +
|-
 +
| P2 8H
 +
| J23032
 +
| Lock 3d
 +
| Miniprepped
 +
| -20
 +
| Amp
 +
| 43bp
 +
|-
 +
| P0 1H
 +
| B0024
 +
| double terminator
 +
| Miniprepped
 +
| -20
 +
| Amp
 +
| ??
 +
|-
 +
| P2 8G
 +
| J23031
 +
| lock 3C
 +
| Miniprepped
 +
| -20
 +
| Amp
 +
| 42bp
 +
|-
 +
| P1 8C
 +
| E5500
 +
| RBS-GFP
 +
| Miniprepped
 +
| -20
 +
| Amp
 +
| 741bp
 +
|-
 +
| P2 8B
 +
| J23007
 +
| Key 3b
 +
| Miniprepped
 +
| -20
 +
| Amp
 +
| 27bp
 +
|-
 +
| P2 8C
 +
| J23008
 +
| Key 3c
 +
| Miniprepped, Sequenced
 +
| Where did it go???
 +
| Amp
 +
| 94bp
 +
|-
 +
| P2 8D
 +
| J23009
 +
| Key 3d
 +
| Miniprepped
 +
| -20
 +
| Amp
 +
| 97bp
 +
|-
 +
 
 +
 
|}
|}

Revision as of 10:01, 3 July 2008

BioBricks Used/Made

  • Put info here of BioBricks taken from the kit
Brick ID BioBrick No. Description Condtion Location Antibiotic Resistance Size
P3 7F J58102 OmpC-RBS-GFP-Ter Miniprepped -20°C Amp 991bp
P1 9G R0083 OmpC mutated Promoter Miniprepped -20°C Red light box Amp 78bp
P3 7G J58103 OmpC(mut.)-RBS-GFP-Ter Miniprepped -20°C Organge box Amp 961bp
I13504 RBS-GFP-Ter Miniprepped -20°C Red light box Amp
P2 8A I13522 pTetR-RBS-GFP-Ter Miniprepped -20°C Amp 937bp
P0 4G R0062 Promoter (luxR & HSL regulated -- lux pR) Miniprepped -20°C Amp 55bp
P2 8F J23023 Lock 3b Miniprepped -20 Amp 41bp
P0 1A B0011 Terminator Miniprepped -20 Amp  ??
P0 1B B0012 Terminator Miniprepped -20 Amp  ??
P0 1C B0013 Terminator Miniprepped -20 Amp  ??
P2 8H J23032 Lock 3d Miniprepped -20 Amp 43bp
P0 1H B0024 double terminator Miniprepped -20 Amp  ??
P2 8G J23031 lock 3C Miniprepped -20 Amp 42bp
P1 8C E5500 RBS-GFP Miniprepped -20 Amp 741bp
P2 8B J23007 Key 3b Miniprepped -20 Amp 27bp
P2 8C J23008 Key 3c Miniprepped, Sequenced Where did it go??? Amp 94bp
P2 8D J23009 Key 3d Miniprepped -20 Amp 97bp
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