IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2013/08/07: Difference between revisions
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== <b>Date: | == <b>Date: 08/07/13</b> <b>People in lab: Blythe Ferriere</b> == | ||
<b><span style="font-size:16px;">Title:</span></b> | <b><span style="font-size:16px;">Title:</span></b> Gel Electrophoresis of 8/6/13 A1-A6 PCR reactions | ||
<b>Start Time:</b> | <b>Start Time:</b> 8:45 AM | ||
<b>Purpose:</b> | <b>Purpose:</b> To check for norV gene product with prefix&suffix from PCR amplification. | ||
<b>Protocol:</b> | <b>Protocol:</b> LTM ed. 2 pg 45-47 | ||
{| border="1" cellpadding="5" | {| border="1" cellpadding="5" | ||
|- | |- | ||
! | ! Well !! 1 !! 2 !! 3 !! 4 !! 5 !! 6 !! 7 !! 8 | ||
|- | |- | ||
| | | Sample || Ladder || 8/6/13 A1 || 8/6/13 A2 || 8/6/13 A3 || 8/6/13 A4 || 8/6/13 A5 || 8/6/13 A6 || Ladder || | ||
| | |} | ||
| | |||
|} | |||
<b>Products:</b> N/A | |||
<b>Results:</b> | <b>Results:</b> PICTURE | ||
<b>Notes:</b> | <b>Notes:</b> | ||
<b>Stop Time:</b> | <b>Stop Time:</b> 12:33 PM | ||
<b>Next:</b> TOPO cloning of 8/6/13 PCR products | |||
== <b>Date: 08/07/13</b> <b>People in lab: Blythe Ferriere</b> == | |||
<b><span style="font-size:16px;">Title:</span></b> TOPO cloning of PCR product and chemical transformation | |||
<b>Start Time:</b> 2:45 PM | |||
<b>Purpose:</b> To TOPO clone 8/6/13 A1 norV PCR product and chemically transform to amplify gene product | |||
<b>Protocol:</b> TOPO cloning: Fresh PCR product 4uL, PCR TOPO vector 1uL. 1. Mix gently and incubate for 5 minutes at room temperature. | |||
Transformation: LTM ed. 2 pg. 42 <b>Exceptions:</b> 1. 2uL of TOPO cloning reaction product 2. 1uL pUC19 control standard 3. Plate on amp+Xgal | |||
<b>Products:</b> | |||
<b>Notes:</b> PCR product was a day old. 1 hour 35 min recovery time. | |||
<b> | <b>Stop Time:</b> 3:40 PM | ||
<b>Next:</b> TOPO cloning of 8/6/13 PCR products | |||
Revision as of 09:53, 11 February 2014
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Date: 08/07/13 People in lab: Blythe FerriereTitle: Gel Electrophoresis of 8/6/13 A1-A6 PCR reactions Start Time: 8:45 AM Purpose: To check for norV gene product with prefix&suffix from PCR amplification. Protocol: LTM ed. 2 pg 45-47
Results: PICTURE Notes: Stop Time: 12:33 PM Next: TOPO cloning of 8/6/13 PCR products
Date: 08/07/13 People in lab: Blythe FerriereTitle: TOPO cloning of PCR product and chemical transformation Start Time: 2:45 PM Purpose: To TOPO clone 8/6/13 A1 norV PCR product and chemically transform to amplify gene product Protocol: TOPO cloning: Fresh PCR product 4uL, PCR TOPO vector 1uL. 1. Mix gently and incubate for 5 minutes at room temperature. Transformation: LTM ed. 2 pg. 42 Exceptions: 1. 2uL of TOPO cloning reaction product 2. 1uL pUC19 control standard 3. Plate on amp+Xgal
Notes: PCR product was a day old. 1 hour 35 min recovery time. Stop Time: 3:40 PM Next: TOPO cloning of 8/6/13 PCR products
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