IGEM:Melbourne/2008/Notebook/BioBricks Used/Made

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| P1 7E

Revision as of 04:16, 11 July 2008

BioBricks Used/Made

  • Put info here of BioBricks taken from the kit
Brick ID BioBrick No. Description Condtion Location Antibiotic Resistance Size Sequencing Result
P3 7F J58102 OmpC-RBS-GFP-Ter Miniprepped, Glycerol stocked, Sequenced -20°C Amp 991bp
P1 9G R0083 OmpC mutated Promoter Miniprepped, Glycerol stocked, Sequenced -20°C Amp 78bp
P3 7G J58103 OmpC(mut.)-RBS-GFP-Ter Miniprepped, Glycerol stocked, Sequenced -20°C Amp 961bp
 ?? I13504 RBS-GFP-Ter  ??  ??  ??  ??
P2 8A I13522 pTetR-RBS-GFP-Ter Miniprepped, Glycerol stocked, Sequenced -20°C Amp 937bp Good
P0 4G R0062 Promoter (luxR & HSL regulated -- lux pR) Miniprepped, Glycerol stocked -20°C Amp 55bp
P2 8F J23030 Lock 3b Miniprepped, Sequenced -20 Amp 41bp Good
P0 1A B0011 Terminator Miniprepped, Sequenced -20 Amp 46
P0 1B B0012 Terminator Miniprepped, Sequenced -20 Amp 41
P0 1C B0013 Terminator Miniprepped, Sequenced -20 Amp 47
P2 8H J23032 Lock 3d Miniprepped, Sequenced -20 Amp 43bp Good
P0 1H B0024 double terminator Miniprepped, Sequenced -20 Amp 95 Good
P2 8G J23031 lock 3C Miniprepped, Sequenced -20 Amp 42bp Bad
P2 8B J23007 Key 3b Miniprepped, Glycerol stocked, Sequenced -20 Amp 27bp Bad
P2 8C J23008 Key 3c Miniprepped, Sequenced, Sequenced -20 Amp 94bp Good
P2 8D J23009 Key 3d Miniprepped, Glycerol stocked, Sequenced -20 Amp 97bp Bad
P1 4B E0240 RBS-GFP-Ter Miniprepped, Glycerol stocked, Sequenced -20 Amp 876bp
P0 3E C0061 autoinducer synthetase for AHL Miniprepped, Glycerol stocked -20 Amp 618bp
P3 8C J36846 Lpp-OmpA(46-159)-Streptavidin wild-type + His6 Miniprepped, Glycerol stocked -20 Amp 850bp
P0 3F C0062 luxR repressor/activator, (no LVA?) Miniprepped, Glycerol stocked -20 Amp 756 bp
P0 4C R0040 TetR repressible promoter Miniprepped, Glycerol stocked -20 Amp 54bp
P1 12F I13602 Tet operator with CFP reporter (with LVA tag) [R/Tc+] Miniprepped, Glycerol stocked -20 Amp 979bp
P0 2G C0040 tetracycline repressor from transposon Tn10 (+LVA) Miniprepped, Glycerol stocked, Sequenced -20 Amp 660 bp
P13 5C I0500 Inducible pBad/araC Miniprepped, Glycerol stocked -20 Kan 1210bp
P13 5E R0073 Promoter (Mnt regulated) Miniprepped, Glycerol stocked -20 Kan 67 bp
P0 2E B0034 RBS (Elowitz 1999) -- defines RBS efficiency, based on Elowitz repressilator Miniprepped, Glycerol stocked, Sequenced -20 Amp 12 bp
P16 5F B0015 double terminator (B0010-B0012) Miniprepped, Glycerol stocked, Sequenced -20 Amp, Kan 129 bp
P2 5E R0010 promoter (lacI regulated) Miniprepped, Glycerol stocked, Sequenced -20 Amp 200 bp Bad
P1 7E E0040 GFP (no promoter, no RBS, no Ter) Miniprepped, Glycerol stocked, Sequenced -20 Amp 720 bp Bad
P6 3D I763033 pTEtR-RBS-mRFP-Ter-Ter Miniprepped -20 Amp 923 bp
P2 9A J23033 Lock 3e Miniprepped -20 Amp 40 bp
P13 9H I15009 phycocyanobilin, convert BV to PCB Miniprepped -20 Kan 750 bp
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