IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2013/07/30: Difference between revisions

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<b><span style="font-size:16px;">Title:</span></b> Preparation of P. aeruginosa  
<b><span style="font-size:16px;">Title:</span></b> Preparation of P. aeruginosa  


<b>Start Time</b>: 3:00 PM
<b>Start Time:</b> 3:00 PM


<b>Purpose</b>: To make a stock of P. aeruginosa for future lab work.
<b>Purpose:</b> To make a stock of P. aeruginosa for future lab work.


<b>Protocol</b>: Inoculated LB broth with P. aeruginosa from plated bacteria, no antibiotic. Inoculated another tube of LB broth with liquid P. aeruginosa culture, 10ml into 10ml.
<b>Protocol:</b> Inoculated LB broth with P. aeruginosa from plated bacteria, no antibiotic. Inoculated another tube of LB broth with liquid P. aeruginosa culture, 10ml into 10ml.
   
   
Left both cultures in 37C incubator overnight.
Left both cultures in 37C incubator overnight.
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Placed cell solutions in 1.5ml Epi tubes and placed in -20C freezer.
Placed cell solutions in 1.5ml Epi tubes and placed in -20C freezer.


<b>Products</b>:
<b>Products:</b>  
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<b>Stop Time</b>: 5:00 PM  
<b>Stop Time:</b> 5:00 PM  


<b>Next</b>: Amplify DNA segments from prepared P. aeruginosa samples.
<b>Next:</b> Amplify DNA segments from prepared P. aeruginosa samples.




Revision as of 15:58, 15 December 2013

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Date: 7/30/13 People in lab: Emily Puleo, Kelsey Crossen

Title: Preparation of P. aeruginosa

Start Time: 3:00 PM

Purpose: To make a stock of P. aeruginosa for future lab work.

Protocol: Inoculated LB broth with P. aeruginosa from plated bacteria, no antibiotic. Inoculated another tube of LB broth with liquid P. aeruginosa culture, 10ml into 10ml.

Left both cultures in 37C incubator overnight.

Spun down 1ml cultures of P. aeruginosa, discarded supernatant, and re-suspended in 1ml Milli-Q water.

Placed cell solutions in 1.5ml Epi tubes and placed in -20C freezer.

Products:

Sample Label Description Source Label Quantity
P. aeruginosa 7/30 P. aeruginosa culture P. aeruginosa 2
P. aeruginosa 7/30 P. aeruginosa culture, frozen P. aeruginosa 10


Stop Time: 5:00 PM

Next: Amplify DNA segments from prepared P. aeruginosa samples.



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