IGEM:Melbourne/2008/Notebook/BioBricks Used/Made

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Line 12: Line 12:
! Antibiotic Resistance
! Antibiotic Resistance
! Size
! Size
 +
! Sequencing Result
|-
|-
| P3 7F
| P3 7F
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_J58102 J58102]
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_J58102 J58102]
| OmpC-RBS-GFP-Ter
| OmpC-RBS-GFP-Ter
-
| Miniprepped, Glycerol stocked
+
| Miniprepped, Glycerol stocked, Sequenced
| -20°C  
| -20°C  
| Amp
| Amp
Line 24: Line 25:
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0083 R0083]
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0083 R0083]
| OmpC mutated Promoter
| OmpC mutated Promoter
-
| Miniprepped, Glycerol stocked
+
| Miniprepped, Glycerol stocked, Sequenced
| -20°C  
| -20°C  
| Amp
| Amp
Line 32: Line 33:
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_J58103 J58103]
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_J58103 J58103]
| OmpC(mut.)-RBS-GFP-Ter
| OmpC(mut.)-RBS-GFP-Ter
-
| Miniprepped, Glycerol stocked
+
| Miniprepped, Glycerol stocked, Sequenced
| -20°C  
| -20°C  
| Amp
| Amp
Line 48: Line 49:
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_I13522 I13522]
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_I13522 I13522]
| pTetR-RBS-GFP-Ter
| pTetR-RBS-GFP-Ter
-
| Miniprepped, Glycerol stocked
+
| Miniprepped, Glycerol stocked, Sequenced
| -20°C  
| -20°C  
| Amp
| Amp
| 937bp
| 937bp
 +
| Good
|-
|-
| P0 4G
| P0 4G
Line 64: Line 66:
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_J23030 J23030]
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_J23030 J23030]
| Lock 3b
| Lock 3b
-
| Miniprepped
+
| Miniprepped, Sequenced
| -20  
| -20  
| Amp
| Amp
| 41bp
| 41bp
 +
| Good
|-
|-
| P0 1A
| P0 1A
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0011 B0011]
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0011 B0011]
| Terminator
| Terminator
-
| Miniprepped
+
| Miniprepped, Sequenced
| -20
| -20
| Amp
| Amp
Line 80: Line 83:
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0012 B0012]
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0012 B0012]
| Terminator
| Terminator
-
| Miniprepped
+
| Miniprepped, Sequenced
| -20
| -20
| Amp
| Amp
Line 88: Line 91:
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0013 B0013]
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0013 B0013]
| Terminator
| Terminator
-
| Miniprepped
+
| Miniprepped, Sequenced
| -20
| -20
| Amp
| Amp
Line 96: Line 99:
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_J23032 J23032]
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_J23032 J23032]
| Lock 3d
| Lock 3d
-
| Miniprepped
+
| Miniprepped, Sequenced
| -20
| -20
| Amp
| Amp
| 43bp
| 43bp
 +
| Good
|-
|-
| P0 1H
| P0 1H
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0024 B0024]
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0024 B0024]
| double terminator
| double terminator
-
| Miniprepped
+
| Miniprepped, Sequenced
| -20
| -20
| Amp
| Amp
| 95
| 95
 +
| Good
|-
|-
| P2 8G
| P2 8G
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_J23031 J23031]
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_J23031 J23031]
| lock 3C
| lock 3C
-
| Miniprepped
+
| Miniprepped, Sequenced
| -20
| -20
| Amp
| Amp
| 42bp
| 42bp
 +
| Bad
|-
|-
| P2 8B
| P2 8B
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_J23007 J23007]
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_J23007 J23007]
| Key 3b
| Key 3b
-
| Miniprepped, Glycerol stocked
+
| Miniprepped, Glycerol stocked, Sequenced
| -20
| -20
| Amp
| Amp
| 27bp
| 27bp
 +
| Bad
|-
|-
| P2 8C
| P2 8C
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_J23008 J23008]
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_J23008 J23008]
| Key 3c
| Key 3c
-
| Miniprepped, Sequenced
+
| Miniprepped, Sequenced, Sequenced
| -20
| -20
| Amp
| Amp
| 94bp
| 94bp
 +
| Good
|-
|-
| P2 8D
| P2 8D
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_J23009 J23009]
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_J23009 J23009]
| Key 3d
| Key 3d
-
| Miniprepped, Glycerol stocked
+
| Miniprepped, Glycerol stocked, Sequenced
| -20
| -20
| Amp
| Amp
| 97bp
| 97bp
 +
| Bad
|-
|-
| P1 4B
| P1 4B
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_E0240 E0240]
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_E0240 E0240]
| RBS-GFP-Ter
| RBS-GFP-Ter
-
| Miniprepped, Glycerol stocked
+
| Miniprepped, Glycerol stocked, Sequenced
| -20
| -20
| Amp
| Amp
Line 192: Line 201:
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0040 C0040]
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0040 C0040]
| tetracycline repressor from transposon Tn10 (+LVA)
| tetracycline repressor from transposon Tn10 (+LVA)
-
| Miniprepped, Glycerol stocked
+
| Miniprepped, Glycerol stocked, Sequenced
| -20
| -20
| Amp
| Amp
Line 216: Line 225:
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 B0034]
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 B0034]
| RBS (Elowitz 1999) -- defines RBS efficiency, based on Elowitz repressilator
| RBS (Elowitz 1999) -- defines RBS efficiency, based on Elowitz repressilator
-
| Miniprepped, Glycerol stocked
+
| Miniprepped, Glycerol stocked, Sequenced
| -20
| -20
| Amp
| Amp
Line 224: Line 233:
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 B0015]
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 B0015]
| double terminator (B0010-B0012)
| double terminator (B0010-B0012)
-
| Miniprepped, Glycerol stocked
+
| Miniprepped, Glycerol stocked, Sequenced
| -20
| -20
| Amp, Kan
| Amp, Kan
Line 232: Line 241:
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0010 R0010]
| [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0010 R0010]
| promoter (lacI regulated)
| promoter (lacI regulated)
-
| Miniprepped, Glycerol stocked
+
| Miniprepped, Glycerol stocked, Sequenced
| -20
| -20
| Amp
| Amp
Line 240: Line 249:
| [http://partsregistry.org/Part:BBa_E0040 E0040]
| [http://partsregistry.org/Part:BBa_E0040 E0040]
| GFP (no promoter, no RBS, no Ter)
| GFP (no promoter, no RBS, no Ter)
-
| Miniprepped, Glycerol stocked
+
| Miniprepped, Glycerol stocked, Sequenced
| -20
| -20
| Amp
| Amp
| 720 bp
| 720 bp
 +
| Bad
|-
|-
|}
|}

Revision as of 04:02, 11 July 2008

BioBricks Used/Made

  • Put info here of BioBricks taken from the kit
Brick ID BioBrick No. Description Condtion Location Antibiotic Resistance Size Sequencing Result
P3 7F J58102 OmpC-RBS-GFP-Ter Miniprepped, Glycerol stocked, Sequenced -20°C Amp 991bp
P1 9G R0083 OmpC mutated Promoter Miniprepped, Glycerol stocked, Sequenced -20°C Amp 78bp
P3 7G J58103 OmpC(mut.)-RBS-GFP-Ter Miniprepped, Glycerol stocked, Sequenced -20°C Amp 961bp
 ?? I13504 RBS-GFP-Ter  ??  ??  ??  ??
P2 8A I13522 pTetR-RBS-GFP-Ter Miniprepped, Glycerol stocked, Sequenced -20°C Amp 937bp Good
P0 4G R0062 Promoter (luxR & HSL regulated -- lux pR) Miniprepped, Glycerol stocked -20°C Amp 55bp
P2 8F J23030 Lock 3b Miniprepped, Sequenced -20 Amp 41bp Good
P0 1A B0011 Terminator Miniprepped, Sequenced -20 Amp 46
P0 1B B0012 Terminator Miniprepped, Sequenced -20 Amp 41
P0 1C B0013 Terminator Miniprepped, Sequenced -20 Amp 47
P2 8H J23032 Lock 3d Miniprepped, Sequenced -20 Amp 43bp Good
P0 1H B0024 double terminator Miniprepped, Sequenced -20 Amp 95 Good
P2 8G J23031 lock 3C Miniprepped, Sequenced -20 Amp 42bp Bad
P2 8B J23007 Key 3b Miniprepped, Glycerol stocked, Sequenced -20 Amp 27bp Bad
P2 8C J23008 Key 3c Miniprepped, Sequenced, Sequenced -20 Amp 94bp Good
P2 8D J23009 Key 3d Miniprepped, Glycerol stocked, Sequenced -20 Amp 97bp Bad
P1 4B E0240 RBS-GFP-Ter Miniprepped, Glycerol stocked, Sequenced -20 Amp 876bp
P0 3E C0061 autoinducer synthetase for AHL Miniprepped, Glycerol stocked -20 Amp 618bp
P3 8C J36846 Lpp-OmpA(46-159)-Streptavidin wild-type + His6 Miniprepped, Glycerol stocked -20 Amp 850bp
P0 3F C0062 luxR repressor/activator, (no LVA?) Miniprepped, Glycerol stocked -20 Amp 756 bp
P0 4C R0040 TetR repressible promoter Miniprepped, Glycerol stocked -20 Amp 54bp
P1 12F I13602 Tet operator with CFP reporter (with LVA tag) [R/Tc+] Miniprepped, Glycerol stocked -20 Amp 979bp
P0 2G C0040 tetracycline repressor from transposon Tn10 (+LVA) Miniprepped, Glycerol stocked, Sequenced -20 Amp 660 bp
P13 5C I0500 Inducible pBad/araC Miniprepped, Glycerol stocked -20 Kan 1210bp
P13 5E R0073 Promoter (Mnt regulated) Miniprepped, Glycerol stocked -20 Kan 67 bp
P0 2E B0034 RBS (Elowitz 1999) -- defines RBS efficiency, based on Elowitz repressilator Miniprepped, Glycerol stocked, Sequenced -20 Amp 12 bp
P16 5F B0015 double terminator (B0010-B0012) Miniprepped, Glycerol stocked, Sequenced -20 Amp, Kan 129 bp
P2 5E R0010 promoter (lacI regulated) Miniprepped, Glycerol stocked, Sequenced -20 Amp 200 bp
P1 7E E0040 GFP (no promoter, no RBS, no Ter) Miniprepped, Glycerol stocked, Sequenced -20 Amp 720 bp Bad
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