IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2013/08/07: Difference between revisions

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|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:20px;"> Cyanobacteria Mediated Filtration of Coal Flue Gas</span>
|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:20px;"> Cyanobacteria Mediated Filtration of Coal Flue Gas</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
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== <b>Date: MM/DD/YY</b> <b>People in lab: Yourname</b>  ==
== <b>Date: 08/07/13</b> <b>People in lab: Blythe Ferriere</b>  ==


<b><span style="font-size:16px;">Title:</span></b> Fill in the fields on this page to create a new notebook entry.
<b><span style="font-size:16px;">Title:</span></b> Gel Electrophoresis of 8/6/13 A1-A6 PCR reactions


<b>Start Time:</b>
<b>Start Time:</b> 8:45 AM


<b>Purpose:</b>  
<b>Purpose:</b> To check for norV gene product with prefix&suffix from PCR amplification.


<b>Protocol:</b> <b>Exceptions:</b>
<b>Protocol:</b> LTM ed. 2 pg 45-47


<b>Products:</b>  
{| border="1" cellpadding="5"
|-
! Well !! 1 !! 2 !! 3 !! 4 !! 5 !! 6 !! 7 !! 8
|-
| Sample || Ladder || 8/6/13 A1 || 8/6/13 A2 || 8/6/13 A3 || 8/6/13 A4 || 8/6/13 A5 || 8/6/13 A6 || Ladder
|}
 
<b>Products:</b> N/A
 
<b>Results:</b> PICTURE
 
<b>Notes:</b>
 
<b>Stop Time:</b> 12:33 PM
 
<b>Next:</b> TOPO cloning of 8/6/13 PCR products
 
== <b>Date: 08/07/13</b> <b>People in lab: Blythe Ferriere</b>  ==
 
<b><span style="font-size:16px;">Title:</span></b> TOPO cloning of PCR product and chemical transformation
 
<b>Start Time:</b> 2:45 PM
 
<b>Purpose:</b> To TOPO clone 8/6/13 A1 norV PCR product and chemically transform to amplify gene product
 
<b>Protocol:</b> TOPO cloning: Fresh PCR product 4uL, PCR TOPO vector 1uL. 1. Mix gently and incubate for 5 minutes at room temperature.
Transformation: LTM ed. 2 pg. 42 <b>Exceptions:</b> 1. 2uL of TOPO cloning reaction product 2. 1uL pUC19 control standard 3. Plate on amp+Xgal
 
 
<b>Products:</b>
{| border="1" cellpadding="5"
{| border="1" cellpadding="5"
|-
|-
! Sample Label !! Description !! Source Label !! Quantity
! Sample Label !! Description !! Source Label !! Quantity
|-
|-
| Entry || Entry || Entry || #
| || || ||  
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|-
| Entry || Entry || Entry || #
| || || ||
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<b>Results:</b>
<b>Notes:</b> PCR product was a day old. 1 hour 35 min recovery time.
 
<b>Notes:</b>  
 
<b>Stop Time:</b>


<b>Next:</b>
<b>Stop Time:</b> 3:40 PM


<b>Next:</b> TOPO cloning of 8/6/13 PCR products




Latest revision as of 23:43, 26 September 2017

Cyanobacteria Mediated Filtration of Coal Flue Gas Main project page
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Date: 08/07/13 People in lab: Blythe Ferriere

Title: Gel Electrophoresis of 8/6/13 A1-A6 PCR reactions

Start Time: 8:45 AM

Purpose: To check for norV gene product with prefix&suffix from PCR amplification.

Protocol: LTM ed. 2 pg 45-47

Well 1 2 3 4 5 6 7 8
Sample Ladder 8/6/13 A1 8/6/13 A2 8/6/13 A3 8/6/13 A4 8/6/13 A5 8/6/13 A6 Ladder


Products: N/A

Results: PICTURE

Notes:

Stop Time: 12:33 PM

Next: TOPO cloning of 8/6/13 PCR products

Date: 08/07/13 People in lab: Blythe Ferriere

Title: TOPO cloning of PCR product and chemical transformation

Start Time: 2:45 PM

Purpose: To TOPO clone 8/6/13 A1 norV PCR product and chemically transform to amplify gene product

Protocol: TOPO cloning: Fresh PCR product 4uL, PCR TOPO vector 1uL. 1. Mix gently and incubate for 5 minutes at room temperature. Transformation: LTM ed. 2 pg. 42 Exceptions: 1. 2uL of TOPO cloning reaction product 2. 1uL pUC19 control standard 3. Plate on amp+Xgal


Products:

Sample Label Description Source Label Quantity

Notes: PCR product was a day old. 1 hour 35 min recovery time.

Stop Time: 3:40 PM

Next: TOPO cloning of 8/6/13 PCR products



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