IGEM:Melbourne/2008/Notebook/BioBricks Used/Made

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Line 116: Line 116:
| Amp
| Amp
| 42bp
| 42bp
-
|-
 
-
| P1 8C
 
-
| E5500
 
-
| RBS-GFP
 
-
| Miniprepped
 
-
| -20
 
-
| Amp
 
-
| 741bp
 
|-
|-
| P2 8B
| P2 8B
Line 137: Line 129:
| Key 3c
| Key 3c
| Miniprepped, Sequenced
| Miniprepped, Sequenced
-
| Where did it go???
+
| -20
| Amp
| Amp
| 94bp
| 94bp

Revision as of 12:45, 4 July 2008

BioBricks Used/Made

  • Put info here of BioBricks taken from the kit
Brick ID BioBrick No. Description Condtion Location Antibiotic Resistance Size
P3 7F J58102 OmpC-RBS-GFP-Ter Miniprepped -20°C Amp 991bp
P1 9G R0083 OmpC mutated Promoter Miniprepped -20°C Amp 78bp
P3 7G J58103 OmpC(mut.)-RBS-GFP-Ter Miniprepped -20°C Amp 961bp
 ?? I13504 RBS-GFP-Ter  ??  ??  ??  ??
P2 8A I13522 pTetR-RBS-GFP-Ter Miniprepped -20°C Amp 937bp
P0 4G R0062 Promoter (luxR & HSL regulated -- lux pR) Miniprepped -20°C Amp 55bp
P2 8F J23023 Lock 3b Miniprepped -20 Amp 41bp
P0 1A B0011 Terminator Miniprepped -20 Amp  ??
P0 1B B0012 Terminator Miniprepped -20 Amp  ??
P0 1C B0013 Terminator Miniprepped -20 Amp  ??
P2 8H J23032 Lock 3d Miniprepped -20 Amp 43bp
P0 1H B0024 double terminator Miniprepped -20 Amp  ??
P2 8G J23031 lock 3C Miniprepped -20 Amp 42bp
P2 8B J23007 Key 3b Miniprepped -20 Amp 27bp
P2 8C J23008 Key 3c Miniprepped, Sequenced -20 Amp 94bp
P2 8D J23009 Key 3d Miniprepped -20 Amp 97bp
P1 4B E0240 RBS-GFP-Ter Miniprepped -20 Amp 876bp
P0 3E C0061 autoinducer synthetase for AHL Miniprepped -20 Amp 618bp
P0 4G R0062 Promoter (luxR & HSL regulated -- lux pR) Miniprepped -20 Amp 55bp
P3 8C J36846 Lpp-OmpA(46-159)-Streptavidin wild-type + His6 Miniprepped -20 Amp 850bp
P0 3F C0062 luxR repressor/activator, (no LVA?) Miniprepped -20 Amp 756 bp
P0 4C R0040 TetR repressible promoter Miniprepped -20 Amp 54bp
P1 12F I13602 Tet operator with CFP reporter (with LVA tag) [R/Tc+] Miniprepped -20 Amp 979bp
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