IGEM:Melbourne/2008/Notebook/BioBricks Used/Made: Difference between revisions
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| Line 14: | Line 14: | ||
|- | |- | ||
| P3 7F | | P3 7F | ||
| J58102 | | [http://partsregistry.org/wiki/index.php?title=Part:BBa_J58102 J58102] | ||
| OmpC-RBS-GFP-Ter | | OmpC-RBS-GFP-Ter | ||
| Miniprepped, Glycerol stocked | | Miniprepped, Glycerol stocked | ||
| Line 22: | Line 22: | ||
|- | |- | ||
| P1 9G | | P1 9G | ||
| R0083 | | [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0083 R0083] | ||
| OmpC mutated Promoter | | OmpC mutated Promoter | ||
| Miniprepped, Glycerol stocked | | Miniprepped, Glycerol stocked | ||
| Line 30: | Line 30: | ||
|- | |- | ||
| P3 7G | | P3 7G | ||
| J58103 | | [http://partsregistry.org/wiki/index.php?title=Part:BBa_J58103 J58103] | ||
| OmpC(mut.)-RBS-GFP-Ter | | OmpC(mut.)-RBS-GFP-Ter | ||
| Miniprepped, Glycerol stocked | | Miniprepped, Glycerol stocked | ||
| Line 46: | Line 46: | ||
|- | |- | ||
| P2 8A | | P2 8A | ||
| I13522 | | [http://partsregistry.org/wiki/index.php?title=Part:BBa_I13522 I13522] | ||
| pTetR-RBS-GFP-Ter | | pTetR-RBS-GFP-Ter | ||
| Miniprepped, Glycerol stocked | | Miniprepped, Glycerol stocked | ||
| Line 54: | Line 54: | ||
|- | |- | ||
| P0 4G | | P0 4G | ||
| R0062 | | [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0062 R0062] | ||
| Promoter (luxR & HSL regulated -- lux pR) | | Promoter (luxR & HSL regulated -- lux pR) | ||
| Miniprepped, Glycerol stocked | | Miniprepped, Glycerol stocked | ||
| Line 62: | Line 62: | ||
|- | |- | ||
| P2 8F | | P2 8F | ||
| J23023 | | [http://partsregistry.org/wiki/index.php?title=Part:BBa_J23023 J23023] | ||
| Lock 3b | | Lock 3b | ||
| Miniprepped | | Miniprepped | ||
| Line 70: | Line 70: | ||
|- | |- | ||
| P0 1A | | P0 1A | ||
| B0011 | | [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0011 B0011] | ||
| Terminator | | Terminator | ||
| Miniprepped | | Miniprepped | ||
| Line 78: | Line 78: | ||
|- | |- | ||
| P0 1B | | P0 1B | ||
| B0012 | | [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0012 B0012] | ||
| Terminator | | Terminator | ||
| Miniprepped | | Miniprepped | ||
| Line 86: | Line 86: | ||
|- | |- | ||
| P0 1C | | P0 1C | ||
| B0013 | | [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0013 B0013] | ||
| Terminator | | Terminator | ||
| Miniprepped | | Miniprepped | ||
| Line 94: | Line 94: | ||
|- | |- | ||
| P2 8H | | P2 8H | ||
| J23032 | | [http://partsregistry.org/wiki/index.php?title=Part:BBa_J23032 J23032] | ||
| Lock 3d | | Lock 3d | ||
| Miniprepped | | Miniprepped | ||
| Line 102: | Line 102: | ||
|- | |- | ||
| P0 1H | | P0 1H | ||
| B0024 | | [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0024 B0024] | ||
| double terminator | | double terminator | ||
| Miniprepped | | Miniprepped | ||
| Line 110: | Line 110: | ||
|- | |- | ||
| P2 8G | | P2 8G | ||
| J23031 | | [http://partsregistry.org/wiki/index.php?title=Part:BBa_J23031 J23031] | ||
| lock 3C | | lock 3C | ||
| Miniprepped | | Miniprepped | ||
| Line 118: | Line 118: | ||
|- | |- | ||
| P2 8B | | P2 8B | ||
| J23007 | | [http://partsregistry.org/wiki/index.php?title=Part:BBa_J23007 J23007] | ||
| Key 3b | | Key 3b | ||
| Miniprepped, Glycerol stocked | | Miniprepped, Glycerol stocked | ||
| Line 126: | Line 126: | ||
|- | |- | ||
| P2 8C | | P2 8C | ||
| J23008 | | [http://partsregistry.org/wiki/index.php?title=Part:BBa_J23008 J23008] | ||
| Key 3c | | Key 3c | ||
| Miniprepped, Sequenced | | Miniprepped, Sequenced | ||
| Line 134: | Line 134: | ||
|- | |- | ||
| P2 8D | | P2 8D | ||
| J23009 | | [http://partsregistry.org/wiki/index.php?title=Part:BBa_J23009 J23009] | ||
| Key 3d | | Key 3d | ||
| Miniprepped, Glycerol stocked | | Miniprepped, Glycerol stocked | ||
| Line 142: | Line 142: | ||
|- | |- | ||
| P1 4B | | P1 4B | ||
| E0240 | | [http://partsregistry.org/wiki/index.php?title=Part:BBa_E0240 E0240] | ||
| RBS-GFP-Ter | | RBS-GFP-Ter | ||
| Miniprepped, Glycerol stocked | | Miniprepped, Glycerol stocked | ||
| Line 150: | Line 150: | ||
|- | |- | ||
| P0 3E | | P0 3E | ||
| C0061 | | [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0061 C0061] | ||
| autoinducer synthetase for AHL | | autoinducer synthetase for AHL | ||
| Miniprepped, Glycerol stocked | | Miniprepped, Glycerol stocked | ||
| Line 156: | Line 156: | ||
| Amp | | Amp | ||
| 618bp | | 618bp | ||
|- | |- | ||
| P3 8C | | P3 8C | ||
| J36846 | | [http://partsregistry.org/wiki/index.php?title=Part:BBa_J36846 J36846] | ||
| Lpp-OmpA(46-159)-Streptavidin wild-type + His6 | | Lpp-OmpA(46-159)-Streptavidin wild-type + His6 | ||
| Miniprepped, Glycerol stocked | | Miniprepped, Glycerol stocked | ||
| Line 174: | Line 166: | ||
|- | |- | ||
| P0 3F | | P0 3F | ||
| C0062 | | [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0062 C0062] | ||
| luxR repressor/activator, (no LVA?) | | luxR repressor/activator, (no LVA?) | ||
| Miniprepped, Glycerol stocked | | Miniprepped, Glycerol stocked | ||
| Line 182: | Line 174: | ||
|- | |- | ||
| P0 4C | | P0 4C | ||
| R0040 | | [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0040 R0040] | ||
| Miniprepped, Glycerol stocked | | Miniprepped, Glycerol stocked | ||
| -20 | | -20 | ||
| Line 190: | Line 181: | ||
|- | |- | ||
| P1 12F | | P1 12F | ||
| I13602 | | [http://partsregistry.org/wiki/index.php?title=Part:BBa_I13602 I13602] | ||
| Tet operator with CFP reporter (with LVA tag) [R/Tc+] | | Tet operator with CFP reporter (with LVA tag) [R/Tc+] | ||
| Miniprepped, Glycerol stocked | | Miniprepped, Glycerol stocked | ||
| Line 198: | Line 189: | ||
|- | |- | ||
| P0 2G | | P0 2G | ||
| C0040 | | [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0040 C0040] | ||
| tetracycline repressor from transposon Tn10 (+LVA) | | tetracycline repressor from transposon Tn10 (+LVA) | ||
| Miniprepped, Glycerol stocked | | Miniprepped, Glycerol stocked | ||
Revision as of 02:09, 5 July 2008
BioBricks Used/Made
- Put info here of BioBricks taken from the kit
| Brick ID | BioBrick No. | Description | Condtion | Location | Antibiotic Resistance | Size |
|---|---|---|---|---|---|---|
| P3 7F | J58102 | OmpC-RBS-GFP-Ter | Miniprepped, Glycerol stocked | -20°C | Amp | 991bp |
| P1 9G | R0083 | OmpC mutated Promoter | Miniprepped, Glycerol stocked | -20°C | Amp | 78bp |
| P3 7G | J58103 | OmpC(mut.)-RBS-GFP-Ter | Miniprepped, Glycerol stocked | -20°C | Amp | 961bp |
| ?? | I13504 | RBS-GFP-Ter | ?? | ?? | ?? | ?? |
| P2 8A | I13522 | pTetR-RBS-GFP-Ter | Miniprepped, Glycerol stocked | -20°C | Amp | 937bp |
| P0 4G | R0062 | Promoter (luxR & HSL regulated -- lux pR) | Miniprepped, Glycerol stocked | -20°C | Amp | 55bp |
| P2 8F | J23023 | Lock 3b | Miniprepped | -20 | Amp | 41bp |
| P0 1A | B0011 | Terminator | Miniprepped | -20 | Amp | ?? |
| P0 1B | B0012 | Terminator | Miniprepped | -20 | Amp | ?? |
| P0 1C | B0013 | Terminator | Miniprepped | -20 | Amp | ?? |
| P2 8H | J23032 | Lock 3d | Miniprepped | -20 | Amp | 43bp |
| P0 1H | B0024 | double terminator | Miniprepped | -20 | Amp | ?? |
| P2 8G | J23031 | lock 3C | Miniprepped | -20 | Amp | 42bp |
| P2 8B | J23007 | Key 3b | Miniprepped, Glycerol stocked | -20 | Amp | 27bp |
| P2 8C | J23008 | Key 3c | Miniprepped, Sequenced | -20 | Amp | 94bp |
| P2 8D | J23009 | Key 3d | Miniprepped, Glycerol stocked | -20 | Amp | 97bp |
| P1 4B | E0240 | RBS-GFP-Ter | Miniprepped, Glycerol stocked | -20 | Amp | 876bp |
| P0 3E | C0061 | autoinducer synthetase for AHL | Miniprepped, Glycerol stocked | -20 | Amp | 618bp |
| P3 8C | J36846 | Lpp-OmpA(46-159)-Streptavidin wild-type + His6 | Miniprepped, Glycerol stocked | -20 | Amp | 850bp |
| P0 3F | C0062 | luxR repressor/activator, (no LVA?) | Miniprepped, Glycerol stocked | -20 | Amp | 756 bp |
| P0 4C | R0040 | Miniprepped, Glycerol stocked | -20 | Amp | 54bp | |
| P1 12F | I13602 | Tet operator with CFP reporter (with LVA tag) [R/Tc+] | Miniprepped, Glycerol stocked | -20 | Amp | 979bp |
| P0 2G | C0040 | tetracycline repressor from transposon Tn10 (+LVA) | Miniprepped, Glycerol stocked | -20 | Amp | 660 bp |
