IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2013/07/31: Difference between revisions
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|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:20px;"> Cyanobacteria Mediated Filtration of Coal Flue Gas</span> | |style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:20px;"> Cyanobacteria Mediated Filtration of Coal Flue Gas</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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<b>Notes:</b> Different amounts of template used to find optimum amount needed. | <b>Notes:</b> Different amounts of template used to find optimum amount needed. | ||
Forward primer: (24.2*10^-9)/(0.33*10^-3) mol/L = 73 uM, (73uM)(10uL)=(0.73uM)V2 V2 = 1000uL | Forward primer: (24.2*10^-9)/(0.33*10^-3) mol/L = 73 uM, (73uM)(10uL)=(0.73uM)V2, V2 = 1000uL | ||
Reverse primer: (28.7*10^-9)/(0.33*10^-3) mol/L = 87 uM, (87uM)(10uL)=(0.87uM)V2 V2 = 1000uL | Reverse primer: (28.7*10^-9)/(0.33*10^-3) mol/L = 87 uM, (87uM)(10uL)=(0.87uM)V2, V2 = 1000uL | ||
Used Dr. Shannon's thermocycler, copied "igem_pcr" program | Used Dr. Shannon's thermocycler, copied "igem_pcr" program |
Latest revision as of 23:37, 26 September 2017
Cyanobacteria Mediated Filtration of Coal Flue Gas | Main project page Previous entry Next entry | ||||||||||||||||||||||||
Date: 7/31/13 People in lab: Emily Puleo, Kelsey CrossenTitle: PCR of norCB, dilution of norCB primers Start Time: 4:57 PM Purpose: PCR norCB from P. aeruginosa, adding iGEM prefix and suffix; to prepare primers for future lab work Protocol: LTM ed. 2 pg. 48 Exceptions: 1. Less than 0.1 uM final primer concentration. Products:
Results: Sample 3 had too little product and was discarded. Notes: Different amounts of template used to find optimum amount needed. Forward primer: (24.2*10^-9)/(0.33*10^-3) mol/L = 73 uM, (73uM)(10uL)=(0.73uM)V2, V2 = 1000uL Reverse primer: (28.7*10^-9)/(0.33*10^-3) mol/L = 87 uM, (87uM)(10uL)=(0.87uM)V2, V2 = 1000uL Used Dr. Shannon's thermocycler, copied "igem_pcr" program Stop Time: 9:00 PM Next: Run samples on a gel, extract the stronger band from between them, sequence the result.
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