IGEM:Melbourne/2008/Notebook/BioBricks Used/Made

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| Amp
| Amp
| 660 bp
| 660 bp
 +
|-
 +
| P13 5C
 +
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_I0500 I0500]
 +
| Inducible pBad/araC
 +
| Miniprepped, Glycerol stocked
 +
| -20
 +
| Kan
 +
| 1210bp
 +
|-
 +
| P13 5E
 +
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0073 R0073]
 +
| Promoter (Mnt regulated)
 +
| Miniprepped, Glycerol stocked
 +
| -20
 +
| Kan
 +
| 67 bp
 +
|-
 +
| P0 2E
 +
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 B0034]
 +
| RBS (Elowitz 1999) -- defines RBS efficiency, based on Elowitz repressilator
 +
| Miniprepped, Glycerol stocked
 +
| -20
 +
| Amp
 +
| 12 bp
|-
|-
|}
|}

Revision as of 21:40, 5 July 2008

BioBricks Used/Made

  • Put info here of BioBricks taken from the kit
Brick ID BioBrick No. Description Condtion Location Antibiotic Resistance Size
P3 7F J58102 OmpC-RBS-GFP-Ter Miniprepped, Glycerol stocked -20°C Amp 991bp
P1 9G R0083 OmpC mutated Promoter Miniprepped, Glycerol stocked -20°C Amp 78bp
P3 7G J58103 OmpC(mut.)-RBS-GFP-Ter Miniprepped, Glycerol stocked -20°C Amp 961bp
 ?? I13504 RBS-GFP-Ter  ??  ??  ??  ??
P2 8A I13522 pTetR-RBS-GFP-Ter Miniprepped, Glycerol stocked -20°C Amp 937bp
P0 4G R0062 Promoter (luxR & HSL regulated -- lux pR) Miniprepped, Glycerol stocked -20°C Amp 55bp
P2 8F J23023 Lock 3b Miniprepped -20 Amp 41bp
P0 1A B0011 Terminator Miniprepped -20 Amp  ??
P0 1B B0012 Terminator Miniprepped -20 Amp  ??
P0 1C B0013 Terminator Miniprepped -20 Amp  ??
P2 8H J23032 Lock 3d Miniprepped -20 Amp 43bp
P0 1H B0024 double terminator Miniprepped -20 Amp  ??
P2 8G J23031 lock 3C Miniprepped -20 Amp 42bp
P2 8B J23007 Key 3b Miniprepped, Glycerol stocked -20 Amp 27bp
P2 8C J23008 Key 3c Miniprepped, Sequenced -20 Amp 94bp
P2 8D J23009 Key 3d Miniprepped, Glycerol stocked -20 Amp 97bp
P1 4B E0240 RBS-GFP-Ter Miniprepped, Glycerol stocked -20 Amp 876bp
P0 3E C0061 autoinducer synthetase for AHL Miniprepped, Glycerol stocked -20 Amp 618bp
P3 8C J36846 Lpp-OmpA(46-159)-Streptavidin wild-type + His6 Miniprepped, Glycerol stocked -20 Amp 850bp
P0 3F C0062 luxR repressor/activator, (no LVA?) Miniprepped, Glycerol stocked -20 Amp 756 bp
P0 4C R0040 TetR repressible promoter Miniprepped, Glycerol stocked -20 Amp 54bp
P1 12F I13602 Tet operator with CFP reporter (with LVA tag) [R/Tc+] Miniprepped, Glycerol stocked -20 Amp 979bp
P0 2G C0040 tetracycline repressor from transposon Tn10 (+LVA) Miniprepped, Glycerol stocked -20 Amp 660 bp
P13 5C I0500 Inducible pBad/araC Miniprepped, Glycerol stocked -20 Kan 1210bp
P13 5E R0073 Promoter (Mnt regulated) Miniprepped, Glycerol stocked -20 Kan 67 bp
P0 2E B0034 RBS (Elowitz 1999) -- defines RBS efficiency, based on Elowitz repressilator Miniprepped, Glycerol stocked -20 Amp 12 bp
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