IGEM:Melbourne/2008/Notebook/BioClock/2008/05/20: Difference between revisions

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-Stephen / Simon
-Stephen / Simon
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* Suspension and transformation of P1002 8B-8D, 8F-8H. These are three pairs of riobkeys and locks.
* Suspension and transformation of P1002 8B-8D, 8F-8H. These are three pairs of riobkeys and locks.
* Re-transformation of P1000 1A-C, 1G-1H, using the plasmids Haozhen suspended, added another 5ul of TE to each today.
* Re-transformation of P1000 1A-C, 1G-1H, using the plasmids Haozhen suspended, added another 5ul of TE to each today.
- jason
- jason
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Revision as of 03:49, 20 May 2008

Melbourne iGEM 2008 -- BioClock <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Done today:

  • Preparation of pL-pCB and pCph8 for sequencing
    • 100μM primer stock stored in red light box in -20°C freezer
  • O/N culture of pL-pCB and pCph8 for miniprep tomorrow

-Stephen / Simon

  • Suspension and transformation of P1002 8B-8D, 8F-8H. These are three pairs of riobkeys and locks.
  • Re-transformation of P1000 1A-C, 1G-1H, using the plasmids Haozhen suspended, added another 5ul of TE to each today.

- jason