IGEM:Melbourne/2008/Notebook/BioBricks Used/Made

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Line 16: Line 16:
| J58102
| J58102
| OmpC-RBS-GFP-Ter
| OmpC-RBS-GFP-Ter
-
| Miniprepped
+
| Miniprepped, Glycerol stocked
| -20°C  
| -20°C  
| Amp
| Amp
Line 24: Line 24:
| R0083
| R0083
| OmpC mutated Promoter
| OmpC mutated Promoter
-
| Miniprepped
+
| Miniprepped, Glycerol stocked
| -20°C  
| -20°C  
| Amp
| Amp
Line 32: Line 32:
| J58103
| J58103
| OmpC(mut.)-RBS-GFP-Ter
| OmpC(mut.)-RBS-GFP-Ter
-
| Miniprepped
+
| Miniprepped, Glycerol stocked
| -20°C  
| -20°C  
| Amp
| Amp
Line 48: Line 48:
| I13522
| I13522
| pTetR-RBS-GFP-Ter
| pTetR-RBS-GFP-Ter
-
| Miniprepped
+
| Miniprepped, Glycerol stocked
| -20°C  
| -20°C  
| Amp
| Amp
Line 56: Line 56:
| R0062
| R0062
| Promoter (luxR & HSL regulated -- lux pR)
| Promoter (luxR & HSL regulated -- lux pR)
-
| Miniprepped
+
| Miniprepped, Glycerol stocked
| -20°C
| -20°C
| Amp
| Amp
Line 120: Line 120:
| J23007
| J23007
| Key 3b
| Key 3b
-
| Miniprepped
+
| Miniprepped, Glycerol stocked
| -20
| -20
| Amp
| Amp
Line 136: Line 136:
| J23009
| J23009
| Key 3d
| Key 3d
-
| Miniprepped
+
| Miniprepped, Glycerol stocked
| -20
| -20
| Amp
| Amp
Line 144: Line 144:
| E0240
| E0240
| RBS-GFP-Ter
| RBS-GFP-Ter
-
| Miniprepped
+
| Miniprepped, Glycerol stocked
| -20
| -20
| Amp
| Amp
Line 152: Line 152:
| C0061
| C0061
| autoinducer synthetase for AHL  
| autoinducer synthetase for AHL  
-
| Miniprepped
+
| Miniprepped, Glycerol stocked
| -20
| -20
| Amp
| Amp
Line 160: Line 160:
| R0062
| R0062
| Promoter (luxR & HSL regulated -- lux pR)  
| Promoter (luxR & HSL regulated -- lux pR)  
-
| Miniprepped
+
| Miniprepped, Glycerol stocked
| -20
| -20
| Amp
| Amp
Line 168: Line 168:
| J36846
| J36846
| Lpp-OmpA(46-159)-Streptavidin wild-type + His6  
| Lpp-OmpA(46-159)-Streptavidin wild-type + His6  
-
| Miniprepped
+
| Miniprepped, Glycerol stocked
| -20
| -20
| Amp
| Amp
Line 176: Line 176:
| C0062
| C0062
| luxR repressor/activator, (no LVA?)  
| luxR repressor/activator, (no LVA?)  
-
| Miniprepped
+
| Miniprepped, Glycerol stocked
| -20
| -20
| Amp
| Amp
Line 184: Line 184:
| R0040
| R0040
| TetR repressible promoter  
| TetR repressible promoter  
-
| Miniprepped
+
| Miniprepped, Glycerol stocked
| -20
| -20
| Amp
| Amp
Line 192: Line 192:
| I13602
| I13602
| Tet operator with CFP reporter (with LVA tag) [R/Tc+]  
| Tet operator with CFP reporter (with LVA tag) [R/Tc+]  
-
| Miniprepped
+
| Miniprepped, Glycerol stocked
| -20
| -20
| Amp
| Amp
| 979bp
| 979bp
|-
|-
-
 
+
| P0 2G
-
 
+
| C0040
-
 
+
| tetracycline repressor from transposon Tn10 (+LVA)
-
 
+
| Miniprepped, Glycerol stocked
 +
| -20
 +
| Amp
 +
| 660 bp
 +
|-
|}
|}

Revision as of 04:59, 5 July 2008

BioBricks Used/Made

  • Put info here of BioBricks taken from the kit
Brick ID BioBrick No. Description Condtion Location Antibiotic Resistance Size
P3 7F J58102 OmpC-RBS-GFP-Ter Miniprepped, Glycerol stocked -20°C Amp 991bp
P1 9G R0083 OmpC mutated Promoter Miniprepped, Glycerol stocked -20°C Amp 78bp
P3 7G J58103 OmpC(mut.)-RBS-GFP-Ter Miniprepped, Glycerol stocked -20°C Amp 961bp
 ?? I13504 RBS-GFP-Ter  ??  ??  ??  ??
P2 8A I13522 pTetR-RBS-GFP-Ter Miniprepped, Glycerol stocked -20°C Amp 937bp
P0 4G R0062 Promoter (luxR & HSL regulated -- lux pR) Miniprepped, Glycerol stocked -20°C Amp 55bp
P2 8F J23023 Lock 3b Miniprepped -20 Amp 41bp
P0 1A B0011 Terminator Miniprepped -20 Amp  ??
P0 1B B0012 Terminator Miniprepped -20 Amp  ??
P0 1C B0013 Terminator Miniprepped -20 Amp  ??
P2 8H J23032 Lock 3d Miniprepped -20 Amp 43bp
P0 1H B0024 double terminator Miniprepped -20 Amp  ??
P2 8G J23031 lock 3C Miniprepped -20 Amp 42bp
P2 8B J23007 Key 3b Miniprepped, Glycerol stocked -20 Amp 27bp
P2 8C J23008 Key 3c Miniprepped, Sequenced -20 Amp 94bp
P2 8D J23009 Key 3d Miniprepped, Glycerol stocked -20 Amp 97bp
P1 4B E0240 RBS-GFP-Ter Miniprepped, Glycerol stocked -20 Amp 876bp
P0 3E C0061 autoinducer synthetase for AHL Miniprepped, Glycerol stocked -20 Amp 618bp
P0 4G R0062 Promoter (luxR & HSL regulated -- lux pR) Miniprepped, Glycerol stocked -20 Amp 55bp
P3 8C J36846 Lpp-OmpA(46-159)-Streptavidin wild-type + His6 Miniprepped, Glycerol stocked -20 Amp 850bp
P0 3F C0062 luxR repressor/activator, (no LVA?) Miniprepped, Glycerol stocked -20 Amp 756 bp
P0 4C R0040 TetR repressible promoter Miniprepped, Glycerol stocked -20 Amp 54bp
P1 12F I13602 Tet operator with CFP reporter (with LVA tag) [R/Tc+] Miniprepped, Glycerol stocked -20 Amp 979bp
P0 2G C0040 tetracycline repressor from transposon Tn10 (+LVA) Miniprepped, Glycerol stocked -20 Amp 660 bp
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