IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2013/08/08: Difference between revisions
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Levi Palmer (talk | contribs) (Autocreate 2013/08/08 Entry for IGEM:Missouri_University_of_Science_and_Technology/2009/Notebook/Cyanobacteria_Mediated_Filtration_of_Coal_Flue) |
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== <b>Date: | == <b>Date: 08/08/13</b> <b>People in lab: Blythe Ferriere</b> == | ||
<b><span style="font-size:16px;">Title:</span></b> | <b><span style="font-size:16px;">Title:</span></b> Blue/white screening for norV TOPO-TA cloned colonies | ||
<b>Start Time:</b> | <b>Start Time:</b> 4:10PM | ||
<b>Purpose:</b> | <b>Purpose:</b> Plate screened for blue white colonies white colonies should have plasmid. | ||
<b>Protocol:</b> | <b>Protocol:</b> Colonies from plate CT1 8/7/13 were inoculated into LB broth + ampicillin tubes | ||
<b>Products:</b> | <b>Products:</b> | ||
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! Sample Label !! Description !! Source Label !! Quantity | ! Sample Label !! Description !! Source Label !! Quantity | ||
|- | |- | ||
| | | 8/8/13 I 1 || Isolation and inoculation of white colony || 8/7/13 CT1 || 8 | ||
|- | |- | ||
| | | 8/8/13 I 9 || Isolation and inoculation of light blue colony || 8/7/13 CT1 || 1 | ||
|- | |||
| 8/8/13 I 10 || Isolation and inoculation of blue colony || 8/7/13 CT1 || 3 | |||
|} | |} | ||
<b>Notes:</b> Ampicillin re-done in I1-I8 | |||
<b>Notes:</b> | |||
<b>Stop Time:</b> | <b>Stop Time:</b> 5:41 PM | ||
<b>Next:</b> | <b>Next:</b> Pick tubes with growth from white colonies for further analysis for check for norV gene | ||
Revision as of 13:20, 20 March 2014
 Cyanobacteria Mediated Filtration of Coal Flue Gas
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Date: 08/08/13 People in lab: Blythe FerriereTitle: Blue/white screening for norV TOPO-TA cloned colonies Start Time: 4:10PM Purpose: Plate screened for blue white colonies white colonies should have plasmid. Protocol: Colonies from plate CT1 8/7/13 were inoculated into LB broth + ampicillin tubes Products:
Notes: Ampicillin re-done in I1-I8 Stop Time: 5:41 PM Next: Pick tubes with growth from white colonies for further analysis for check for norV gene
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