IGEM:Melbourne/2008/Notebook/BioBricks Used/Made

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Line 14: Line 14:
|-
|-
| P3 7F
| P3 7F
-
| J58102
+
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_J58102 J58102]
| OmpC-RBS-GFP-Ter
| OmpC-RBS-GFP-Ter
| Miniprepped, Glycerol stocked
| Miniprepped, Glycerol stocked
Line 22: Line 22:
|-
|-
| P1 9G
| P1 9G
-
| R0083
+
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0083 R0083]
| OmpC mutated Promoter
| OmpC mutated Promoter
| Miniprepped, Glycerol stocked
| Miniprepped, Glycerol stocked
Line 30: Line 30:
|-
|-
| P3 7G  
| P3 7G  
-
| J58103
+
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_J58103 J58103]
| OmpC(mut.)-RBS-GFP-Ter
| OmpC(mut.)-RBS-GFP-Ter
| Miniprepped, Glycerol stocked
| Miniprepped, Glycerol stocked
Line 46: Line 46:
|-
|-
| P2 8A
| P2 8A
-
| I13522
+
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_I13522 I13522]
| pTetR-RBS-GFP-Ter
| pTetR-RBS-GFP-Ter
| Miniprepped, Glycerol stocked
| Miniprepped, Glycerol stocked
Line 54: Line 54:
|-
|-
| P0 4G
| P0 4G
-
| R0062
+
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0062 R0062]
| Promoter (luxR & HSL regulated -- lux pR)
| Promoter (luxR & HSL regulated -- lux pR)
| Miniprepped, Glycerol stocked
| Miniprepped, Glycerol stocked
Line 62: Line 62:
|-
|-
| P2 8F
| P2 8F
-
| J23023
+
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_J23023 J23023]
| Lock 3b
| Lock 3b
| Miniprepped
| Miniprepped
Line 70: Line 70:
|-
|-
| P0 1A
| P0 1A
-
| B0011
+
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0011 B0011]
| Terminator
| Terminator
| Miniprepped
| Miniprepped
Line 78: Line 78:
|-
|-
| P0 1B
| P0 1B
-
| B0012
+
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0012 B0012]
| Terminator
| Terminator
| Miniprepped
| Miniprepped
Line 86: Line 86:
|-
|-
| P0 1C
| P0 1C
-
| B0013
+
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0013 B0013]
| Terminator
| Terminator
| Miniprepped
| Miniprepped
Line 94: Line 94:
|-
|-
| P2 8H
| P2 8H
-
| J23032
+
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_J23032 J23032]
| Lock 3d
| Lock 3d
| Miniprepped
| Miniprepped
Line 102: Line 102:
|-
|-
| P0 1H
| P0 1H
-
| B0024
+
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0024 B0024]
| double terminator
| double terminator
| Miniprepped
| Miniprepped
Line 110: Line 110:
|-
|-
| P2 8G
| P2 8G
-
| J23031
+
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_J23031 J23031]
| lock 3C
| lock 3C
| Miniprepped
| Miniprepped
Line 118: Line 118:
|-
|-
| P2 8B
| P2 8B
-
| J23007
+
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_J23007 J23007]
| Key 3b
| Key 3b
| Miniprepped, Glycerol stocked
| Miniprepped, Glycerol stocked
Line 126: Line 126:
|-
|-
| P2 8C
| P2 8C
-
| J23008
+
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_J23008 J23008]
| Key 3c
| Key 3c
| Miniprepped, Sequenced
| Miniprepped, Sequenced
Line 134: Line 134:
|-
|-
| P2 8D
| P2 8D
-
| J23009
+
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_J23009 J23009]
| Key 3d
| Key 3d
| Miniprepped, Glycerol stocked
| Miniprepped, Glycerol stocked
Line 142: Line 142:
|-
|-
| P1 4B
| P1 4B
-
| E0240
+
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_E0240 E0240]
| RBS-GFP-Ter
| RBS-GFP-Ter
| Miniprepped, Glycerol stocked
| Miniprepped, Glycerol stocked
Line 150: Line 150:
|-
|-
| P0 3E
| P0 3E
-
| C0061
+
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0061 C0061]
| autoinducer synthetase for AHL  
| autoinducer synthetase for AHL  
| Miniprepped, Glycerol stocked
| Miniprepped, Glycerol stocked
Line 156: Line 156:
| Amp
| Amp
| 618bp
| 618bp
-
|-
 
-
| P0 4G
 
-
| R0062
 
-
| Promoter (luxR & HSL regulated -- lux pR)
 
-
| Miniprepped, Glycerol stocked
 
-
| -20
 
-
| Amp
 
-
| 55bp
 
|-
|-
| P3 8C
| P3 8C
-
| J36846
+
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_J36846 J36846]
| Lpp-OmpA(46-159)-Streptavidin wild-type + His6  
| Lpp-OmpA(46-159)-Streptavidin wild-type + His6  
| Miniprepped, Glycerol stocked
| Miniprepped, Glycerol stocked
Line 174: Line 166:
|-
|-
| P0 3F
| P0 3F
-
| C0062
+
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0062 C0062]
| luxR repressor/activator, (no LVA?)  
| luxR repressor/activator, (no LVA?)  
| Miniprepped, Glycerol stocked
| Miniprepped, Glycerol stocked
Line 182: Line 174:
|-
|-
| P0 4C
| P0 4C
-
| R0040
+
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0040 R0040]
-
| TetR repressible promoter
+
| Miniprepped, Glycerol stocked
| Miniprepped, Glycerol stocked
| -20
| -20
Line 190: Line 181:
|-
|-
| P1 12F
| P1 12F
-
| I13602
+
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_I13602 I13602]
| Tet operator with CFP reporter (with LVA tag) [R/Tc+]  
| Tet operator with CFP reporter (with LVA tag) [R/Tc+]  
| Miniprepped, Glycerol stocked
| Miniprepped, Glycerol stocked
Line 198: Line 189:
|-
|-
| P0 2G
| P0 2G
-
| C0040
+
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0040 C0040]
| tetracycline repressor from transposon Tn10 (+LVA)
| tetracycline repressor from transposon Tn10 (+LVA)
| Miniprepped, Glycerol stocked
| Miniprepped, Glycerol stocked

Revision as of 05:09, 5 July 2008

BioBricks Used/Made

  • Put info here of BioBricks taken from the kit
Brick ID BioBrick No. Description Condtion Location Antibiotic Resistance Size
P3 7F J58102 OmpC-RBS-GFP-Ter Miniprepped, Glycerol stocked -20°C Amp 991bp
P1 9G R0083 OmpC mutated Promoter Miniprepped, Glycerol stocked -20°C Amp 78bp
P3 7G J58103 OmpC(mut.)-RBS-GFP-Ter Miniprepped, Glycerol stocked -20°C Amp 961bp
 ?? I13504 RBS-GFP-Ter  ??  ??  ??  ??
P2 8A I13522 pTetR-RBS-GFP-Ter Miniprepped, Glycerol stocked -20°C Amp 937bp
P0 4G R0062 Promoter (luxR & HSL regulated -- lux pR) Miniprepped, Glycerol stocked -20°C Amp 55bp
P2 8F J23023 Lock 3b Miniprepped -20 Amp 41bp
P0 1A B0011 Terminator Miniprepped -20 Amp  ??
P0 1B B0012 Terminator Miniprepped -20 Amp  ??
P0 1C B0013 Terminator Miniprepped -20 Amp  ??
P2 8H J23032 Lock 3d Miniprepped -20 Amp 43bp
P0 1H B0024 double terminator Miniprepped -20 Amp  ??
P2 8G J23031 lock 3C Miniprepped -20 Amp 42bp
P2 8B J23007 Key 3b Miniprepped, Glycerol stocked -20 Amp 27bp
P2 8C J23008 Key 3c Miniprepped, Sequenced -20 Amp 94bp
P2 8D J23009 Key 3d Miniprepped, Glycerol stocked -20 Amp 97bp
P1 4B E0240 RBS-GFP-Ter Miniprepped, Glycerol stocked -20 Amp 876bp
P0 3E C0061 autoinducer synthetase for AHL Miniprepped, Glycerol stocked -20 Amp 618bp
P3 8C J36846 Lpp-OmpA(46-159)-Streptavidin wild-type + His6 Miniprepped, Glycerol stocked -20 Amp 850bp
P0 3F C0062 luxR repressor/activator, (no LVA?) Miniprepped, Glycerol stocked -20 Amp 756 bp
P0 4C R0040 Miniprepped, Glycerol stocked -20 Amp 54bp
P1 12F I13602 Tet operator with CFP reporter (with LVA tag) [R/Tc+] Miniprepped, Glycerol stocked -20 Amp 979bp
P0 2G C0040 tetracycline repressor from transposon Tn10 (+LVA) Miniprepped, Glycerol stocked -20 Amp 660 bp
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