IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2013/08/08: Difference between revisions

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|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:20px;"> Cyanobacteria Mediated Filtration of Coal Flue Gas</span>
|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:20px;"> Cyanobacteria Mediated Filtration of Coal Flue Gas</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
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== <b>Date: MM/DD/YY</b> <b>People in lab: Yourname</b>  ==
== <b>Date: 08/08/13</b> <b>People in lab: Blythe Ferriere</b>  ==


<b><span style="font-size:16px;">Title:</span></b> Fill in the fields on this page to create a new notebook entry.
<b><span style="font-size:16px;">Title:</span></b> Blue/white screening for norV TOPO-TA cloned colonies


<b>Start Time:</b>
<b>Start Time:</b> 4:10PM


<b>Purpose:</b>  
<b>Purpose:</b> Plate screened for blue white colonies white colonies should have plasmid.


<b>Protocol:</b> <b>Exceptions:</b>
<b>Protocol:</b> Colonies from plate CT1 8/7/13 were inoculated into LB broth + ampicillin tubes


<b>Products:</b>  
<b>Products:</b>  
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! Sample Label !! Description !! Source Label !! Quantity
! Sample Label !! Description !! Source Label !! Quantity
|-
|-
| Entry || Entry || Entry || #
| 8/8/13 I 1 || Isolation and inoculation of white colony || 8/7/13 CT1 || 8
|-
|-
| Entry || Entry || Entry || #
| 8/8/13 I 9 || Isolation and inoculation of light blue colony || 8/7/13 CT1 || 1
|-
| 8/8/13 I 10 || Isolation and inoculation of blue colony || 8/7/13 CT1 || 3
|}
|}


<b>Results:</b>  
<b>Notes:</b> Ampicillin re-done in I1-I8
 
<b>Stop Time:</b> 5:41 PM
 
<b>Next:</b> Pick tubes with growth from white colonies for further analysis for check for norV gene
 
 
== <b>Date: 08/08/13</b> <b>People in lab: Emily Puleo</b>  ==
 
<b><span style="font-size:16px;">Title:</span></b> Gel electrophoresis of 8/7/13 PCR samples
 
<b>Start Time:</b> 4:37 PM
 
<b>Purpose:</b> To check for successful PCR reaction.
 
<b>Protocol:</b> LTM ed. 2 pg. 44
 
{| border="1" cellpadding="5"
|-
! Well !! 1 !! 2 !! 3 !! 4 !! 5 !! 6 !! 7 !! 8
|-
| Sample || Ladder || 8/7/13 A norCB || 8/7/13 A nosZ 1 || 8/7/13 A nosZ 2 || 8/7/13 A hmp 1 || 8/7/13 A hmp 2 ||  ||
|}
 
<b>Results:</b> Product from hmp only. PICTURE
 
<b>Notes:</b> Used 70 uL sample+LoadingDye per well


<b>Notes:</b>  
<b>Stop Time:</b> 5:20PM
 
== <b>Date: 08/08/13</b> <b>People in lab: Emily Puleo</b>  ==
 
<b><span style="font-size:16px;">Title:</span></b> TOPO-TA cloning of 8/7/13 PCR samples
 
<b>Start Time:</b> 5:05 PM
 
<b>Purpose:</b> To put genes into vectors and transform into bacteria to amplify.
 
<b>Protocol:</b> Invitrogen TOPO-TA cloning manual
 
<b>Products:</b>
{| border="1" cellpadding="5"
|-
! Sample Label !! Description !! Source Label !! Quantity
|-
| 8/8/13 hmp L1 || hmp in TOPO-TA cloning vector || 8/7/13 hmp A1 || 1
|-
|}
 
<b>Notes:</b> Samples were 1 day old only one was completed because only one ampicillin plate was ready
 
<b>Stop Time:</b> 6:15 PM
 
== <b>Date: 08/08/13</b> <b>People in lab: Emily Puleo</b>  ==
 
<b><span style="font-size:16px;">Title:</span></b> Transformation of 8/8/13 hmp L1
 
<b>Start Time:</b> 6:10 PM
 
<b>Purpose:</b> To put the hmp-TOPO vector into DH5alpha cells for growth and selection.


<b>Stop Time:</b>  
<b>Protocol:</b> LTM ed. 2 pg 41


<b>Next:</b>
<b>Products:</b>  
{| border="1" cellpadding="5"
|-
! Sample Label !! Description !! Source Label !! Quantity
|-
| 8/8/13 hmp T || hmp in TOPO-TA cloning vector in DH5alpha plated on LB+amp || 8/8/13 hmp L1 || 1
|-
|}


<b>Notes:</b>


<b>Stop Time:</b> 9:30 PM


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Latest revision as of 23:50, 26 September 2017

Cyanobacteria Mediated Filtration of Coal Flue Gas Main project page
Previous entry      Next entry

Date: 08/08/13 People in lab: Blythe Ferriere

Title: Blue/white screening for norV TOPO-TA cloned colonies

Start Time: 4:10PM

Purpose: Plate screened for blue white colonies white colonies should have plasmid.

Protocol: Colonies from plate CT1 8/7/13 were inoculated into LB broth + ampicillin tubes

Products:

Sample Label Description Source Label Quantity
8/8/13 I 1 Isolation and inoculation of white colony 8/7/13 CT1 8
8/8/13 I 9 Isolation and inoculation of light blue colony 8/7/13 CT1 1
8/8/13 I 10 Isolation and inoculation of blue colony 8/7/13 CT1 3

Notes: Ampicillin re-done in I1-I8

Stop Time: 5:41 PM

Next: Pick tubes with growth from white colonies for further analysis for check for norV gene


Date: 08/08/13 People in lab: Emily Puleo

Title: Gel electrophoresis of 8/7/13 PCR samples

Start Time: 4:37 PM

Purpose: To check for successful PCR reaction.

Protocol: LTM ed. 2 pg. 44

Well 1 2 3 4 5 6 7 8
Sample Ladder 8/7/13 A norCB 8/7/13 A nosZ 1 8/7/13 A nosZ 2 8/7/13 A hmp 1 8/7/13 A hmp 2

Results: Product from hmp only. PICTURE

Notes: Used 70 uL sample+LoadingDye per well

Stop Time: 5:20PM

Date: 08/08/13 People in lab: Emily Puleo

Title: TOPO-TA cloning of 8/7/13 PCR samples

Start Time: 5:05 PM

Purpose: To put genes into vectors and transform into bacteria to amplify.

Protocol: Invitrogen TOPO-TA cloning manual

Products:

Sample Label Description Source Label Quantity
8/8/13 hmp L1 hmp in TOPO-TA cloning vector 8/7/13 hmp A1 1

Notes: Samples were 1 day old only one was completed because only one ampicillin plate was ready

Stop Time: 6:15 PM

Date: 08/08/13 People in lab: Emily Puleo

Title: Transformation of 8/8/13 hmp L1

Start Time: 6:10 PM

Purpose: To put the hmp-TOPO vector into DH5alpha cells for growth and selection.

Protocol: LTM ed. 2 pg 41

Products:

Sample Label Description Source Label Quantity
8/8/13 hmp T hmp in TOPO-TA cloning vector in DH5alpha plated on LB+amp 8/8/13 hmp L1 1

Notes:

Stop Time: 9:30 PM


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