IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2013/08/06: Difference between revisions
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<b>Purpose:</b> Dilute primers for PCR reaction of norV gene, PCR norV gene from E. coli K-12 add iGEM prefix-suffix | <b>Purpose:</b> Dilute primers for PCR reaction of norV gene, PCR norV gene from E. coli K-12 add iGEM prefix-suffix | ||
<b>Protocol:</b> | <b>Protocol:</b> Dilution: Forward Primer: 0.39mg, To make 1 ug/uL: add 390 uL milliQ, To make 10ng/uL: add 1 uL of 1 ug/uL solution to 100 uL milliQ, Final Solution: ~0.7 uM | ||
Reverse Primer: 0.38mg, To make 1ug/uL: add 380 uL milliQ, To make 10ng/uL: add 1 uL of 1 ug/uL solution to 100 uL milliQ, Final Solution: ~0.7 uM | |||
<b>Products:</b> | <b>Products:</b> |
Revision as of 10:35, 30 January 2014
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Date: 08/06/13 Blythe Ferriere, Annie GooTitle: People in lab: Dilute primers plus PCR Start Time: 2:15 PM Purpose: Dilute primers for PCR reaction of norV gene, PCR norV gene from E. coli K-12 add iGEM prefix-suffix Protocol: Dilution: Forward Primer: 0.39mg, To make 1 ug/uL: add 390 uL milliQ, To make 10ng/uL: add 1 uL of 1 ug/uL solution to 100 uL milliQ, Final Solution: ~0.7 uM Reverse Primer: 0.38mg, To make 1ug/uL: add 380 uL milliQ, To make 10ng/uL: add 1 uL of 1 ug/uL solution to 100 uL milliQ, Final Solution: ~0.7 uM Products:
Results: N/A Notes: Changed programs about 15 minutes in Stop Time: 5:00 PM Next: Ligtate into iGEM backbone
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