IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2013/08/08: Difference between revisions
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<b>Protocol:</b> Invitrogen TOPO-TA cloning manual | <b>Protocol:</b> Invitrogen TOPO-TA cloning manual | ||
<b>Products:</b> {| border="1" cellpadding="5" | <b>Products:</b> | ||
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! Sample Label !! Description !! Source Label !! Quantity | ! Sample Label !! Description !! Source Label !! Quantity | ||
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| 8/8/13 hmp L1 || hmp in TOPO-TA cloning vector on ampicillin || 8/7/13 hmp A1 || 1 | | 8/8/13 hmp L1 || hmp in TOPO-TA cloning vector on ampicillin || 8/7/13 hmp A1 || 1 | ||
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Revision as of 14:07, 20 March 2014
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Date: 08/08/13 People in lab: Blythe FerriereTitle: Blue/white screening for norV TOPO-TA cloned colonies Start Time: 4:10PM Purpose: Plate screened for blue white colonies white colonies should have plasmid. Protocol: Colonies from plate CT1 8/7/13 were inoculated into LB broth + ampicillin tubes Products:
Notes: Ampicillin re-done in I1-I8 Stop Time: 5:41 PM Next: Pick tubes with growth from white colonies for further analysis for check for norV gene
Date: 08/08/13 People in lab: Emily PuleoTitle: Gel electrophoresis of 8/7/13 PCR samples Start Time: 4:37 PM Purpose: To check for successful PCR reaction. Protocol: LTM ed. 2 pg. 44
Results: Product from hmp only. PICTURE Notes: Used 70 uL sample+LoadingDye per well Stop: 5:20PM Date: 08/08/13 People in lab: Emily PuleoTitle: TOPO-TA cloning of 8/7/13 PCR samples Start Time: 5:05 PM Purpose: To put genes into vectors and transform into bacteria to amplify. Protocol: Invitrogen TOPO-TA cloning manual Products:
Notes: Samples were 1 day old only one was completed because only one ampicillin plate was ready Stop Time: 6:15 PM |