IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2013/08/02: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Levi Palmer (talk | contribs) (Autocreate 2013/08/02 Entry for IGEM:Missouri_University_of_Science_and_Technology/2009/Notebook/Cyanobacteria_Mediated_Filtration_of_Coal_Flue) |
(fix raw html notebook nav) |
||
(One intermediate revision by one other user not shown) | |||
Line 2: | Line 2: | ||
|- | |- | ||
|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:20px;"> Cyanobacteria Mediated Filtration of Coal Flue Gas</span> | |style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:20px;"> Cyanobacteria Mediated Filtration of Coal Flue Gas</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
|- | |- | ||
| colspan="2"| | | colspan="2"| | ||
== <b>Date: | == <b>Date: 08/02/13</b> <b>People in lab: Emily Puleo, Kelsey Crossen</b> == | ||
<b><span style="font-size:16px;">Title:</span></b> | <b><span style="font-size:16px;">Title:</span></b> PCR of norCB | ||
<b>Start Time:</b> | <b>Start Time:</b> 4:30 PM | ||
<b>Purpose:</b> | <b>Purpose:</b> To PCR norCB and add iGEM prefix&suffix | ||
<b>Protocol:</b> <b>Exceptions:</b> | <b>Protocol:</b> LTM ed. 2 pg 45-47 <b>Exceptions:</b> 1. Final primer concentration <0.1 uM 2. Decreased annealing temperature to 63C 3. Increased elongation time to 5 minutes | ||
<b>Products:</b> | <b>Products:</b> | ||
Line 21: | Line 21: | ||
! Sample Label !! Description !! Source Label !! Quantity | ! Sample Label !! Description !! Source Label !! Quantity | ||
|- | |- | ||
| | | 8/2/13 A norCB 1 || norCB gene from P. aeruginosa with iGEM prefix and suffix using 7.5 uL template P. aeruginosa || P. aeruginosa 7/30 || 1 | ||
|- | |- | ||
| | | 8/2/13 A norCB 2 || norCB gene from P. aeruginosa with iGEM prefix and suffix using 7.5 uL template P. aeruginosa 2x concentration || P. aerguginosa 7/30 2x in H2O || 1 | ||
|} | |} | ||
<b>Results:</b> | <b>Results:</b> N/A | ||
<b>Notes:</b> | <b>Notes:</b> | ||
<b>Stop Time:</b> | <b>Stop Time:</b> 8:35 PM | ||
<b>Next:</b> | <b>Next:</b> Run samples on a gel. Extract, purify, sequence the better resultant gene product. | ||
Latest revision as of 23:40, 26 September 2017
Cyanobacteria Mediated Filtration of Coal Flue Gas | Main project page Previous entry Next entry | ||||||||||||
Date: 08/02/13 People in lab: Emily Puleo, Kelsey CrossenTitle: PCR of norCB Start Time: 4:30 PM Purpose: To PCR norCB and add iGEM prefix&suffix Protocol: LTM ed. 2 pg 45-47 Exceptions: 1. Final primer concentration <0.1 uM 2. Decreased annealing temperature to 63C 3. Increased elongation time to 5 minutes Products:
Results: N/A Notes: Stop Time: 8:35 PM Next: Run samples on a gel. Extract, purify, sequence the better resultant gene product.
|