IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2013/08/03: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
(Autocreate 2013/08/03 Entry for IGEM:Missouri_University_of_Science_and_Technology/2009/Notebook/Cyanobacteria_Mediated_Filtration_of_Coal_Flue)
 
(fix raw html notebook nav)
 
(6 intermediate revisions by one other user not shown)
Line 1: Line 1:
{|{{table}} width="800"
{|{{table}} width="850"
|-
|-
|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:20px;"> Cyanobacteria Mediated Filtration of Coal Flue Gas</span>
|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:20px;"> Cyanobacteria Mediated Filtration of Coal Flue Gas</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
|-
|-
| colspan="2"|
| colspan="2"|


== <b>Date: MM/DD/YY</b> <b>People in lab: Yourname</b>  ==
== <b>Date: 08/3/13</b> <b>People in lab: Emily Puleo, Kelsey Crossen</b>  (1/2) ==


<b><span style="font-size:16px;">Title:</span></b> Fill in the fields on this page to create a new notebook entry.
<b><span style="font-size:16px;">Title:</span></b> Gel electrophoresis of 8/2/13 A norCB 1 and 2


<b>Start Time:</b>
<b>Start Time:</b> 2:05 PM


<b>Purpose:</b>  
<b>Purpose:</b> Confirm norCB gene with iGEM prefix & suffix. Use the better sample to purify and sequence.


<b>Protocol:</b> <b>Exceptions:</b>
<b>Protocol:</b> LTM ed. 2 pg 45-47 


<b>Products:</b>  
{| border="1" cellpadding="5"
|-
! Well !! 1 !! 2 !! 3 !! 4 !! 5 !! 6 !! 7 !! 8
|-
| Sample || Ladder || 8/2/13 A norCB  1 || 8/2/13 A norCB 2 || || || || ||
|}
 
<b>Products:</b> N/A
 
<b>Results:</b> PICTURE
 
<b>Notes:</b>
 
<b>Stop Time:</b> 3:30 PM
 
<b>Next:</b> The ~1900 bp fragment from 8/2/13 A norCB 2 should be extracted and purified.
 
 
== <b>Date: 08/3/13</b> <b>People in lab: Emily Puleo, Kelsey Crossen</b> (2/2) ==
 
<b><span style="font-size:16px;">Title:</span></b> Gel extraction of ~2000bp fragment from today's gel
 
<b>Start Time:</b> 3:50 PM
 
<b>Purpose:</b> To extract the PCR product of the norCB gene + iGEM prefix & suffix
 
<b>Protocol:</b> IBI Gel extraction <b>Exceptions:</b> 1. Milli-Q used instead of Elution Buffer
 
<b>Products:</b>
{| border="1" cellpadding="5"
{| border="1" cellpadding="5"
|-
|-
! Sample Label !! Description !! Source Label !! Quantity
! Sample Label !! Description !! Source Label !! Quantity
|-
|-
| Entry || Entry || Entry || #
| 8/3/13 GE norCB || norCB w/ iGEM prefix/suffix (~1900 bp) from well 3 || Well 3 8/3/13 Gel ||1
|-
|-
| Entry || Entry || Entry || #
|}
|}


<b>Results:</b>  
<b>Results:</b> N/A


<b>Notes:</b>  
<b>Notes:</b>  


<b>Stop Time:</b>  
<b>Stop Time:</b> 4:45 PM
 
<b>Next:</b>
 


<b>Next:</b> 8/3/13 GE norCB should be ligated into the iGEM vector.


<!-- ## Do not edit below this line unless you know what you are doing. ## -->
<!-- ## Do not edit below this line unless you know what you are doing. ## -->

Latest revision as of 23:40, 26 September 2017

Cyanobacteria Mediated Filtration of Coal Flue Gas Main project page
Previous entry      Next entry

Date: 08/3/13 People in lab: Emily Puleo, Kelsey Crossen (1/2)

Title: Gel electrophoresis of 8/2/13 A norCB 1 and 2

Start Time: 2:05 PM

Purpose: Confirm norCB gene with iGEM prefix & suffix. Use the better sample to purify and sequence.

Protocol: LTM ed. 2 pg 45-47


Well 1 2 3 4 5 6 7 8
Sample Ladder 8/2/13 A norCB 1 8/2/13 A norCB 2

Products: N/A

Results: PICTURE

Notes:

Stop Time: 3:30 PM

Next: The ~1900 bp fragment from 8/2/13 A norCB 2 should be extracted and purified.


Date: 08/3/13 People in lab: Emily Puleo, Kelsey Crossen (2/2)

Title: Gel extraction of ~2000bp fragment from today's gel

Start Time: 3:50 PM

Purpose: To extract the PCR product of the norCB gene + iGEM prefix & suffix

Protocol: IBI Gel extraction Exceptions: 1. Milli-Q used instead of Elution Buffer

Products:

Sample Label Description Source Label Quantity
8/3/13 GE norCB norCB w/ iGEM prefix/suffix (~1900 bp) from well 3 Well 3 8/3/13 Gel 1

Results: N/A

Notes:

Stop Time: 4:45 PM

Next: 8/3/13 GE norCB should be ligated into the iGEM vector.


Retrieved from "https://openwetware.org/mediawiki/index.php?title=IGEM:Missouri_University_of_Science_and_Technology/2009/Notebook/Cyanobacteria_Mediated_Filtration_of_Coal_Flue/2013/08/03&oldid=1013922"