IGEM:Melbourne/2008/Notebook/BioBricks Used/Made

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Line 37: Line 37:
| 961bp
| 961bp
|-
|-
-
 
+
| ??
| I13504
| I13504
| RBS-GFP-Ter
| RBS-GFP-Ter
Line 148: Line 148:
| 97bp
| 97bp
|-
|-
 +
| P1 4B
 +
| E0240
 +
| RBS-GFP-Ter
 +
| Miniprepped
 +
| -20
 +
| Amp
 +
| 876bp
 +
|-
 +
| P0 3E
 +
| C0061
 +
| autoinducer synthetase for AHL
 +
| Miniprepped
 +
| -20
 +
| Amp
 +
| 618bp
 +
|-
 +
| P0 4G
 +
| R0062
 +
| Promoter (luxR & HSL regulated -- lux pR)
 +
| Miniprepped
 +
| -20
 +
| Amp
 +
| 55bp
 +
|-
 +
| P3 8C
 +
| J36846
 +
| Lpp-OmpA(46-159)-Streptavidin wild-type + His6
 +
| Miniprepped
 +
| -20
 +
| Amp
 +
| 850bp
 +
|-
 +
| P0 3F
 +
| C0062
 +
| luxR repressor/activator, (no LVA?)
 +
| Miniprepped
 +
| -20
 +
| Amp
 +
| 756 bp
 +
|-
 +
| P0 4C
 +
| R0040
 +
| TetR repressible promoter
 +
| Miniprepped
 +
| -20
 +
| Amp
 +
| 54bp
 +
|-
 +
| P1 12F
 +
| I13602
 +
| Tet operator with CFP reporter (with LVA tag) [R/Tc+]
 +
| Miniprepped
 +
| -20
 +
| Amp
 +
| 979bp
 +
|-
 +
|}
|}

Revision as of 10:10, 3 July 2008

BioBricks Used/Made

  • Put info here of BioBricks taken from the kit
Brick ID BioBrick No. Description Condtion Location Antibiotic Resistance Size
P3 7F J58102 OmpC-RBS-GFP-Ter Miniprepped -20°C Amp 991bp
P1 9G R0083 OmpC mutated Promoter Miniprepped -20°C Red light box Amp 78bp
P3 7G J58103 OmpC(mut.)-RBS-GFP-Ter Miniprepped -20°C Organge box Amp 961bp
 ?? I13504 RBS-GFP-Ter Miniprepped -20°C Red light box Amp
P2 8A I13522 pTetR-RBS-GFP-Ter Miniprepped -20°C Amp 937bp
P0 4G R0062 Promoter (luxR & HSL regulated -- lux pR) Miniprepped -20°C Amp 55bp
P2 8F J23023 Lock 3b Miniprepped -20 Amp 41bp
P0 1A B0011 Terminator Miniprepped -20 Amp  ??
P0 1B B0012 Terminator Miniprepped -20 Amp  ??
P0 1C B0013 Terminator Miniprepped -20 Amp  ??
P2 8H J23032 Lock 3d Miniprepped -20 Amp 43bp
P0 1H B0024 double terminator Miniprepped -20 Amp  ??
P2 8G J23031 lock 3C Miniprepped -20 Amp 42bp
P1 8C E5500 RBS-GFP Miniprepped -20 Amp 741bp
P2 8B J23007 Key 3b Miniprepped -20 Amp 27bp
P2 8C J23008 Key 3c Miniprepped, Sequenced Where did it go??? Amp 94bp
P2 8D J23009 Key 3d Miniprepped -20 Amp 97bp
P1 4B E0240 RBS-GFP-Ter Miniprepped -20 Amp 876bp
P0 3E C0061 autoinducer synthetase for AHL Miniprepped -20 Amp 618bp
P0 4G R0062 Promoter (luxR & HSL regulated -- lux pR) Miniprepped -20 Amp 55bp
P3 8C J36846 Lpp-OmpA(46-159)-Streptavidin wild-type + His6 Miniprepped -20 Amp 850bp
P0 3F C0062 luxR repressor/activator, (no LVA?) Miniprepped -20 Amp 756 bp
P0 4C R0040 TetR repressible promoter Miniprepped -20 Amp 54bp
P1 12F I13602 Tet operator with CFP reporter (with LVA tag) [R/Tc+] Miniprepped -20 Amp 979bp
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