IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2013/08/07

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(Autocreate 2013/08/07 Entry for IGEM:Missouri_University_of_Science_and_Technology/2009/Notebook/Cyanobacteria_Mediated_Filtration_of_Coal_Flue)
(Date: MM/DD/YY People in lab: Yourname)
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== <b>Date: MM/DD/YY</b> <b>People in lab: Yourname</b>  ==
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== <b>Date: 08/07/13</b> <b>People in lab: Blythe Ferriere</b>  ==
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<b><span style="font-size:16px;">Title:</span></b> Fill in the fields on this page to create a new notebook entry.
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<b><span style="font-size:16px;">Title:</span></b> Gel Electrophoresis of 8/6/13 A1-A6 PCR reactions
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<b>Start Time:</b>
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<b>Start Time:</b> 8:45 AM
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<b>Purpose:</b>  
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<b>Purpose:</b> To check for norV gene product with prefix&suffix from PCR amplification.
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<b>Protocol:</b> <b>Exceptions:</b>
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<b>Protocol:</b> LTM ed. 2 pg 45-47
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<b>Products:</b>
 
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! Sample Label !! Description !! Source Label !! Quantity
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! Well !! 1 !! 2 !! 3 !! 4 !! 5 !! 6 !! 7 !! 8
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| Entry || Entry || Entry || #
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| Sample || Ladder || 8/6/13 A1 || 8/6/13 A2 || 8/6/13 A3 || 8/6/13 A4 || 8/6/13 A5 || 8/6/13 A6 || Ladder ||
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| Entry || Entry || Entry || #
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<b>Results:</b>  
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<b>Products:</b> N/A
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<b>Results:</b> PICTURE
<b>Notes:</b>  
<b>Notes:</b>  
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<b>Stop Time:</b>  
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<b>Stop Time:</b> 12:33 PM
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<b>Next:</b> TOPO cloning of 8/6/13 PCR products
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== <b>Date: 08/07/13</b> <b>People in lab: Blythe Ferriere</b>  ==
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<b><span style="font-size:16px;">Title:</span></b> TOPO cloning of PCR product and chemical transformation
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<b>Start Time:</b> 2:45 PM
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<b>Purpose:</b> To TOPO clone 8/6/13 A1 norV PCR product and chemically transform to amplify gene product
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<b>Protocol:</b> TOPO cloning: Fresh PCR product 4uL, PCR TOPO vector 1uL. 1. Mix gently and incubate for 5 minutes at room temperature.
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Transformation: LTM ed. 2 pg. 42 <b>Exceptions:</b> 1. 2uL of TOPO cloning reaction product 2. 1uL pUC19 control standard 3. Plate on amp+Xgal
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<b>Products:</b> 
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<b>Notes:</b> PCR product was a day old. 1 hour 35 min recovery time.
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<b>Next:</b>
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<b>Stop Time:</b> 3:40 PM
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<b>Next:</b> TOPO cloning of 8/6/13 PCR products

Revision as of 12:53, 11 February 2014

Cyanobacteria Mediated Filtration of Coal Flue Gas Main project page
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Date: 08/07/13 People in lab: Blythe Ferriere

Title: Gel Electrophoresis of 8/6/13 A1-A6 PCR reactions

Start Time: 8:45 AM

Purpose: To check for norV gene product with prefix&suffix from PCR amplification.

Protocol: LTM ed. 2 pg 45-47

Well 1 2 3 4 5 6 7 8
Sample Ladder 8/6/13 A1 8/6/13 A2 8/6/13 A3 8/6/13 A4 8/6/13 A5 8/6/13 A6 Ladder


Products: N/A

Results: PICTURE

Notes:

Stop Time: 12:33 PM

Next: TOPO cloning of 8/6/13 PCR products


Date: 08/07/13 People in lab: Blythe Ferriere

Title: TOPO cloning of PCR product and chemical transformation

Start Time: 2:45 PM

Purpose: To TOPO clone 8/6/13 A1 norV PCR product and chemically transform to amplify gene product

Protocol: TOPO cloning: Fresh PCR product 4uL, PCR TOPO vector 1uL. 1. Mix gently and incubate for 5 minutes at room temperature. Transformation: LTM ed. 2 pg. 42 Exceptions: 1. 2uL of TOPO cloning reaction product 2. 1uL pUC19 control standard 3. Plate on amp+Xgal


Products:

Notes: PCR product was a day old. 1 hour 35 min recovery time.

Stop Time: 3:40 PM

Next: TOPO cloning of 8/6/13 PCR products



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