IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2013/08/08: Difference between revisions
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|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:20px;"> Cyanobacteria Mediated Filtration of Coal Flue Gas</span> | |style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:20px;"> Cyanobacteria Mediated Filtration of Coal Flue Gas</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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Results: Product from hmp only. PICTURE | <b>Results:</b> Product from hmp only. PICTURE | ||
Notes: Used 70 uL sample+LoadingDye per well | <b>Notes:</b> Used 70 uL sample+LoadingDye per well | ||
<b>Stop Time:</b> 5:20PM | |||
== <b>Date: 08/08/13</b> <b>People in lab: Emily Puleo</b> == | == <b>Date: 08/08/13</b> <b>People in lab: Emily Puleo</b> == | ||
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! Sample Label !! Description !! Source Label !! Quantity | ! Sample Label !! Description !! Source Label !! Quantity | ||
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| 8/8/13 hmp L1 || hmp in TOPO-TA cloning vector | | 8/8/13 hmp L1 || hmp in TOPO-TA cloning vector || 8/7/13 hmp A1 || 1 | ||
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<b>Stop Time:</b> 6:15 PM | <b>Stop Time:</b> 6:15 PM | ||
== <b>Date: 08/08/13</b> <b>People in lab: Emily Puleo</b> == | |||
<b><span style="font-size:16px;">Title:</span></b> Transformation of 8/8/13 hmp L1 | |||
<b>Start Time:</b> 6:10 PM | |||
<b>Purpose:</b> To put the hmp-TOPO vector into DH5alpha cells for growth and selection. | |||
<b>Protocol:</b> LTM ed. 2 pg 41 | |||
<b>Products:</b> | |||
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! Sample Label !! Description !! Source Label !! Quantity | |||
|- | |||
| 8/8/13 hmp T || hmp in TOPO-TA cloning vector in DH5alpha plated on LB+amp || 8/8/13 hmp L1 || 1 | |||
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<b>Notes:</b> | |||
<b>Stop Time:</b> 9:30 PM | |||
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Latest revision as of 23:50, 26 September 2017
Cyanobacteria Mediated Filtration of Coal Flue Gas | Main project page Previous entry Next entry | ||||||||||||||||||||||||||||||||||||||||||||||||||
Date: 08/08/13 People in lab: Blythe FerriereTitle: Blue/white screening for norV TOPO-TA cloned colonies Start Time: 4:10PM Purpose: Plate screened for blue white colonies white colonies should have plasmid. Protocol: Colonies from plate CT1 8/7/13 were inoculated into LB broth + ampicillin tubes Products:
Notes: Ampicillin re-done in I1-I8 Stop Time: 5:41 PM Next: Pick tubes with growth from white colonies for further analysis for check for norV gene
Date: 08/08/13 People in lab: Emily PuleoTitle: Gel electrophoresis of 8/7/13 PCR samples Start Time: 4:37 PM Purpose: To check for successful PCR reaction. Protocol: LTM ed. 2 pg. 44
Results: Product from hmp only. PICTURE Notes: Used 70 uL sample+LoadingDye per well Stop Time: 5:20PM Date: 08/08/13 People in lab: Emily PuleoTitle: TOPO-TA cloning of 8/7/13 PCR samples Start Time: 5:05 PM Purpose: To put genes into vectors and transform into bacteria to amplify. Protocol: Invitrogen TOPO-TA cloning manual Products:
Notes: Samples were 1 day old only one was completed because only one ampicillin plate was ready Stop Time: 6:15 PM Date: 08/08/13 People in lab: Emily PuleoTitle: Transformation of 8/8/13 hmp L1 Start Time: 6:10 PM Purpose: To put the hmp-TOPO vector into DH5alpha cells for growth and selection. Protocol: LTM ed. 2 pg 41 Products:
Notes: Stop Time: 9:30 PM |