IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2013/08/06: Difference between revisions
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Levi Palmer (talk | contribs) (Autocreate 2013/08/06 Entry for IGEM:Missouri_University_of_Science_and_Technology/2009/Notebook/Cyanobacteria_Mediated_Filtration_of_Coal_Flue) |
Levi Palmer (talk | contribs) |
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== <b>Date: | == <b>Date: 08/06/13</b> <b> Blythe Ferriere, Annie Goo</b> == | ||
<b><span style="font-size:16px;">Title:</span></b> | <b><span style="font-size:16px;">Title:</span></b> People in lab: Dilute primers plus PCR | ||
<b>Start Time:</b> | <b>Start Time:</b> 2:15 PM | ||
<b>Purpose:</b> | <b>Purpose:</b> Dilute primers for PCR reaction of norV gene, PCR norV gene from E. coli K-12 add iGEM prefix-suffix | ||
<b>Protocol:</b> <b>Exceptions:</b> | <b>Protocol:</b> Fill in later pg 10 lab notebook <b>Exceptions:</b> | ||
<b>Products:</b> | <b>Products:</b> | ||
Line 21: | Line 21: | ||
! Sample Label !! Description !! Source Label !! Quantity | ! Sample Label !! Description !! Source Label !! Quantity | ||
|- | |- | ||
| | | 8/6/13 norV A 1 || Amplified norV gene w/ iGEM prefix & suffix using 7.5 uL of template DNA || template DNA 7/31/13 || 3 | ||
|- | |- | ||
| | | 8/6/13 norV A 4 || Amplified norV gene w/ iGEM prefix & suffix using 5 uL of template DNA || Template DNA 7/31/13 || 3 | ||
|} | |} | ||
<b>Results:</b> | <b>Results:</b> N/A | ||
<b>Notes:</b> | <b>Notes:</b> Changed programs about 15 minutes in | ||
<b>Stop Time:</b> | <b>Stop Time:</b> 5:00 PM | ||
<b>Next:</b> | <b>Next:</b> Ligtate into iGEM backbone | ||
Revision as of 17:26, 28 January 2014
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Date: 08/06/13 Blythe Ferriere, Annie GooTitle: People in lab: Dilute primers plus PCR Start Time: 2:15 PM Purpose: Dilute primers for PCR reaction of norV gene, PCR norV gene from E. coli K-12 add iGEM prefix-suffix Protocol: Fill in later pg 10 lab notebook Exceptions: Products:
Results: N/A Notes: Changed programs about 15 minutes in Stop Time: 5:00 PM Next: Ligtate into iGEM backbone
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